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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
24/11/2016 |
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Data da última atualização: |
23/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; ARAUJO, J. L. S. de; BALDANI, J. I. |
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Afiliação: |
PAULA RENATA ALVES DA SILVA, UFRRJ; MARCIA SOARES VIDAL, CNPAB; CLEITON DE PAULA SOARES, BOLSISTA DA EMBRAPA AGROBIOLOGIA; VALERIA POLESE, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoek, v. 109, n. 111, p. 1493-1502, 2016. |
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DOI: |
10.1007/s10482-016-0751-0 |
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Idioma: |
Inglês |
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Notas: |
Epub, 17 Aug., 2016. |
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Conteúdo: |
Among the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice. MenosAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes
carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in
glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon
sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness
by evalua... Mostrar Tudo |
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Palavras-Chave: |
Carbon metabolism; Diazotrophic bacteria; Normalizing genes; Real time PCR; Relative expression. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02573naa a2200265 a 4500
001 2057134
005 2018-10-23
008 2016 bl --- 0-- u #d
024 7 $a10.1007/s10482-016-0751-0$2DOI
100 1 $aSILVA, P. R. A. da
245 $aSelection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice
260 $c2016
500 $aEpub, 17 Aug., 2016.
520 $aAmong the members of the genus Burkholderia, Burkholderia tropica has the ability to fix nitrogen and promote sugarcane plant growth as well as act as a biological control agent. There is little information about how this bacterium metabolizes carbohydrates as well as those carbon sources found in the sugarcane juice that accumulates in stems during plant growth. Reverse transcription quantitative PCR (RT-qPCR) can be used to evaluate changes in gene expression during bacterial growth on different carbon sources. Here we tested the expression of six reference genes, lpxC, gyrB, recA, rpoA, rpoB, and rpoD, when cells were grown with glucose, fructose, sucrose, mannitol, aconitic acid, and sugarcane juice as carbon sources. The lpxC, gyrB, and recA were selected as the most stable reference genes based on geNorm and NormFinder software analyses. Validation of these three reference genes during strain Ppe8 growth on the same carbon sources showed that genes involved in glycogen biosynthesis (glgA, glgB, glgC) and trehalose biosynthesis (treY and treZ) were highly expressed when Ppe8 was grown in aconitic acid relative to other carbon sources, while otsA expression (trehalose biosynthesis) was reduced with all carbon sources. In addition, the expression level of the ORF_6066 (gluconolactonase) gene was reduced on sugarcane juice. The results confirmed the stability of the three selected reference genes (lpxC, gyrB, and recA) during the RT-qPCR and also their robustness by evaluating the relative expression of genes involved in glycogen and trehalose biosynthesis when strain Ppe8 was grown on different carbon sources and sugarcane juice.
653 $aCarbon metabolism
653 $aDiazotrophic bacteria
653 $aNormalizing genes
653 $aReal time PCR
653 $aRelative expression
700 1 $aVIDAL, M. S.
700 1 $aSOARES, C. de P.
700 1 $aPOLESE, V.
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
773 $tAntonie van Leeuwenhoek$gv. 109, n. 111, p. 1493-1502, 2016.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 165 | |
| 121. |  | BALDANI, J. I.; CRUZ, L. M.; VIDAL, M. S.; ARAUJO, J. L. S. de; SCHWAB, S.; TEIXEIRA, K. R. dos S.; SOUZA, E. M.; PEDROSA, F. O.; FARINELLI. Draft genome sequente of six endophytic nittrogen-fixing bacteria with potential biofertilizer application in non-leguminous plants. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. Slll. 05| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 122. | | CORREIA, M. E. F.; PASSOS, S. R.; SOARES, L. H. de B.; ARAÚJO, J. L. S. de; MARTINS, C. M.; XAVIER, G. R.; RUMJANEK, N. G. Ecologia da interação entre fauna do solo e microrganismos no processo de decomposição de resíduos vegetais. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32.; 2 a 7 da agosto de 2009, Fortaleza. O solo e a produção de bionergia: perspectivas e desafios: trabalhos científicos.... Fortaleza: Sociedade Brasileira de Ciência do Solo, 2009. 21p| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 123. | | SOUSA, A. M. B. de; ARAUJO, J. L. S. de; LIMA, M. A. de; FREITAS, A. C. R. de; URQUIAGA, S.; ALVES, B. J. R. Efeito do nitrogênio na população de metanogênicas e emissões de metano, em diferentes cultivares de arroz irrigado. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., FERTBIO 2016. Goiânia. Rumo aos novos desafios: anais. Goiânia: UFG, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Cocais. |
