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| 41. |  | COLONNEELLI, B. L.; CAMARAO, A. P.; AZEVEDO, G. P. C. de; RODRIGUES FILHO, J. A. Avaliação de pastagens de capim braquiarão (Brachiaria brizantha, cv. Marandu) sob sistema de manejo rotativo para produção de leite em área de pequeno produtor. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA FCAP, 12.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 6., 2002, Belém, PA. A contribuição do profissional de Ciências Agrárias no uso e conservação da biodiversidade: anais. Belém, PA: FCAP: Embrapa Amazônia Oriental, 2002. Cópia de trabalho editado em CD-ROM| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 47. | | CAMARÃO, A. P.; AZEVEDO, G. P. C. de; SERRÃO, E. A. S. Fosforo, leguminosas, quicuio da Amazônia e manejo no melhoramento de pastagem de capim colonião em São João do Araguaia, Pará. In: REUNIÃO DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 18., 1981, Goiânia. Anais. Goiânia: SBZ, 1981. p. 31.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 51. | | RODRIGUES FILHO, J. A.; AZEVEDO, G. P. C. de; VEIGA, J. B. da; CAMARÃO, A. P. Recuperação de pastagens nos sistemas de produção leiteira no estado do Pará. Amazônia: ciência & desenvolvimento, Belém, PA, v. 4, n. 8, p. 229-234, jan./jun. 2009.| Biblioteca(s): Embrapa Amazônia Oriental. |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
06/05/2019 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
COELHO, S. V. B.; ROSA, S. D. V. F. da; FANTAZZINI, T. B.; BAUTE, J. L.; SILVA, L. C. |
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Afiliação: |
Stefânia Vilas Boas Coelho, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Tatiana Botelho Fantazzini, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Júlia Lima Baute, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Luciano Coutinho Silva, Departamento de Biologia Celular e Molecular/Universidade Federal da Paraíba - UFPB. |
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Título: |
Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v. 42, n. 6, p. 588-597, Nov/Dec. 2018 |
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Idioma: |
Português |
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Notas: |
Título em português Criopreservação em sementes de Coffea canephora Pierre: Resfriamento lento e rápido. |
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Conteúdo: |
Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality. MenosCoffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Cat... Mostrar Tudo |
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Palavras-Chave: |
Cristal de gelo; Drying in silica gel; Ice crystals; Isoenzymes; Physiological quality; Qualidade fisiológica; Secagem em sílica gel; Semente recalcitrante. |
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Thesagro: |
Isoenzima; Secagem. |
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Thesaurus NAL: |
Recalcitrant seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196979/1/Cryopreservation-in-Coffea-canephora-Pierre-seeds.pdf
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Marc: |
LEADER 02666naa a2200313 a 4500
001 2108759
005 2019-05-06
008 2018 bl uuuu u00u1 u #d
100 1 $aCOELHO, S. V. B.
245 $aCryopreservation in Coffea canephora Pierre seeds$bslow and fast cooling.$h[electronic resource]
260 $c2018
500 $aTítulo em português Criopreservação em sementes de Coffea canephora Pierre: Resfriamento lento e rápido.
520 $aCoffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality.
650 $aRecalcitrant seeds
650 $aIsoenzima
650 $aSecagem
653 $aCristal de gelo
653 $aDrying in silica gel
653 $aIce crystals
653 $aIsoenzymes
653 $aPhysiological quality
653 $aQualidade fisiológica
653 $aSecagem em sílica gel
653 $aSemente recalcitrante
700 1 $aROSA, S. D. V. F. da
700 1 $aFANTAZZINI, T. B.
700 1 $aBAUTE, J. L.
700 1 $aSILVA, L. C.
773 $tCiência e Agrotecnologia$gv. 42, n. 6, p. 588-597, Nov/Dec. 2018
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