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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
06/03/2015 |
Data da última atualização: |
06/03/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SICHERLE, C. C.; MAIA, M. da S.; BICUDO, S. D.; RODELLO, L.; AZEVEDO, H. C. |
Afiliação: |
C. C. SICHERLE, São Paulo State University, Faculty of Veterinary Medicine and Animal Science; MARCIANE DA SILVA MAIA, CPATSA; S. D. BICUDO, São Paulo State University, Faculty of Veterinary Medicine and Animal Science; L. RODELLO, São Paulo State University, Faculty of Veterinary Medicine and Animal Science; H. C. AZEVEDO, São Paulo State University, Faculty of Veterinary Medicine and Animal Science. |
Título: |
Lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen supplemented with catalase or Trolox. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Small Ruminant Research, v. 95, p. 144-149, 2011. |
Idioma: |
Inglês |
Conteúdo: |
Semen manipulation and cryopreservation-thaw proceduresmayaccelerate the generation of reactive oxygen species (ROS). Sperm exposure to large amounts of ROS has been shown to cause membrane lipid peroxidation and cellular injury to the sperm. The objective of this study was to overcome the ROS production in frozen-thawed ram semen by the addition of the antioxidants catalase or Trolox to semen following thawing. Frozen-thawed ram semen (100×106 sperm/straw) was supplemented with PBS (control group), 100g/ml catalase, or 100MTrolox/108 sperm (catalase and Trolox being dissolved in PBS) and incubated (37 ◦C) for 5 min. Under the experimental conditions used in this study, the catalase and Trolox antioxidants failed to protect the sperm from the spontaneous production of ROS. However, when lipid peroxidation was induced by iron (FeSO4), the addition of Trolox promoted a reduction (P < 0.05) in the formation of TBARS in the semen, compared to the control and catalase semen samples. The generation of TBARS and H2O2 occurred in the extender alone, without the presence of sperm cells. In conclusion, the addition of Trolox to frozen-thawed ram semen could be beneficial as it decreases the production of TBARS when oxidative stress is induced. It is possible that a longer incubation period could lead to different results. The concentration of catalase also needs to be further evaluated. The extender could contribute to the oxidative stress of sperm, as it is a source of ROS during the cryopreservation of semen. MenosSemen manipulation and cryopreservation-thaw proceduresmayaccelerate the generation of reactive oxygen species (ROS). Sperm exposure to large amounts of ROS has been shown to cause membrane lipid peroxidation and cellular injury to the sperm. The objective of this study was to overcome the ROS production in frozen-thawed ram semen by the addition of the antioxidants catalase or Trolox to semen following thawing. Frozen-thawed ram semen (100×106 sperm/straw) was supplemented with PBS (control group), 100g/ml catalase, or 100MTrolox/108 sperm (catalase and Trolox being dissolved in PBS) and incubated (37 ◦C) for 5 min. Under the experimental conditions used in this study, the catalase and Trolox antioxidants failed to protect the sperm from the spontaneous production of ROS. However, when lipid peroxidation was induced by iron (FeSO4), the addition of Trolox promoted a reduction (P < 0.05) in the formation of TBARS in the semen, compared to the control and catalase semen samples. The generation of TBARS and H2O2 occurred in the extender alone, without the presence of sperm cells. In conclusion, the addition of Trolox to frozen-thawed ram semen could be beneficial as it decreases the production of TBARS when oxidative stress is induced. It is possible that a longer incubation period could lead to different results. The concentration of catalase also needs to be further evaluated. The extender could contribute to the oxidative stress of sperm, as it is a source of ROS duri... Mostrar Tudo |
Palavras-Chave: |
Semen cryopreservation; Sêmen ovino congelado-descongelado. |
Thesagro: |
Catalase; Ovino; Produção animal. |
Thesaurus Nal: |
ANimal production; Lipid peroxidation. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02313naa a2200253 a 4500 001 2010784 005 2015-03-06 008 2011 bl uuuu u00u1 u #d 100 1 $aSICHERLE, C. C. 245 $aLipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen supplemented with catalase or Trolox.$h[electronic resource] 260 $c2011 520 $aSemen manipulation and cryopreservation-thaw proceduresmayaccelerate the generation of reactive oxygen species (ROS). Sperm exposure to large amounts of ROS has been shown to cause membrane lipid peroxidation and cellular injury to the sperm. The objective of this study was to overcome the ROS production in frozen-thawed ram semen by the addition of the antioxidants catalase or Trolox to semen following thawing. Frozen-thawed ram semen (100×106 sperm/straw) was supplemented with PBS (control group), 100g/ml catalase, or 100MTrolox/108 sperm (catalase and Trolox being dissolved in PBS) and incubated (37 ◦C) for 5 min. Under the experimental conditions used in this study, the catalase and Trolox antioxidants failed to protect the sperm from the spontaneous production of ROS. However, when lipid peroxidation was induced by iron (FeSO4), the addition of Trolox promoted a reduction (P < 0.05) in the formation of TBARS in the semen, compared to the control and catalase semen samples. The generation of TBARS and H2O2 occurred in the extender alone, without the presence of sperm cells. In conclusion, the addition of Trolox to frozen-thawed ram semen could be beneficial as it decreases the production of TBARS when oxidative stress is induced. It is possible that a longer incubation period could lead to different results. The concentration of catalase also needs to be further evaluated. The extender could contribute to the oxidative stress of sperm, as it is a source of ROS during the cryopreservation of semen. 650 $aANimal production 650 $aLipid peroxidation 650 $aCatalase 650 $aOvino 650 $aProdução animal 653 $aSemen cryopreservation 653 $aSêmen ovino congelado-descongelado 700 1 $aMAIA, M. da S. 700 1 $aBICUDO, S. D. 700 1 $aRODELLO, L. 700 1 $aAZEVEDO, H. C. 773 $tSmall Ruminant Research$gv. 95, p. 144-149, 2011.
