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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
05/09/1994 |
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Data da última atualização: |
05/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAUJO, R. S.; ROBLETO, E. A.; HANDELSMAN, J. |
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Afiliação: |
RICARDO SILVA ARAUJO, CNPAF; EDUARDO A. ROBLETO; JO HANDELSMAN. |
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Título: |
A hydrophobic mutant of Rhizobium etli altered in nodulation competitiveness and growth in the rhizosphere. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, v. 60, n. 5, p. 1430-1436, May 1994. |
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DOI: |
https://doi.org/10.1128/aem.60.5.1430-1436.1994 |
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Idioma: |
Inglês |
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Conteúdo: |
We isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surface hydrophobicity, nodulation competitiveness, and competitive growth in the rhizosphere in mutant CE3003. MenosWe isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surfa... Mostrar Tudo |
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Palavras-Chave: |
Hydrophobic; Mutant; PTCA. |
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Thesagro: |
Bactéria; Mutante; Nodulação; Rhizobium. |
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Thesaurus Nal: |
nodulation; Rhizobium etli; rhizosphere. |
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Categoria do assunto: |
B Sociologia Rural |
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Marc: |
LEADER 02428naa a2200277 a 4500
001 1196158
005 2021-10-05
008 1994 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1128/aem.60.5.1430-1436.1994$2DOI
100 1 $aARAUJO, R. S.
245 $aA hydrophobic mutant of Rhizobium etli altered in nodulation competitiveness and growth in the rhizosphere.$h[electronic resource]
260 $c1994
520 $aWe isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surface hydrophobicity, nodulation competitiveness, and competitive growth in the rhizosphere in mutant CE3003.
650 $anodulation
650 $aRhizobium etli
650 $arhizosphere
650 $aBactéria
650 $aMutante
650 $aNodulação
650 $aRhizobium
653 $aHydrophobic
653 $aMutant
653 $aPTCA
700 1 $aROBLETO, E. A.
700 1 $aHANDELSMAN, J.
773 $tApplied and Environmental Microbiology$gv. 60, n. 5, p. 1430-1436, May 1994.
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Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 564 | |
| 63. |  | ARAUJO, R. R.; MILORI, D. M. B. P. Otimização da porcentagem de ligante para análise de metais por DP-LIBS das amostras de solo e sedimento do pantanal mato-grossense. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 28-32| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 66. | | SILVA, S. E. L. da; ARAÚJO, R. da C. O uso das espécies cultivadas. In: SHIFT PROJEKT ENV 23 (Manaus-AM). Recuperação de áreas degradadas e abandonadas, através de sistemas de policultivo. Manaus: EMBRAPA-CPAA: Universidade de Hamburgo, 1996. p. 1-6.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 70. | | LIMA, H. E. de; ALBUQUERQUE, T. C. S. de; ARAÚJO, R. F. de; ARAÚJO, R. S.; QUEIROZ, E. de S.; GUIMARÃES, P. V. P. Recuperação de pomar de cupuaçuzeiro com histórico de alta infestação da doença vassoura-de-bruxa em Roraima. In: CONGRESSO LATINO-AMERICANO DE AGROECOLOGIA, 6.; CONGRESSO BRASILEIRO DE AGROECOLOGIA, 10.; SEMINÁRIO DE AGROECOLOGIA DO DISTRITO FEDERAL E ENTORNO, 5., 2017, Brasília, DF. Agroecologia na transformação dos sistemas agroalimentares na América Latina: memórias, saberes e caminhos para o bem viver: anais. Brasília, DF: Associação Brasileira de Agroecologia, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
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| 71. | | LIMA, H. E. de; ALBUQUERQUE, T. C. S. de; SANTOS, A. G. dos; ARAÚJO, R. F. de; QUEIROZ, E. S.; ARAÚJO, R. S. Técnicas de manejo para recuperação de pomar de cupuaçuzeiro com histórico de alta infestação da doença vassoura-de-bruxa. In: RODRIGUES, T. de A.; LEANDRO NETO, J.; GALVÃO, D. O. (Org.). Meio Ambiente, Sustentabilidade e Agroecologia. Ponta Grossa: Atena Editora, 2019.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Roraima. |
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| 75. | | ARAUJO, R. S.; HENSON, R. A.; MAYA-FLORES, J.; DAZZO, F. B. Avaliação de estirpes de Rhizobium phaseoli no campo. In: REUNIÃO NACIONAL DE PESQUISA DE FEIJÂO, 2., 1987, Goiânia. Resumos. Brasília, DF: EMBRAPA-CNPAF, 1987. Resumo 156. (EMBRAPA-CNPAF. Documentos, 20).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 564 | |
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