Data/hora: 23/06/2024 18:13:47

Biblioteca(s): | Embrapa Uva e Vinho.
Data corrente: | 11/03/2005
Data da última atualização: | 13/11/2019
Autoria: | BRONOMELLI, A.; MERCIER, L.; FRANCHEL, J.; BAILLIEUL, F.; BENIZRI, E.; MAURO, M.-C.
Título: | Response of grapevine defenses to UV-C exposure.
Ano de publicação: | 2004
Fonte/Imprenta: | American Journal of Enology and Viticulture, Davis, v. 55, n. 1, p. 51-59, 2004.
Páginas: | p. 51-59
Idioma: | Inglês
Conteúdo: | Abstract: The defense potential of a tolerant American Yitis rupestris cultivar (Rupestris du Lot) and a susceptible European Vitis vinifera cultivar (Chardonnay) in response to UV-C irradiation was investigated, The expression of eight defense-related genes coding for enzymes of the phenylpropanoid pathway (phenylalanine ammonia Jyase and stilbene synthase), the octadecanoid pathway (lipoxygenase), and pathogenesis-related proteins (class I and Ill chitinases, B-I ,3-glllcanase, cIass 6 pathogenesis-related protein, and class 10 pathogenesis-related protein) was followed by real-time reverse transcription polymerase chain reaction (RT-PCR). Phenolic compound accumulation was monitored by microscopic observation. Accumulation of resveratrol, a major grapevine phytoalexin, was evaluated by HPLC, and chitinase and fi-I ,3- glucanase enzyme activities were measured. Both grapevine species responded to UV-C treatment by enhancement of defense mechanisms. Intensity of some defense responses was correlated with tolerance to diseases, as previously described for stilbene accumulation: the tolerant species responded more intensely to UV-C exposure than the susceptibJe one. UV-C irradiation is a practical and reproducible method for inducing grapevine defense responses and can be useful in determining the defense potentiaJ of grapevine cultivars. Keys words: grapevine, UV-C (200 to 280 nm), defense mechanisms, real-time RT-PCR Abbreviations: class I chitinase (Chitlb); class III chitinase (CH3); Iipoxygenase (LOX); phenylalanine ammonia lyase (PAL); class 6 pathogenesis-related protein (PR-6); class 10 pathogenesis-reJated protein (PR-IO); stilbene synthase (StSy)
Palavras-Chave: | Mecanismo de defesa; Radiação UV-C.
Thesagro: | Uva; Variedade; Viticultura.
Categoria do assunto: | --
Registro original: | Embrapa Uva e Vinho (CNPUV)
Exemplares: | CNPUV - (6636-1) - ADC - AP - DD -  -  - 05.00011 - DIS