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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
01/04/1998 |
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Data da última atualização: |
11/08/2014 |
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Autoria: |
BAHARUDDIN, B.; RUDOLPH, K.; NIEPOLD, F. |
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Título: |
Production of monospecific antiserum against the blood disease bacterium affecting banana and plantain. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Phytopathology, v. 84, n. 6, p. 570-575, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
Proteins from the blood disease bacterium (BDB) affecting banana and plantain and proteins from other bacterial species were separated by sodium dodeecyl sulfate-polyacrylamide gel electrophoresis, blotted onto nitrocellulose membrane, and probed with polycional antibodies directed against BDB A 33.5-kDa protein band was characteristic for all virulent strains of BDB examined. Monospecific antibodies obtained against this protein by affinity purification were specific for BDB. A second polyclonal antibody was raised against the gel-purified 33-kDa protein in a rabbit. The serum was termed monospecific antiserum (MSA) and was used in subsequent experiments. The MSA reacted specifically with all virulent strains of BDB in agglutination tests and colony blot tests but not or only weakly with Pseudomonas solanacearum, P. syzygii, and other bacterial species. The specificity of the MSA was confirmed in studies with banana plant extracts. BDB could be detected in all infected plants 3-33 days after inoculation, whereas plants inoculated with P. solanacearum race 2 did not react with the MSA on immunocolony blots. |
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Palavras-Chave: |
Diagnostico diferencial; Doenca bacteriana; Pseudomonas solanacearum; Wilt disease. |
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Thesagro: |
Banana; Zoologia. |
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Thesaurus Nal: |
serology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01771naa a2200229 a 4500
001 1049757
005 2014-08-11
008 1994 bl --- 0-- u #d
100 1 $aBAHARUDDIN, B.
245 $aProduction of monospecific antiserum against the blood disease bacterium affecting banana and plantain.
260 $c1994
520 $aProteins from the blood disease bacterium (BDB) affecting banana and plantain and proteins from other bacterial species were separated by sodium dodeecyl sulfate-polyacrylamide gel electrophoresis, blotted onto nitrocellulose membrane, and probed with polycional antibodies directed against BDB A 33.5-kDa protein band was characteristic for all virulent strains of BDB examined. Monospecific antibodies obtained against this protein by affinity purification were specific for BDB. A second polyclonal antibody was raised against the gel-purified 33-kDa protein in a rabbit. The serum was termed monospecific antiserum (MSA) and was used in subsequent experiments. The MSA reacted specifically with all virulent strains of BDB in agglutination tests and colony blot tests but not or only weakly with Pseudomonas solanacearum, P. syzygii, and other bacterial species. The specificity of the MSA was confirmed in studies with banana plant extracts. BDB could be detected in all infected plants 3-33 days after inoculation, whereas plants inoculated with P. solanacearum race 2 did not react with the MSA on immunocolony blots.
650 $aserology
650 $aBanana
650 $aZoologia
653 $aDiagnostico diferencial
653 $aDoenca bacteriana
653 $aPseudomonas solanacearum
653 $aWilt disease
700 1 $aRUDOLPH, K.
700 1 $aNIEPOLD, F.
773 $tPhytopathology$gv. 84, n. 6, p. 570-575, 1994.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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