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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/08/2020 |
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Data da última atualização: |
25/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARENA, G. D.; RAMOS-GONZALEZ, P. L.; FALK, B. W.; CASTEEL, C. L.; ASTUA, J. de F.; MACHADO, M. A. |
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Afiliação: |
GABRIELLA D. ARENA, Centro de Citricultura Sylvio Moreira; PEDRO LUIS RAMOS-GONZALEZ, Instituto Biológico; BRYCE W. FALK, University of California; CLARE L. CASTEEL, CASTEEL; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, Centro de Citricultura Sylvio Moreira. |
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Título: |
Plant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Plant Science, August, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses. MenosCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum st... Mostrar Tudo |
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Thesagro: |
Fruta Cítrica. |
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Categoria do assunto: |
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Marc: |
LEADER 03074naa a2200193 a 4500
001 2124551
005 2020-08-25
008 2020 bl uuuu u00u1 u #d
100 1 $aARENA, G. D.
245 $aPlant immune system activation upon Citrus Leprosis Virus c infection is mimicked by the ectopic expression of the P61 viral protein.$h[electronic resource]
260 $c2020
520 $aCitrus leprosis virus C (CiLV-C, genus Cilevirus, family Kitaviridae) is an atypical virus that does not spread systemically in its plant hosts. Upon its inoculation by Brevipalpus mites, only localized lesions occur, and the infection remains limited to cells around mite feeding sites. Here, we aimed to gain insights into the putative causes of viral unfitness in plants by expanding the limited knowledge of the molecular mechanisms underlying plant/kitavirid interactions. Firstly, we quantified the CiLV-C viral RNAs during the infection in Arabidopsis thaliana plants using RT-qPCR and systematized it by defining three stages of distinguishing subgenomic and genomic RNA accumulation: i) 0–24 h after infestation, ii) 2–4 days after infestation (dai), and iii) 6–10 dai. Accordingly, the global plant response to CiLV-C infection was assessed by RNA-Seq at each period. Results indicated a progressive reprogramming of the plant transcriptome in parallel to the increasing viral loads. Gene ontology enrichment analysis revealed the induction of cell growth-related processes at the early stages of the infection and the triggering of the SA-mediated pathway, ROS burst and hypersensitive response (HR) at the presymptomatic stage. Conversely, infected plants downregulated JA/ET-mediated pathways and processes involved in the primary metabolism including photosynthesis. Marker genes of unfolded protein response were also induced, suggesting a contribution of the endoplasmic reticulum stress to the cell death caused by the viral infection. Finally, we transiently expressed CiLV-C proteins in Nicotiana benthamiana plants to undertake their roles in the elicited plant responses. Expression of the CiLV-C P61 protein consistently triggered ROS burst, upregulated SA- and HR-related genes, increased SA levels, reduced JA levels, and caused cell death. Mimicry of responses typically observed during CiLV-C–plant interaction indicates P61 as a putative viral effector causing the HR-like symptoms associated with the infection. Our data strengthen the hypothesis that symptoms of CiLV-C infection might be the outcome of a hypersensitive-like response during an incompatible interaction. Consequently, the locally restricted infection of CiLV-C, commonly observed across infections by kitavirids, supports the thesis that these viruses, likely arising from an ancestral arthropod-infecting virus, are unable to fully circumvent plant defenses.
650 $aFruta Cítrica
700 1 $aRAMOS-GONZALEZ, P. L.
700 1 $aFALK, B. W.
700 1 $aCASTEEL, C. L.
700 1 $aASTUA, J. de F.
700 1 $aMACHADO, M. A.
773 $tFrontiers in Plant Science, August, 2020.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/10/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BRIGHENTI, A. M. |
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Afiliação: |
ALEXANDRE MAGNO B DOS SANTOS, CNPGL. |
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Título: |
Sulfentrazone for volunteer soybean control and selectivity in sunflower CROP. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Interciência, v. 43, n. 4, p. 256-260, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
SUMMARY The control of volunteer soybean (Glycine max) plants between crop seasons is mandatory due to the increasing incidence of diseases, mainly Asian soybean rust (Phakopsora pachyrhizi). Moreover, competition from volunteer soybean plants can cause yield losses in other crops. The objectives of this study were to evaluate the control of volunteer soybean plants by sulfentrazone doses and selectivity in sunflower crop (Helianthus annuus). Two experiments were carried out under field conditions in the municipality of Rio Verde, Goiás State, Brazil. The experimental design was randomized complete blocks with four replications. Treatments applied in Experiment 1 were sulfentrazone 0, 25, 50, 100 and 150g·ha-1 and hoed check. The same treatments were applied in Experiment 2 plus sulfentrazone 200 and 250g·ha-1. Polynomial regression models were fitted to the data of percentage of sunflower phytotoxicity, percentage of soybean control, sunflower plant height, head diameter, sunflower grain yield and dry biomass of volunteer soybean plants. Doses ranging from 114.1 to 158.8g·ha-1 provided the highest sunflower yield, preventing the competition of volunteer soybean plants with the crop. Sulfentrazone did not completely eliminate the volunteer soybean plants, but there was a temporary stoppage of soybean growth that enabled the initial startup of the sunflower plants. |
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Palavras-Chave: |
Spontaneous Soybean. |
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Thesagro: |
Glycine Max; Helianthus Annuus. |
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Thesaurus NAL: |
Sulfentrazone; Weeds. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01913naa a2200181 a 4500
001 2098031
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
100 1 $aBRIGHENTI, A. M.
245 $aSulfentrazone for volunteer soybean control and selectivity in sunflower CROP.$h[electronic resource]
260 $c2018
520 $aSUMMARY The control of volunteer soybean (Glycine max) plants between crop seasons is mandatory due to the increasing incidence of diseases, mainly Asian soybean rust (Phakopsora pachyrhizi). Moreover, competition from volunteer soybean plants can cause yield losses in other crops. The objectives of this study were to evaluate the control of volunteer soybean plants by sulfentrazone doses and selectivity in sunflower crop (Helianthus annuus). Two experiments were carried out under field conditions in the municipality of Rio Verde, Goiás State, Brazil. The experimental design was randomized complete blocks with four replications. Treatments applied in Experiment 1 were sulfentrazone 0, 25, 50, 100 and 150g·ha-1 and hoed check. The same treatments were applied in Experiment 2 plus sulfentrazone 200 and 250g·ha-1. Polynomial regression models were fitted to the data of percentage of sunflower phytotoxicity, percentage of soybean control, sunflower plant height, head diameter, sunflower grain yield and dry biomass of volunteer soybean plants. Doses ranging from 114.1 to 158.8g·ha-1 provided the highest sunflower yield, preventing the competition of volunteer soybean plants with the crop. Sulfentrazone did not completely eliminate the volunteer soybean plants, but there was a temporary stoppage of soybean growth that enabled the initial startup of the sunflower plants.
650 $aSulfentrazone
650 $aWeeds
650 $aGlycine Max
650 $aHelianthus Annuus
653 $aSpontaneous Soybean
773 $tInterciência$gv. 43, n. 4, p. 256-260, 2018.
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