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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
16/11/2017 |
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Data da última atualização: |
16/11/2017 |
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Autoria: |
ARCHER, J. F.; BEVERLEY, J. K.; WATSON, W. A. |
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Título: |
A field trial of the fluorescence antibody test for toxoplasmosis in the diagnosis of ovine abortion. |
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Ano de publicação: |
1971 |
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Fonte/Imprenta: |
The Veterinary Record, v. 88, n. 8, p. 206-208, Feb. 1971. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Abortion; Brain diseases; Diagnosis; Immunofluorescence. |
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Thesagro: |
Placenta. |
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Thesaurus Nal: |
Animal diseases; Immunology; Mice; Pregnancy complications; Sheep; Spleen; Toxoplasmosis. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 00765naa a2200277 a 4500
001 2079801
005 2017-11-16
008 1971 bl uuuu u00u1 u #d
100 1 $aARCHER, J. F.
245 $aA field trial of the fluorescence antibody test for toxoplasmosis in the diagnosis of ovine abortion.
260 $c1971
650 $aAnimal diseases
650 $aImmunology
650 $aMice
650 $aPregnancy complications
650 $aSheep
650 $aSpleen
650 $aToxoplasmosis
650 $aPlacenta
653 $aAbortion
653 $aBrain diseases
653 $aDiagnosis
653 $aImmunofluorescence
700 1 $aBEVERLEY, J. K.
700 1 $aWATSON, W. A.
773 $tThe Veterinary Record$gv. 88, n. 8, p. 206-208, Feb. 1971.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
27/08/2014 |
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Data da última atualização: |
02/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FALAVIGNA, V. da S.; PORTO, D. D.; BUFFON, V.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. |
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Afiliação: |
Vítor da Silveira Falavigna; Diogo Denardi Porto, CNPUV; Vanessa Buffon, CNPUV; Márcia Margis-Pinheiro; Giancarlo Pasquali; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Differential transcriptional profiles of dormancy-related genes in apple buds |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 32, p. 796-813, 2014. |
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Idioma: |
Inglês |
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Notas: |
DOI 10.1007/s11105-013-0690-0 |
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Conteúdo: |
The production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees. MenosThe production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts codin... Mostrar Tudo |
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Palavras-Chave: |
Apple; Castel Gala; Fuji Standard; Royal Gala. |
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Thesagro: |
Dormencia; Genetica vegetal; Maçã; Temperatura. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107373/1/Falavigna2014-Genes-Apple-Buds.pdf
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Marc: |
LEADER 02604naa a2200289 a 4500
001 1993551
005 2019-04-02
008 2014 bl uuuu u00u1 u #d
100 1 $aFALAVIGNA, V. da S.
245 $aDifferential transcriptional profiles of dormancy-related genes in apple buds$h[electronic resource]
260 $c2014
500 $aDOI 10.1007/s11105-013-0690-0
520 $aThe production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees.
650 $aDormencia
650 $aGenetica vegetal
650 $aMaçã
650 $aTemperatura
653 $aApple
653 $aCastel Gala
653 $aFuji Standard
653 $aRoyal Gala
700 1 $aPORTO, D. D.
700 1 $aBUFFON, V.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aPASQUALI, G.
700 1 $aREVERS, L. F.
773 $tPlant Molecular Biology Reporter$gv. 32, p. 796-813, 2014.
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Embrapa Uva e Vinho (CNPUV) |
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