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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Arroz e Feijão. |
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Data corrente: |
02/09/1993 |
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Data da última atualização: |
18/07/1996 |
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Autoria: |
ARAUJO, R. S. |
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Título: |
Mutational analysis of the cell surface and nodulation competitiveness of Rhizobium leguminosarum biovar phaseoli. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Madison: University of Wisconsin, 1993. |
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Páginas: |
118p. |
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Série: |
Tese de Doutorado. |
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Idioma: |
Inglês |
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Notas: |
Tese de Doutorado. Orientada por: Jo Handelsman. |
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Conteúdo: |
The use of Rhizobium inoculants for legumes is restricted by the poor nodulation competitiveness of many inoculant strains. In soil with high indiginous populations of bacteria that can nodulate a given host, the inoculant strains may be found in few or none of the nodules.This presents a problem when the indigenous strains fix little or no nitrogen and therefore do not provide a benefit to host. A strain of Rhizobium is a successful competitor when its representation in the nodules is greater than its representation in the inoculum. To determine whetherthe surface plays a role in nodulation competitiveness of Rhizobium leguminosarum bv.phaseoli (Rlp), I performed an extensive search and analysis of transposon-insertion mutants with altered cell surfaces derived from two nonisogenic parental strains of Rlp, KIM5s and CE3. I isolated and characterized one mutant of CE3, designated CE3003, with a domed colony morphology and a highly hydrophobic cell surface. On bean plants CE3003 was as efficient as CE3 in nodulation and acetylene reduction. The parent and the mutant grew at similar rates when they were each grown in rich culture medium or when inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, the population of the mutant was 14-fold lower than the population of CE3 on bean roots five days after inoculation. CE3003 was significantly less competitive than CE3. A 16,860-fold excess of the mutant in mixed inocula was required to obtain equal nodule occupancy by ....... MenosThe use of Rhizobium inoculants for legumes is restricted by the poor nodulation competitiveness of many inoculant strains. In soil with high indiginous populations of bacteria that can nodulate a given host, the inoculant strains may be found in few or none of the nodules.This presents a problem when the indigenous strains fix little or no nitrogen and therefore do not provide a benefit to host. A strain of Rhizobium is a successful competitor when its representation in the nodules is greater than its representation in the inoculum. To determine whetherthe surface plays a role in nodulation competitiveness of Rhizobium leguminosarum bv.phaseoli (Rlp), I performed an extensive search and analysis of transposon-insertion mutants with altered cell surfaces derived from two nonisogenic parental strains of Rlp, KIM5s and CE3. I isolated and characterized one mutant of CE3, designated CE3003, with a domed colony morphology and a highly hydrophobic cell surface. On bean plants CE3003 was as efficient as CE3 in nodulation and acetylene reduction. The parent and the mutant grew at similar rates when they were each grown in rich culture medium or when inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, the population of the mutant was 14-fold lower than the population of CE3 on bean roots five days after inoculation. CE3003 was significantly less competitive than CE3. A 16,860-fold excess of the mutant in mixed inocula was required to... Mostrar Tudo |
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Palavras-Chave: |
Cell; FBN; Fixação; Fixacao biologica de nitrogenio; NBF; Nitrogen fixing bacteria; phaseoli; Rhizobium leguminosarum bv. |
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Thesagro: |
Bactéria; Célula; Feijão; Inoculante; Legume; Mutação; Nitrogênio; Nodulação; Phaseolus Vulgaris; Rhizobium; Rhizobium Leguminosarum; Simbiose. |
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Thesaurus Nal: |
mutation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02618nam a2200397 a 4500
001 1622453
005 1996-07-18
008 1993 bl uuuu m 00u1 u #d
100 1 $aARAUJO, R. S.
245 $aMutational analysis of the cell surface and nodulation competitiveness of Rhizobium leguminosarum biovar phaseoli.
260 $aMadison: University of Wisconsin$c1993
300 $a118p.
490 $aTese de Doutorado.
500 $aTese de Doutorado. Orientada por: Jo Handelsman.
520 $aThe use of Rhizobium inoculants for legumes is restricted by the poor nodulation competitiveness of many inoculant strains. In soil with high indiginous populations of bacteria that can nodulate a given host, the inoculant strains may be found in few or none of the nodules.This presents a problem when the indigenous strains fix little or no nitrogen and therefore do not provide a benefit to host. A strain of Rhizobium is a successful competitor when its representation in the nodules is greater than its representation in the inoculum. To determine whetherthe surface plays a role in nodulation competitiveness of Rhizobium leguminosarum bv.phaseoli (Rlp), I performed an extensive search and analysis of transposon-insertion mutants with altered cell surfaces derived from two nonisogenic parental strains of Rlp, KIM5s and CE3. I isolated and characterized one mutant of CE3, designated CE3003, with a domed colony morphology and a highly hydrophobic cell surface. On bean plants CE3003 was as efficient as CE3 in nodulation and acetylene reduction. The parent and the mutant grew at similar rates when they were each grown in rich culture medium or when inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, the population of the mutant was 14-fold lower than the population of CE3 on bean roots five days after inoculation. CE3003 was significantly less competitive than CE3. A 16,860-fold excess of the mutant in mixed inocula was required to obtain equal nodule occupancy by .......
650 $amutation
650 $aBactéria
650 $aCélula
650 $aFeijão
650 $aInoculante
650 $aLegume
650 $aMutação
650 $aNitrogênio
650 $aNodulação
650 $aPhaseolus Vulgaris
650 $aRhizobium
650 $aRhizobium Leguminosarum
650 $aSimbiose
653 $aCell
653 $aFBN
653 $aFixação
653 $aFixacao biologica de nitrogenio
653 $aNBF
653 $aNitrogen fixing bacteria
653 $aphaseoli
653 $aRhizobium leguminosarum bv
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 564 | |
| 20. |  | SKOT, L.; ARAUJO, R. S. Introdução de genes em Rhizobium. In: HUNGRIA, M.; ARAUJO, R. S. (Ed.). Manual de métodos empregados em estudos de microbiologia agrícola. Brasília, DF: EMBRAPA-SPI, 1994. p. 201-226. (EMBRAPA-CNPAF. Documentos, 46).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 564 | |
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