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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/04/2018 |
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Data da última atualização: |
30/04/2018 |
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Autoria: |
CARVALHO, E. H.; MENDES, A. S.; TAKAHSHI, S. E.; ASSUMPÇÃO, R. A. B.; BONAMIGO, D. V.; MULLER, D.; SIKORSKI, R. R. |
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Afiliação: |
Erich H. Carvalho, Programa de Pós-Graduação em Zootecnia - PPGZO/Universidade Tecnológica Federal do Paraná - UTFPR; Angélica S. Mendes, Departamento de Agronomia/UTFPR; Sabrina E. Takahashi, Departamento de Zootecnia/UTFPR; Rosângela A. B. Assumpção, Departamento de Tecnologia Alimentar/UTFPR; Douglas V. Bonamigo, Departamento de Zootecnia/UTFPR; Daniel Müller, Departamento de Zootecnia/UTFPR; Rosana R. Sikorski, Departamento de Zootecnia/UTFPR. |
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Título: |
Defined and undefined commercial probiotics cultures in the prevention of Salmonella Enteritidis in broilers. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 2, p. 271-276 , fevereiro 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Probióticos comerciais de culturas definidas e indefinidas na prevenção de Salmonella Enteritidis em frangos de corte. |
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Conteúdo: |
This study aimed to evaluate the efficacy of probiotics from different formations, defined and undefined cultures, applied in the control of Salmonella Enteritidis in broilers, identifying the compositions and states for which the probiotics are more effective. For that, 390 broilers were inoculated orally with 1.00 ml of Salmonella Enteritidis at a concentration of 1.2x109 CFU (Colony Forming Units). The experimental design used was randomized blocks with 5 treatments and 6 replications, totaling 30 boxes with 13 birds/box (13 birds/m2). The treatments were provided via drinking water 1 hour after inoculation, keeping a daily treatment of 12 hours with probiotics, for 3 consecutive days (birds at 1, 2 and 3 days of age). In general, the five treatments conducted were: T1 - Control without probiotic, T2 - Probiotic A (defined culture - lyophilized form, strain 7), T3 - Probiotic B (defined culture - lyophilized form, strain 11), T4 - Probiotic C (undefined culture liquid form), T5 - Probiotic D (undefined culture - liquid form). After treatments, performance was evaluated through average body weight, feed conversion and mortality counting. Microbiological analysis and Salmonella isolation were performed using MPN (Most Probable Number) and selective enrichment technique methods, respectively. Samples of ileum and liver pool, cecal tonsils, cecum, heart and spleen pool were collected at 5 and 31 days of age. No differences were observed on growth performance and isolation of Salmonella Enteritidis (p≥0.05). All probiotics applied were effective on reducing Salmonella Enteritidis colonization in the ileum, cecal tonsils, and cecum at 5 days of life. Probiotics T2 and T5 has shown effectiveness in reducing colonization at 31 days, being considered the most efficient on Salmonella Enteritidis control, for the intestines segments evaluated. It was not possible to affirm which probiotics formation, defined or undefined, is more efficient for Salmonella Enteritidis control. MenosThis study aimed to evaluate the efficacy of probiotics from different formations, defined and undefined cultures, applied in the control of Salmonella Enteritidis in broilers, identifying the compositions and states for which the probiotics are more effective. For that, 390 broilers were inoculated orally with 1.00 ml of Salmonella Enteritidis at a concentration of 1.2x109 CFU (Colony Forming Units). The experimental design used was randomized blocks with 5 treatments and 6 replications, totaling 30 boxes with 13 birds/box (13 birds/m2). The treatments were provided via drinking water 1 hour after inoculation, keeping a daily treatment of 12 hours with probiotics, for 3 consecutive days (birds at 1, 2 and 3 days of age). In general, the five treatments conducted were: T1 - Control without probiotic, T2 - Probiotic A (defined culture - lyophilized form, strain 7), T3 - Probiotic B (defined culture - lyophilized form, strain 11), T4 - Probiotic C (undefined culture liquid form), T5 - Probiotic D (undefined culture - liquid form). After treatments, performance was evaluated through average body weight, feed conversion and mortality counting. Microbiological analysis and Salmonella isolation were performed using MPN (Most Probable Number) and selective enrichment technique methods, respectively. Samples of ileum and liver pool, cecal tonsils, cecum, heart and spleen pool were collected at 5 and 31 days of age. No differences were observed on growth performance and isolation of ... Mostrar Tudo |
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Palavras-Chave: |
Bird health; Frangos de corte; Probióticos comerciais. |
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Thesagro: |
Bacteriose; Segurança alimentar. |
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Thesaurus Nal: |
Bacterial infections; Broiler chickens; Probiotics; Salmonella Enteritidis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/176163/1/Defined-and-undefined-commercial-probiotics-cultures.pdf
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Marc: |
LEADER 03108naa a2200313 a 4500
001 2091059
005 2018-04-30
008 2018 bl uuuu u00u1 u #d
100 1 $aCARVALHO, E. H.
245 $aDefined and undefined commercial probiotics cultures in the prevention of Salmonella Enteritidis in broilers.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Probióticos comerciais de culturas definidas e indefinidas na prevenção de Salmonella Enteritidis em frangos de corte.
