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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/11/2020 |
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Data da última atualização: |
29/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, J. F.; ANDRIOLI, A.; PINHEIRO, R. R.; SIDER, L. H.; SOUSA, A. L. M. de; AZEVEDO, D. A. A. de; PEIXOTO, R. M.; LIMA, A. M. C.; DAMASCENO, E. M.; SOUZA, S. C. R.; TEIXEIRA, M. F. da S. |
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Afiliação: |
JUSCILÂNIA FURTADO ARAÚJO; ALICE ANDRIOLI, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; LUCIA HELENA SIDER, CNPC; ANA LÍDIA MADEIRA DE SOUSA; DALVA ALANA ARAGÃO DE AZEVEDO; RENATO MESQUITA PEIXOTO; ANA MILENA CESAR LIMA; EDGAR MARQUES DAMASCENO; SAMARA CRISTINA ROCHA SOUZA; MARIA FÁTIMA DA SILVA TEIXEIRA. |
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Título: |
Vertical transmissibility of small ruminant lentivirus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
PLoSONE, v. 15, n. 11, e0239916, Nov. 2020. |
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DOI: |
https://doi.org/10.1371/journal.pone.0239916 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats. MenosAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in t... Mostrar Tudo |
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Palavras-Chave: |
SRLV. |
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Thesaurus Nal: |
Genetic techniques and protocols; Goat diseases; Goats; Lentivirus; Sheep diseases. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218347/1/CNPC-2020-Art-40.pdf
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Marc: |
LEADER 02915naa a2200325 a 4500
001 2127175
005 2020-11-29
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0239916$2DOI
100 1 $aARAÚJO, J. F.
245 $aVertical transmissibility of small ruminant lentivirus.$h[electronic resource]
260 $c2020
520 $aAbstract: This study aimed to evaluate by means of Nested Polymerase Chain Reaction (nPCR), co-cultivation and sequencing, with genetic comparison between strains (mother/newborn), the occurrence of vertical transmission of Small Ruminant Lentiviruses (SRLV) from naturally occurring nannies infected for their offspring. For the detection of SRLV seropositive progenitors, blood was collected from 42 nannies in the final third of gestation in tubes with and without anticoagulant. The diagnostic tests used were Western Blot (WB) and nPCR. During the period of birth, the same blood collection procedure was performed on 73 newborns at zero hours of birth, with the same diagnostic tests. Seventeen blood samples from seven-day-old kids, proven positive for SRLV by nPCR, chosen at random, were subjected to coculture in goat synovial membrane (GSM) cells for 105 days. The pro-viral DNA extracted from the cell supernatant from the coculture was subjected to nPCR. For DNA sequencing from the nPCR products, nine positive samples were chosen at random, four nannies with their respective offspring, also positive. Each sample was performed in triplicate, thus generating 27 nPCR products of which only 19 were suitable for analysis. Among the 42 pregnant goats, in 50% (21/42) pro-viral DNA was detected by nPCR, while in the WB, only 7.14% (3/42) presented antibodies against SRLV. Regarding neonates, of the 73 kids, 34 (46.57%) were positive for the virus, using the nPCR technique, while in the serological test (WB), three positive animals (4.10%) were observed. The coculture of the 17 samples with a positive result in the nPCR was confirmed in viral isolation by amplification of the SRLV pro-viral DNA. When aligned, the pro-viral DNA sequences (nannies and their respective offspring) presented homology in relation to the standard strain CAEV Co. It was concluded that the transmission of SRLV through intrauterine route was potentially the source of infection in the newborn goats.
650 $aGenetic techniques and protocols
650 $aGoat diseases
650 $aGoats
650 $aLentivirus
650 $aSheep diseases
653 $aSRLV
700 1 $aANDRIOLI, A.
700 1 $aPINHEIRO, R. R.
700 1 $aSIDER, L. H.
700 1 $aSOUSA, A. L. M. de
700 1 $aAZEVEDO, D. A. A. de
700 1 $aPEIXOTO, R. M.