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| 124. | | SOUSA, A. M. B. de; ARAUJO, J. L. S. de; LIMA, M. A. de; FREITAS, A. C. R. de; URQUIAGA, S. S.; ALVES, B. J. R. Efeito do nitrogênio na população de metanogênicas e emissões de metano, em diferentes cultivares de arroz irrigado. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: anais... Goiânia: Sociedade Brasileira de Ciência do Solo, 2016. p. 1045-1046.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 126. | | BOA SORTE, P. M. F.; ARAUJO, J. L. S. de; MELO, L. V. de; GALISA, P. de S.; LEAL, L.; BALDANI, J. I.; BALDANI, V. L. D. Develpment of a real-time PCR assay for the detection and quantification on Gluconacetobacter diazotrophicus in sugarcane grown under field conditions. African Journal of Microbiology Research, v. 8, n. 31, p. 2937-2946, july, 2014| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
|  |
| 127. | | SILVA, P. R. A.; VIDAL, M. S.; POLESE, V.; TADRA-SFEIR, M. Z.; ARAUJO, J. L. S. de; SOUZA, E. M.; BALDANI, J. I. Differential gene expression of diazotrophic burkholderia tropica PPE5 strain cultivated in sugarcane apoplast fluid In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 128. | | ALVES, G. C.; GALISA, P. S.; MACEDO, A. V. de M.; SANTOS, C. L. R. dos; VIDAL, M. S.; ARAUJO, J. L. S. de. Differentially expressed genes in two maize genotypes inoculanted with herbaspirillum seropedicae In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SlV. 07| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 129. | | VIDEIRA, S. S.; GALVAO, P. G.; MORAIS, R. F. de; BALDANI, J. I.; ARAÚJO, J. L. S. de; URQUIAGA, S.; BALDANI, V. L. D. Ocorrência de bactérias diazotróficas associadas a dois cultivares de capim-elefante. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 32.; 2 a 7 da agosto de 2009, Fortaleza. O solo e a produção de bionergia: perspectivas e desafios: trabalhos científicos.... Fortaleza: Sociedade Brasileira de Ciência do Solo, 2009.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 130. | | OSEI, O.; ARAUJO, J. L. S. de; ZILLI, J. E.; BODDEY, R. M.; AHIABOR, B. D. K.; ABAIDOO, R. C.; ROUWS, L. F. M. PCR assay for direct specific detection of Bradyrhizobium elite strain BR 3262 in root nodule extracts of soil-grown cowpea. Plant and Soil, v. 417, n. 1–2, p. 535–548, 2017| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 135. | | SILVA, P. R. A. da; VIDAL, M. S.; TADRA-SFEIR, M.Z.; ARAUJO, J. L. S. de; SOUZA, E. M. de; BALDANI, J. I. In vitro study suggests that stem apoplastic liquid of sugarcance modulates the chemotaxis and defense mechanism of the diazotrophic Burkholderia tropica strain PPe8 In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 15.; SYMPOSIUM OF THE EUROPEAN NITROGEN FIXATION CONFERENCE, 12., 2016, Hungary. Book of abstracts. Hungary: Biological Research Center, 2016. p. 47| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 136. | | LEITE, J.; PASSOS, S. R.; ARAUJO, J. L. S. de; RUMJANEK, N. G.; XAVIER, G. R.; ZILLI, J. E.; RADL, V. What we know about the novel legume-nodulating Microvirga vignae? In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. 308 p. Editado por Mariangle Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho. RELAR 2016. p. 169| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 137. | | ARAUJO, J. L. S. de; LEITE, J.; ROUWS, L. F. M.; PASSOS, S. R.; XAVIER, G. R.; RUMJANEK, N. G.; ZILLI, J. E. WITHDRAWN: draft genome sequence of Bradyrhizobium pachyrhizi strain BR 3262, an effective microsymbiont recommended for cowpea inoculation in Brazil. Brazilian Journal of Microbiology, v. 47, n. 4, p. 783-784, oct., 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 138. | | GARCIA, O. S. R.; ARAUJO, J. L. S. de; GASPARINO, E.; RODRIGUES, M. T.; KHATLAB, A. de S.; PAULINO, P. V. R.; SILVA, J. C. da; AZEVEDO, P. C. N. de; SOARES, M. A. M. Association of CAST-gene polymorphism with mRNA levels and meat tenderness in goats. Animal Production Science, v., n. 11, p. 1393-1401, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 139. | | SILVA, C. F. da; ARAUJO, J. L. S. de; SILVA, E. M. R. da; PEREIRA, M. G.; SCHIAVO, J. A.; FREITAS, M. S. M. de; SAGGIN JUNIOR, O. J.; MARTINS, M. A. Comunidade de fungos micorrízicos arbusculares: diversidade, composição e glomalina em área revegetada com sesbânia. Revista Brasileira de Ciência do Solo, v. 38, n. 2, p. 423-431, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 140. | | MICHEL, D. C.; PASSOS, S. R.; ARAUJO, J. L. S. de; BARAÚNA, A. C.; SILVA, K. da; LEME, M. M. P.; MELO, I. S. de; DE MEYER, S. E.; O’HARAM G.; ZILLI, J. E. Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil. Archives of Microbiology, v. 199, n. 5, p. 657-664, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia; Embrapa Roraima. |
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| Registros recuperados : 165 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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