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Embrapa Semiárido (CPATSA) |
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Registros recuperados : 320 | |
47. | | MARIA, A. N.; AZEVEDO, H. C.; SANTOS, J. P.; CARNEIRO, P. C. F. Avaliação da viabilidade espermática em tambaqui: determinação do tempo de incubação do sêmem em sondas fluorescentes. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304). Editora técnica Clara Oliveira Goedert. Resumo simles.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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48. | | NOZAKI, C.; AZEVEDO, H. C.; LIRA, N.; RODELLO, L.; AUGUSTO FILHO, O.; BICUDO, S.; MEGID, J. Brucella ovis in semn and urine of experimentally infected rams: correlation with the serological test and potential as a sourse of infection. In: JUBILEE WORLD BUIATRICS CONGRESS, 25., 2008, Budapest. Abstracts... Budapest: LAPJA, 2008.Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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49. | | BCHARA, M.; MCMANUS, C.; BLACKBURN, H.; AZEVEDO, H. C.; CAETANO, A. R.; PAIVA, S. R. Validação de um painel reduzido de marcadores moleculares associados com susceptibilidade ao vírus da pneumonia ovina. Revista RG News, v. 4, n. 3, p. 83, 2018. Edição especial dos Anais do 5 Congresso Brasileiro de Recursos Genéticos, Fortaleza, nov. 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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50. | | BCHARA, M.; MCMANUS, C.; BLACKBURN, H.; AZEVEDO, H. C.; CAETANO, A. R.; PAIVA, S. R. Validação de um painel reduzido de marcadores moleculares associados com susceptibilidade ao vírus da pneumonia ovina. Revista RG News, v. 4, n. 3, p. 83, 2018. Edição especial dos Anais do 5 Congresso Brasileiro de Recursos Genéticos, Fortaleza, nov. 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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51. | | FARIA, D. A.; BCHARA, M.; MCMANUS, C.; BLACKBURN, H.; AZEVEDO, H. C.; PAIVA, S. R. Validação em um painel reduzido de marcadores moleculares relacionados à susceptibilidade ao scrapie em ovinos. Revista RG News, v. 4, n. 3, p. 84, 2018. Edição especial com os Anais do V Congresso Brasileiro de Recursos Genéticos.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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52. | | FARIA, D. A.; BCHARA, M.; MCMANUS, C.; BLACKBURN, H.; AZEVEDO, H. C.; PAIVA, S. R. Validação de um painel reduzido de marcadores moleculares relacionados à susceptibilidade ao scrapie em ovinos. Revista RG News, v. 4, n. 3, p. 83, 2018. Edição especial dos Anais do 5 Congresso Brasileiro de Recursos Genéticos, Fortaleza, nov. 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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56. | | AZEVEDO, H. C.; SALLES, H. O.; MACHADO, R.; SOARES, A. T.; SANTOS, D. O. Efeito da associação norgestomet-cloprostenol na indução do parto em cabras leiteiras. ARS Veterinária, Jaboticabal, v. 14, n. 2, p. 134-139, 1998.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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58. | | CARNEIRO, P. C. F.; AZEVEDO, H. C.; SANTOS, J. P.; MARIA, A. N. Cryopreservation of tambaqui (Colossoma macropomum) semen: extenders, cryoprotectants, dilution ratios and freezing methods. Cryo-Letters, v. 33, n. 5, p. 385-393, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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