700 1 $aLIMA, A. M. C.
700 1 $aDAMASCENO, E. M.
700 1 $aSOUZA, S. C. R.
700 1 $aTEIXEIRA, M. F. da S.
773 $tPLoSONE$gv. 15, n. 11, e0239916, Nov. 2020.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 8 | |
| 1. |  | LARA, L. A. DE C.; SANTOS, M. F.; JANK, L.; CHIARI, L.; GARCIA, A. A. F. Modeling the variance-covariance matrix of genetic and residual effects in a Panicum maximum genome wide selection experiment. In: INTERNATIONAL CONFERENCE ON QUANTITATIVE GENETICS, 5., Madison, Wisconsin, USA, 2016. Proceedings... Madison, Wisconsin, USA: ICQG, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 2. | | FERREIRA, R. C. U.; LARA, L. A. de C.; CHIARI, L.; BARRIOS, S. C. L.; VALLE, C. B. do; GARCIA, A. A. F.; SOUZA, A. P. de. The first genotyping by sequencing in an interespecific population of Urochloa decumbens. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro: resumos das palestras. p. 104| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | LARA, L. A. de C.; SANTOS, J. P. R. dos; SANTOS, M. F.; CHIARI, L.; VILELA, M. de M.; JANK, L.; GARCIA, A. A. F. Predictive ability of G-Blup model in a tetraploid sexual population of Panicum maximum. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 93| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | LARA, L. A. de C.; SANTOS, M. F.; JANK, L.; CHIARI, L.; VILELA, M. de M.; AMADEU, R. R.; SANTOS, J. P. R. dos; SILVA, F. G. da; ZENG, Z.-B.; GARCIA, A. A. F. Genomic Selection with Allele Dosage in Panicum maximum Jacq. G3: Genes|Genomes|Genetics, v. 9, p. 2463-2475, August 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | LARA, L. A. de C.; CAVALCANTE, D. N. C.; DÉO, T. G.; SANTOS, M. F.; CHIARI, L.; JANK, L.; VILELA, M. de M.; ZENG, Z. B.; GARCIA, A. A. F. Genotipagem por sequenciamento e dosagem alélica em Panicum maximum. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 12., 2016, Campo Grande, MS. p. 54-55.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 6. | | FERREIRA, R. C. U.; LARA, L. A. de C.; CHIARI, L.; BARRIOS, S. C. L.; VALLE, C. B. do; VALERIO, J. R.; TORRES, F. Z. V.; GARCIA, A. A. F.; SOUZA, A. P. de. Genetic Mapping With Allele Dosage Information in Tetraploid Urochloa decumbens (Stapf) R. D. Webster Reveals Insights Into Spittlebug (Notozulia entreriana Berg) Resistance. Frontiers in Plant Science, v. 10, February, 2019.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. | | MORAES, A. da C. L.; MOLLINARI, M.; FERREIRA, R. C. U.; AONO, A.; LARA, L. A. de C.; PESSOA FILHO, M. A. C. de P.; BARRIOS, S. C. L.; GARCIA, A. A. F.; VALLE, C. B. do; SOUZA, A. P. de; VIGNA, B. B. Z. Advances in genomic characterization of Urochloa humidicola: exploring polyploid inheritance and apomixis. Theoretical and Applied Genetics, v. 136, n. 11, 2023. 17 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Gado de Corte; Embrapa Pecuária Sudeste. |
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| 8. | | AONO, A. H.; FERREIRA, R. C. U.; MORAES, A. da C. L.; LARA, L. A. de C.; PIMENTA, R. J. G.; COSTA, E. A.; PINTO, L. R.; LANDELL, M. G. de A.; SANTOS, M. F.; JANK, L.; BARRIOS, S. C. L.; VALLE, C. B.; CHIARI, L.; GARCIA, A. A. F.; KUROSHU, R. M.; LORENA, A. C.; GORJANC, G.; SOUZA, A. P. de. A joint learning approach for genomic prediction in polyploid grasses. Scientific Reports, 12, article 12499, 2022. 17 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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