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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
28/04/2023 |
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Data da última atualização: |
28/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARRIEL-ELIAS, M. T.; PEREIRA, A. K.; ARRIEL, G. C. T. F.; ALEXANDRE, G.; BEZERRA, G. de A.; FILL, T. P.; SEVERINO, V. G. P.; FILIPPI, M. C. C. de. |
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Afiliação: |
MARINA TEIXEIRA ARRIEL-ELIAS, UFG; ALANA KELYENE PEREIRA, UNICAMP; GABRIEL CARLOS T. F. ARRIEL, UniEVANGÉLICA; GERSO ALEXANDRE, UFG; GUSTAVO DE ANDRADE BEZERRA, UFG; TAÍCIA PACHECO FILL, UNICAMP; VANESSA GISELE PASQUALOTTO SEVERINO, UFG; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
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Título: |
Molecular networking as a tool to annotate the metabolites of Bacillus sp. and Serratia marcescens isolates and evaluate their fungicidal effects against Magnapothe oryzae and Bipolaris oryzae. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
3 Biotech, v. 13, n. 5, 148, May 2023. |
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ISSN: |
2190-5738 |
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DOI: |
https://doi.org/10.1007/s13205-023-03547-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobacteria are valuable sources of compounds that can be used for the integrated management of diseases in rice. Here, we aimed to explore the metabolism and organize and annotate the metabolites of Bacillus sp. and Serratia marcescens isolates using molecular networking and evaluate their fungicidal effects against Magnaporthe oryzae and Bipolaris oryzae. We obtained bacterial extracts after 6 and 16-h incubation via liquid?liquid extraction using ethyl acetate as solvent. We performed UHPLC-MS analysis and data processing using molecular networking and conducted biological assays in rice plants. Using the Global Natural Product Social spectral libraries, we annotated the following compounds: austinoneol, Phe-Pro, N-acetyl-l-leucine, Leu-Gly, Ile-Leu, Phe-Pro, 2,5-piperazinedione, 3-(1H-indol-3-methyl)-6-methyl-cyclo(d-Trp-l-Pro), and cholic acid. Results of the biological assays showed that the bacterial extracts reduced the mycelial growth of both pathogens in all treatments compared to the control. In the greenhouse setup, 8 days after the challenge for leaf gray spot and leaf blast, all treatments affected up to 4.4% of the leaf area, with an area under disease progress curve of 13.24, showing significant difference compared to the control, which affected 23% of the leaf area, with an AUDPC of 44.65. Our study provides potential new sources of natural products to be applied in the integrated management of rice. |
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Palavras-Chave: |
Molecules; Natural products. |
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Thesagro: |
Arroz; Bactéria; Marcador Molecular. |
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Thesaurus Nal: |
Bacillus (bacteria); Bipolaris oryzae; Magnaporthe oryzae; Molecular biology; Rice; Serratia marcescens. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02584naa a2200361 a 4500
001 2153438
005 2023-04-28
008 2023 bl uuuu u00u1 u #d
022 $a2190-5738
024 7 $ahttps://doi.org/10.1007/s13205-023-03547-6$2DOI
100 1 $aARRIEL-ELIAS, M. T.
245 $aMolecular networking as a tool to annotate the metabolites of Bacillus sp. and Serratia marcescens isolates and evaluate their fungicidal effects against Magnapothe oryzae and Bipolaris oryzae.$h[electronic resource]
260 $c2023
520 $aRhizobacteria are valuable sources of compounds that can be used for the integrated management of diseases in rice. Here, we aimed to explore the metabolism and organize and annotate the metabolites of Bacillus sp. and Serratia marcescens isolates using molecular networking and evaluate their fungicidal effects against Magnaporthe oryzae and Bipolaris oryzae. We obtained bacterial extracts after 6 and 16-h incubation via liquid?liquid extraction using ethyl acetate as solvent. We performed UHPLC-MS analysis and data processing using molecular networking and conducted biological assays in rice plants. Using the Global Natural Product Social spectral libraries, we annotated the following compounds: austinoneol, Phe-Pro, N-acetyl-l-leucine, Leu-Gly, Ile-Leu, Phe-Pro, 2,5-piperazinedione, 3-(1H-indol-3-methyl)-6-methyl-cyclo(d-Trp-l-Pro), and cholic acid. Results of the biological assays showed that the bacterial extracts reduced the mycelial growth of both pathogens in all treatments compared to the control. In the greenhouse setup, 8 days after the challenge for leaf gray spot and leaf blast, all treatments affected up to 4.4% of the leaf area, with an area under disease progress curve of 13.24, showing significant difference compared to the control, which affected 23% of the leaf area, with an AUDPC of 44.65. Our study provides potential new sources of natural products to be applied in the integrated management of rice.
650 $aBacillus (bacteria)
650 $aBipolaris oryzae
650 $aMagnaporthe oryzae
650 $aMolecular biology
650 $aRice
650 $aSerratia marcescens
650 $aArroz
650 $aBactéria
650 $aMarcador Molecular
653 $aMolecules
653 $aNatural products
700 1 $aPEREIRA, A. K.
700 1 $aARRIEL, G. C. T. F.
700 1 $aALEXANDRE, G.
700 1 $aBEZERRA, G. de A.
700 1 $aFILL, T. P.
700 1 $aSEVERINO, V. G. P.
700 1 $aFILIPPI, M. C. C. de
773 $t3 Biotech$gv. 13, n. 5, 148, May 2023.
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Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 4 | |
| 2. |  | DE VILLA, F. Z.; ECKERT, C.; NICKEL, O.; FAJARDO, T. V. M. Avaliação de longo prazo do efeito de eliminação de Apple stem grooving virus por quimioterapia em macieira cv. Royal Gala. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 12., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 8., 2014, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2014. p. 12| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 4. | | JUNKES, C. F. de O.; NICKEL, O.; ECKERT, C.; FAJARDO, T. V. M.; VANNI, M. F.; ARAGAO, F. J. L.; DANTAS, A. C. de M.; PETERS, J. A. Clonagem de construto de RNA interferente para produção de Maruba-Kaido resistente a vírus de macieira. In: SIMPÓSIO BRAILEIRO DE MICROBIOLOGIA APLICADA, 6.; ENCONTRO LATINO-AMERICANO DE MICROBIOLOGIA APLICADA, 2., 2012, Porto Alegre. Anais... Porto Alegre: UFRGS, 2012. p. 14-19. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 4 | |
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