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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
04/12/2015 |
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Data da última atualização: |
14/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARQUES, M. G.; BARROS, F. R. O. de; GOISSIS, M. D.; GIASSETTI, M. I.; ASSUMPÇÃO, M. E. O. D.; VISINTIN, J. A. |
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Afiliação: |
MARIANA GROKE MARQUES, CNPSA; FLAVIA REGINA OLIVEIRA DE BARROS, USP; MARCELO DEMARCHI GOISSIS, USP; MARIANA IANELLO GIASSETTI, USP; MAYRA ELENA ORTIZ D'AVILA ASSUMPÇÃO, USP; JOSÉ ANTONIO VISINTIN, USP. |
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Título: |
Effect of Oocyte recovery techniques on in vitro production of swine embryos. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Open Journal of Animal Sciences, v. 5, p. 467-473, 2015. |
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DOI: |
http://dx.doi.org/10.4236/ojas.2015.54048 |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro production of swine embryos is a valuable tool to generate clones and genetically modified pigs during a short period of time. However, the efficiency of the existing methods is extremely low and the oocyte quality and quantity represent important obstacles on the success of in vitro production of embryos. Therefore, the aim of this study was to compare the in vitro maturation, fertilization and subsequent embryo development rates of oocytes recovered by ovary slicing or follicular aspiration. The oocyte recovery rate (grade 1 COC/ovary) was higher (p = 0.0083) in the slicing group when compared to the aspiration group. No differences were observed between groups regarding in vitro maturation and early cleavage rates. A higher percentage of oocytes recovered by follicular aspiration reached the blastocyst stage after IVF when compared to the ovary slicing method (p = 0.0395). However, no difference on blastocyst cell number was observed. Although the recovery of oocytes using the slicing technique yielded more grade 1 oocytes per ovary than the aspiration method, the number of oocytes that reached the blastocyst stage after IVF by the slicing method was lower when compared with oocytes obtained by aspiration, as observed by lower blastocyst rates. In conclusion, the follicular aspiration is the method of choice for porcine in vitro embryo production. |
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Palavras-Chave: |
Embryo(animal); Inceminação artificial. |
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Thesagro: |
Embrião animal; Reprodução animal; Suíno. |
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Thesaurus Nal: |
Animal reproduction; Oocytes; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02243naa a2200289 a 4500
001 2030672
005 2016-03-14
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4236/ojas.2015.54048$2DOI
100 1 $aMARQUES, M. G.
245 $aEffect of Oocyte recovery techniques on in vitro production of swine embryos.$h[electronic resource]
260 $c2015
520 $aIn vitro production of swine embryos is a valuable tool to generate clones and genetically modified pigs during a short period of time. However, the efficiency of the existing methods is extremely low and the oocyte quality and quantity represent important obstacles on the success of in vitro production of embryos. Therefore, the aim of this study was to compare the in vitro maturation, fertilization and subsequent embryo development rates of oocytes recovered by ovary slicing or follicular aspiration. The oocyte recovery rate (grade 1 COC/ovary) was higher (p = 0.0083) in the slicing group when compared to the aspiration group. No differences were observed between groups regarding in vitro maturation and early cleavage rates. A higher percentage of oocytes recovered by follicular aspiration reached the blastocyst stage after IVF when compared to the ovary slicing method (p = 0.0395). However, no difference on blastocyst cell number was observed. Although the recovery of oocytes using the slicing technique yielded more grade 1 oocytes per ovary than the aspiration method, the number of oocytes that reached the blastocyst stage after IVF by the slicing method was lower when compared with oocytes obtained by aspiration, as observed by lower blastocyst rates. In conclusion, the follicular aspiration is the method of choice for porcine in vitro embryo production.
650 $aAnimal reproduction
650 $aOocytes
650 $aSwine
650 $aEmbrião animal
650 $aReprodução animal
650 $aSuíno
653 $aEmbryo(animal)
653 $aInceminação artificial
700 1 $aBARROS, F. R. O. de
700 1 $aGOISSIS, M. D.
700 1 $aGIASSETTI, M. I.
700 1 $aASSUMPÇÃO, M. E. O. D.
700 1 $aVISINTIN, J. A.
773 $tOpen Journal of Animal Sciences$gv. 5, p. 467-473, 2015.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 5 | |
| 2. |  | LIMA, A. S. de; MARQUES, M. G.; NASCIMENTO, A. B.; ASSUMPÇÃO, M. E. O. D.; VISITIN, J. A. Effect of hormonal supplementation periods and in vitro maturation media on developmental competence of pig oocytes. Acta Scientiae Veterinariae, Porto Alegre, v. 38, n. 4, p. 407-413, 2010. Disponível em: . Acesso em: 24 fev. 2011. Projeto/Plano de Ação: 16.00.300.04-00.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | SOTO, F. R. M.; VUADEN, E. R.; COELHO, C, de P.; BONAMIN, L. V.; AZEVEDO, S. S. dos; BENITES, N. R.; BARROS, F. R. O. de; GOISSIS, M. D.; ASSUMPÇÃO, M. E. O. D.; VISITIN, J. A.; MARQUES, M. G. Effects of utilization of homeopathic elements in commercial diluent on swine sperm viability. Vitro Cellular & Developmental Biology - Animal, v. 47, n. 3, p. 205-209, 2011. Projeto: 11.11.11.111.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 5 | |
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