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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
07/05/2013 |
Data da última atualização: |
17/01/2024 |
Autoria: |
LIMA-VERDE, I. B.; ROSSETTO, R.; MATOS, M. H. T.; CELESTINO, J. J. H.; BUENO, J. B.; SILVA, C. M. G.; FAUSTINO, L. R.; MORORÓ, M. B. S.; ARAÚJO, V. T.; CAMPELLO, C. C.; FIGUEIREDO, J. R. |
Título: |
Androstenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Animal Reproduction, Belo Horizonte, v. 7, n. 2, p. 80-89, Apr./Jun. 2010. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition. MenosAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of andr... Mostrar Tudo |
Palavras-Chave: |
Esteroidogenese; Foliculo pre-antral; FSH. |
Thesagro: |
Caprino; Cultura in vitro; Endocrinologia; Hormônio; Ovário; Reprodução animal. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03032naa a2200349 a 4500 001 1957239 005 2024-01-17 008 2010 bl uuuu u00u1 u #d 100 1 $aLIMA-VERDE, I. B. 245 $aAndrostenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro.$h[electronic resource] 260 $c2010 520 $aAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition. 650 $aCaprino 650 $aCultura in vitro 650 $aEndocrinologia 650 $aHormônio 650 $aOvário 650 $aReprodução animal 653 $aEsteroidogenese 653 $aFoliculo pre-antral 653 $aFSH 700 1 $aROSSETTO, R. 700 1 $aMATOS, M. H. T. 700 1 $aCELESTINO, J. J. H. 700 1 $aBUENO, J. B. 700 1 $aSILVA, C. M. G. 700 1 $aFAUSTINO, L. R. 700 1 $aMORORÓ, M. B. S. 700 1 $aARAÚJO, V. T. 700 1 $aCAMPELLO, C. C. 700 1 $aFIGUEIREDO, J. R. 773 $tAnimal Reproduction, Belo Horizonte$gv. 7, n. 2, p. 80-89, Apr./Jun. 2010.
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201. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ARAÚJO, V. L. V. P. de; LIRA JUNIOR, M. A.; SOUZA JÚNIOR, V. S. de; ARAUJO FILHO, J. C. de; FRACETTO, F. J. C.; ANDREOTE, F. D.; PEREIRA, A. P. de A.; MENDES JÚNIOR, J. P.; BARROS, F. M. do R.; FRACETTO, G. G. M. Bacteria from tropical semiarid temporary ponds promote maize growth under hydric stress. Microbiological Research, v. 240, 126564, Nov. 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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