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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/03/2011 |
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Data da última atualização: |
09/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARCURI, E. F.; ÂNGELO, F. F.; TALON, R.; BORGES, M. de F.; LEROY, S.; LOISEAU, G.; LANGE, C. C.; ANDRADE, N. J. de; MONTET, D. |
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Afiliação: |
EDNA FROEDER ARCURI, CNPGL; FABÍOLA FONSECA ÂNGELO, UFV; RÉGINE TALON, Institut National de la Recherche Agronomique; MARIA DE FATIMA BORGES, CNPAT; SABINE LEROY, Institut National de la Recherche Agronomique; GÉRARD LOISEAU, Centre de Coopération Internationale en Recherche Agronomique pour le Dévelopment; CARLA CHRISTINE LANGE, CNPGL; NÉLIO JOSÉ DE ANDRADE, UFV; DIDIER MONTET, Céntre de Coopération Internationale en Recherche Agronomique pour le Dévelopment. |
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Título: |
Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Food Protection, v. 73, n. 12, p. 2225-2231, 2010. |
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DOI: |
https://doi.org/10.4315/0362-028X-73.12.2225 |
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Idioma: |
Inglês |
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Conteúdo: |
A group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety. MenosA group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high ge... Mostrar Tudo |
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Palavras-Chave: |
Minas frescal cheese. |
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Thesagro: |
Staphylococcus Aureus. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/881434/1/Toxigenic-status-of-Staphylococcus-aureus-isolated-from-bovine.pdf
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Marc: |
LEADER 02530naa a2200253 a 4500
001 1881434
005 2022-08-09
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4315/0362-028X-73.12.2225$2DOI
100 1 $aARCURI, E. F.
245 $aToxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil.$h[electronic resource]
260 $c2010
520 $aA group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety.
650 $aStaphylococcus Aureus
653 $aMinas frescal cheese
700 1 $aÂNGELO, F. F.
700 1 $aTALON, R.
700 1 $aBORGES, M. de F.
700 1 $aLEROY, S.
700 1 $aLOISEAU, G.
700 1 $aLANGE, C. C.
700 1 $aANDRADE, N. J. de
700 1 $aMONTET, D.
773 $tJournal of Food Protection$gv. 73, n. 12, p. 2225-2231, 2010.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
20/06/1994 |
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Data da última atualização: |
19/05/2020 |
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Autoria: |
KURIHARA, C.; GOMES, G. C.; NORONHA, J. F. de. |
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Afiliação: |
Pesquisador DCO/Embrapa; Pesquisador DCO/Embrapa. |
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Título: |
Analise da rentabilidade economica da producao de hortalicas do Distrito Federal. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Revista de Economia e Sociologia Rural, Brasilia, v.28, n.4, p.123-132, 1990. |
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Idioma: |
Português |
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Palavras-Chave: |
Brasil; Defensivo agricola; Distrito Federal; Educational grants; Escolaridade; Farm equipments; Mao de obra; Production; Rentabilidade economica. |
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Thesagro: |
Adubo; Agricultor; Agricultura; Cerrado; Equipamento; Hortaliça; Maquina; Produção. |
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Thesaurus NAL: |
agriculture; crop yield; farmers; fertilizers; vegetables. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213073/1/analise-rentabilidade-economica.pdf
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Marc: |
LEADER 01050naa a2200397 a 4500
001 1112314
005 2020-05-19
008 1990 bl uuuu u00u1 u #d
100 1 $aKURIHARA, C.
245 $aAnalise da rentabilidade economica da producao de hortalicas do Distrito Federal.
260 $c1990
650 $aagriculture
650 $acrop yield
650 $afarmers
650 $afertilizers
650 $avegetables
650 $aAdubo
650 $aAgricultor
650 $aAgricultura
650 $aCerrado
650 $aEquipamento
650 $aHortaliça
650 $aMaquina
650 $aProdução
653 $aBrasil
653 $aDefensivo agricola
653 $aDistrito Federal
653 $aEducational grants
653 $aEscolaridade
653 $aFarm equipments
653 $aMao de obra
653 $aProduction
653 $aRentabilidade economica
700 1 $aGOMES, G. C.
700 1 $aNORONHA, J. F. de
773 $tRevista de Economia e Sociologia Rural, Brasilia$gv.28, n.4, p.123-132, 1990.
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Embrapa Unidades Centrais (AI-SEDE) |
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