Data/hora: 24/06/2024 18:57:28

Biblioteca(s): | Embrapa Pesca e Aquicultura.
Data corrente: | 03/03/2015
Data da última atualização: | 10/04/2018
Autoria: | LADINIG, A.; LUNNEY, J. K.; SOUZA, C. J. H.; ASHLEY, C.; PLASTOW, G.; HARDING, J. C. S.
Afiliação: | ANDREA LADINIG, UNIVERSITY OF SASKATCHEWAN, CANADA; JOAN K. LUNNEY, USDA; CARLOS JOSE HOFF DE SOUZA, CNPASA; CAROLYN ASHLEY, UNIVERSITY OF SASKATCHEWAN, CANADA; GRAHAM PLASTOW, UNIVERSITY OF ALBERTA, CANADA; JOHN C. S. HARDING, UNIVERSITY OF SASKATCHEWAN, CANADA.
Título: | Cytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome.
Ano de publicação: | 2014
Fonte/Imprenta: | Veterinary Research, v. 45, 2014.
DOI: | 10.1186/s13567-014-0113-8
Idioma: | Inglês
Conteúdo: | In spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1&#946;), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFN&#945;) and interferon gamma (IFN&#947;). Three cytokines (IFN&#945;, CCL2, IFN&#947;) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFN&#945; were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFN&#945;, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFN&#945;, CCL2, and IFN&#947; in serum, and IFN&#947; in supernatants of PMA/Iono stimulated PBMC were significantly associated with viral load in tissues, serum or both. However, only IFN&#945; in supernatants of PRRSv stimulated PBMC was significantly associated with fetal mortality rate. We conclude that of the eight cytokines tested in this study IFN&#945; was the best indicator of viral load and severity of reproductive PRRSv infection.
Thesagro: | Porco; Reprodução animal; Respiração; Suino; Virus.
Thesaurus Nal: | Cytokines; Porcine reproductive and respiratory syndrome virus; Swine.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/119182/1/cnpasa.pdf
Registro original: | Embrapa Pesca e Aquicultura (CNPASA)
Exemplares: | CNPASA - (235-1) - UPC - AP - DD - CNPASA-SP54 -  - 2014.054 - DIS