Data/hora: 16/06/2024 20:46:05

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 31/10/2017
Data da última atualização: | 13/12/2017
Autoria: | ROSSI, G. B.; VALENTIM-NETO, P. A.; BLANK, M.; FARIA, J. C. de; ARISI, A. C. M.
Afiliação: | GABRIELA BARBOSA ROSSI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; PEDRO ALEXANDRE VALENTIM-NETO, UNIVERSIDADE FEDERAL DE SANTA CATARINA; MARTINA BLANK, UNIVERSIDADE FEDERAL DE SANTA CATARINA; JOSIAS CORREA DE FARIA, CNPAF; ANA CAROLINA MAISONNAVE ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA.
Título: | Comparison of grain proteome profiles of four brazilian common bean (Phaseolus vulgaris L.) cultivars.
Ano de publicação: | 2017
Fonte/Imprenta: | Journal of Agricultural and Food Chemistry, v. 65, n. 34, p. 7588-7597, Aug. 2017.
ISSN: | 0021-8561
DOI: | 10.1021/acs.jafc.7b03220
Idioma: | Inglês
Conteúdo: | Common bean (Phaseolus vulgaris L.) is a source of proteins for about one billion people worldwide. In Brazil, ?BRS Sublime?, ?BRS Vereda?, ?BRS Esteio?, and ?BRS Estilo? cultivars were developed by Embrapa to offer high yield to farmers and excellent quality to final consumers. In this work, grain proteomes of these common bean cultivars were compared based on two-dimensional gel electrophoresis (2-DE) and tandem mass spectrometry (MS/MS). Principal component analysis (PCA) was applied to compare 349 matched spots in these cultivars proteomes, and all cultivars were clearly separated in PCA plot. Thirty two differentially accumulated proteins were identified by MS. Storage proteins such as phaseolins, legumins, and lectins were the most abundant, and novel proteins were also identified. We have built a useful platform that could be used to analyze other Brazilian cultivars and genotypes of common beans.
Palavras-Chave: | 2-DE; PCA.
Thesagro: | Feijão; Phaseolus vulgaris; Proteína.
Thesaurus Nal: | Beans; Cultivars; Proteins; Proteome.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (34849-1) - UPC - AP - DD - 2017 -  - 2017 - DIS