520 $aThis study aimed to evaluate the efficacy of probiotics from different formations, defined and undefined cultures, applied in the control of Salmonella Enteritidis in broilers, identifying the compositions and states for which the probiotics are more effective. For that, 390 broilers were inoculated orally with 1.00 ml of Salmonella Enteritidis at a concentration of 1.2x109 CFU (Colony Forming Units). The experimental design used was randomized blocks with 5 treatments and 6 replications, totaling 30 boxes with 13 birds/box (13 birds/m2). The treatments were provided via drinking water 1 hour after inoculation, keeping a daily treatment of 12 hours with probiotics, for 3 consecutive days (birds at 1, 2 and 3 days of age). In general, the five treatments conducted were: T1 - Control without probiotic, T2 - Probiotic A (defined culture - lyophilized form, strain 7), T3 - Probiotic B (defined culture - lyophilized form, strain 11), T4 - Probiotic C (undefined culture liquid form), T5 - Probiotic D (undefined culture - liquid form). After treatments, performance was evaluated through average body weight, feed conversion and mortality counting. Microbiological analysis and Salmonella isolation were performed using MPN (Most Probable Number) and selective enrichment technique methods, respectively. Samples of ileum and liver pool, cecal tonsils, cecum, heart and spleen pool were collected at 5 and 31 days of age. No differences were observed on growth performance and isolation of Salmonella Enteritidis (p≥0.05). All probiotics applied were effective on reducing Salmonella Enteritidis colonization in the ileum, cecal tonsils, and cecum at 5 days of life. Probiotics T2 and T5 has shown effectiveness in reducing colonization at 31 days, being considered the most efficient on Salmonella Enteritidis control, for the intestines segments evaluated. It was not possible to affirm which probiotics formation, defined or undefined, is more efficient for Salmonella Enteritidis control.
650 $aBacterial infections
650 $aBroiler chickens
650 $aProbiotics
650 $aSalmonella Enteritidis
650 $aBacteriose
650 $aSegurança alimentar
653 $aBird health
653 $aFrangos de corte
653 $aProbióticos comerciais
700 1 $aMENDES, A. S.
700 1 $aTAKAHSHI, S. E.
700 1 $aASSUMPÇÃO, R. A. B.
700 1 $aBONAMIGO, D. V.
700 1 $aMULLER, D.
700 1 $aSIKORSKI, R. R.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 2, p. 271-276 , fevereiro 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
29/09/2016 |
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Data da última atualização: |
24/11/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
QUADROS, A. F. F.; BOARI, A. de J.; BATISTA, I. C. A. |
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Afiliação: |
Ayane Fernanda Ferreira Quadros, GRADUANDA UFRA; ALESSANDRA DE JESUS BOARI, CPATU; Izabel Cristina Alves Batista, GRADUANDA UFRA. |
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Título: |
Detecção de Cucumber mosaic virus do subgrupo- IB em feijão-de-metro no estado do Pará. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 20.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 4., 2016, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2016. |
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Páginas: |
p. 31-34. |
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Idioma: |
Português |
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Conteúdo: |
O feijão-de-metro é uma hortaliça amplamente cultivada nos municípios da região metropolitana de Belém. Diversas doenças podem comprometer a sua produtividade, dentre elas as viroses. Recentemente, foi detectado o Cucumber mosaic virus (CMV) em vagens de feijão-de-metro provenientes do município de Castanhal-PA. Este trabalho teve como objetivo identificar o subgrupo do CMV detectado em vagens de feijão-de-metro, por meio de RT-PCR, sequenciamento do ácido nucléico e análise utilizando o programa Blast, ClustalW e MEGA 7.0. Para isso, foi feita a extração de ácidos nucleicos total a partir de folhas de fumo inoculado com o isolado. Posteriormente, foi realizado o RT-PCR utilizando os primers específicos (CMV-CPR e CMV-CPF). A partir da análise da filogenia foi observado que o isolado formou um clado com os acessos do subgrupo IB de CMV. |
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Palavras-Chave: |
CMV; Subgrupo; Vigna unguiculata ssp unguiculata. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147971/1/Pibic2016-p31.pdf
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Marc: |
LEADER 01557nam a2200181 a 4500
001 2053750
005 2016-11-24
008 2016 bl uuuu u00u1 u #d
100 1 $aQUADROS, A. F. F.
245 $aDetecção de Cucumber mosaic virus do subgrupo- IB em feijão-de-metro no estado do Pará.$h[electronic resource]
260 $aIn: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 20.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 4., 2016, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental$c2016
300 $ap. 31-34.
520 $aO feijão-de-metro é uma hortaliça amplamente cultivada nos municípios da região metropolitana de Belém. Diversas doenças podem comprometer a sua produtividade, dentre elas as viroses. Recentemente, foi detectado o Cucumber mosaic virus (CMV) em vagens de feijão-de-metro provenientes do município de Castanhal-PA. Este trabalho teve como objetivo identificar o subgrupo do CMV detectado em vagens de feijão-de-metro, por meio de RT-PCR, sequenciamento do ácido nucléico e análise utilizando o programa Blast, ClustalW e MEGA 7.0. Para isso, foi feita a extração de ácidos nucleicos total a partir de folhas de fumo inoculado com o isolado. Posteriormente, foi realizado o RT-PCR utilizando os primers específicos (CMV-CPR e CMV-CPF). A partir da análise da filogenia foi observado que o isolado formou um clado com os acessos do subgrupo IB de CMV.
653 $aCMV
653 $aSubgrupo
653 $aVigna unguiculata ssp unguiculata
700 1 $aBOARI, A. de J.
700 1 $aBATISTA, I. C. A.
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Embrapa Amazônia Oriental (CPATU) |
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