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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
05/03/2024 |
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Data da última atualização: |
05/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, H. A. P. da; CAETANO, V. S.; PESSÔA, D. D. V.; PACHECO, R. S.; MENESES, C. H. S. G.; ARAUJO, J. L. S. de. |
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Afiliação: |
HELDER ANDERSON PINTO DA SILVA, UFRJ; VANESSA SANTANA CAETANO, UFRJ; DANIELLA DUARTE VILLARINHO PESSÔA, UFRJ; RAFAEL SANCHES PACHECO, UFRRJ; CARLOS HENRIQUE S. G. MENESES, UEPB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
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Título: |
Unraveling the drought-responsive transcriptomes in nodules of two common bean genotypes during biological nitrogen fixation. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 152024, 1345379, 2024 |
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ISSN: |
1664462X |
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DOI: |
10.3389/fpls.2024.1345379 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to drought stress during BNF. BAT 477 genotype exhibited more pronounced response to drought, characterized by a high number of DEGs. The strong down-regulation of DEGs leads to transcriptional disturbances in several pathways related to stress acclimation such as hormone and antioxidant metabolism. Additionally, we identified several genes that are known to play key roles in enhancing drought tolerance, such as HSPs and crucial TFs. Our results provide new insights into the transcriptional responses in root-nodules, an underexplored tissue of plants mainly under drought conditions. This research paves the way for potential improvements in plant-bacteria interactions, contributing to common bean adaptations in the face of challenging environmental conditions MenosCommon bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to ... Mostrar Tudo |
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Palavras-Chave: |
Candidate genes; Genetic transcript profile; RT-qPCR; Symbiotic nitrogen assimilation; Water deficit. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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008 2024 bl uuuu u00u1 u #d
022 $a1664462X
024 7 $a10.3389/fpls.2024.1345379$2DOI
100 1 $aSILVA, H. A. P. da
245 $aUnraveling the drought-responsive transcriptomes in nodules of two common bean genotypes during biological nitrogen fixation.$h[electronic resource]
260 $c2024
520 $aCommon bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to drought stress during BNF. BAT 477 genotype exhibited more pronounced response to drought, characterized by a high number of DEGs. The strong down-regulation of DEGs leads to transcriptional disturbances in several pathways related to stress acclimation such as hormone and antioxidant metabolism. Additionally, we identified several genes that are known to play key roles in enhancing drought tolerance, such as HSPs and crucial TFs. Our results provide new insights into the transcriptional responses in root-nodules, an underexplored tissue of plants mainly under drought conditions. This research paves the way for potential improvements in plant-bacteria interactions, contributing to common bean adaptations in the face of challenging environmental conditions
653 $aCandidate genes
653 $aGenetic transcript profile
653 $aRT-qPCR
653 $aSymbiotic nitrogen assimilation
653 $aWater deficit
700 1 $aCAETANO, V. S.
700 1 $aPESSÔA, D. D. V.
700 1 $aPACHECO, R. S.
700 1 $aMENESES, C. H. S. G.
700 1 $aARAUJO, J. L. S. de
773 $tFrontiers in Plant Science$gv. 152024, 1345379, 2024
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 163 | |
| 121. |  | GALISA, P. S.; SILVA, H. A. P. da; MACEDO, A. V. de M.; REIS, V. M.; VIDAL, M. S.; BALDANI, J. I.; ARAUJO, J. L. S. de. Identification and validation of reference genes to study the gene expression in Gluconacetobacter diazotrophicus grown in different carbon source using RT-qPCR. Journal of Microbiological Methods, v. 91, p. 1-7, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 122. | | SILVA, J. R. da; GONÇALO, T. P.; GALISA, de S.; SILVA, H. A P. da; SCORTECCI, K. C.; ARAUJO, J. L. S. de; VIDAL, M. S. Identificação de genes diferencialmente expressos em cana-de-açúcar submetida a estresse hídrico. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 123. | | GOLÇALO, T. P.; SILVA, R. da; SILVA, H. A. P. da; GALISA, P. de S.; VIDAL, M. S.; ARAUJO, J. L. S. de. Identificação de genes do feijoeiro envolvidos na simbiose com o Rhizobium tropici. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 124. | | GONÇALO, T. P.; SILVA, J. R. da; SILVA, H. A.; P. da; GALISA, P. de S.; VIDAL, M. S.; ARAÚJO, J. L. S. de. Isolamento e caracterização de genes do feijoeiro envolvidos na simbiose com Rhizobium tropici. In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009.| Biblioteca(s): Embrapa Agrobiologia. |
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| 126. | | LEITE, J.; PASSOS, S. R.; ARAUJO, J. L. S. de; RUMJANEK, N. G.; XAVIER, G. R.; ZILLI, J. E.; RADL, V. What we know about the novel legume-nodulating Microvirga vignae? In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. 308 p. Editado por Mariangle Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho. RELAR 2016. p. 169| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 127. | | ARAUJO, J. L. S. de; LEITE, J.; ROUWS, L. F. M.; PASSOS, S. R.; XAVIER, G. R.; RUMJANEK, N. G.; ZILLI, J. E. WITHDRAWN: draft genome sequence of Bradyrhizobium pachyrhizi strain BR 3262, an effective microsymbiont recommended for cowpea inoculation in Brazil Brazilian Journal of Microbiology, v. 47, n. 4, p. 783-784, oct., 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 128. | | SILVA, P. R. A. da; VIDAL, M. S.; TADRA-SFEIR, M.Z.; ARAUJO, J. L. S. de; SOUZA, E. M. de; BALDANI, J. I. In vitro study suggests that stem apoplastic liquid of sugarcance modulates the chemotaxis and defense mechanism of the diazotrophic Burkholderia tropica strain PPe8 In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 15.; SYMPOSIUM OF THE EUROPEAN NITROGEN FIXATION CONFERENCE, 12., 2016, Hungary. Book of abstracts. Hungary: Biological Research Center, 2016. p. 47| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 129. | | SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; ARAUJO, J. L. S. de; BALDANI, J. I. Selection and evaluation of reference genes for RT-qPCR expression studies on Burkholderia tropica strain Ppe8, a sugarcane-associated diazotrophic bacterium grown with different carbon sources or sugarcane juice Antonie van Leeuwenhoek, v. 109, n. 111, p. 1493-1502, 2016. Epub, 17 Aug., 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 130. | | POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 131. | | SOARES, C. de P.; RODRIGUES, E. P.; FERREIRA, J. P.; ARAUJO, J. L. S. de; ROUWS, L. F. M.; BALDANI, J. I.; VIDAL, M. S. Tn5 insertion in the tonB gene promoter affects iron-related phenotypes and increases extracellular siderophore levels in Gluconacetobacter diazotrophicus Archives of Microbiology Online, 17 oct. 2014. 11 p. A versão impressa foi publicada no v. 197, p. 223-233, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 132. | | POLESE, V.; SILVA, P. R. A.; PESSOA, D. D. V.; TADRA-SFEIR, M. Z.; SOUZA, E. M.; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. Transcriptional profile of Herbaspirillum rubrisubalbicans in response of sugarcane apoplastic fluid. In: WORLD CONGRESS ON GENETICS APPLIED TO LIVESTOCK PRODUCTION, 11., 2018, Auckland, New Zealand. Proceedings... Massey University, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 133. | | SOARES, C. P.; MENESES, C. H. S. G; RODRIGUES, E. P.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; VIDAL, M. S.; BALDANI, J. I. TonB mutant of Gluconacetobacter Diazotrophicus PAL5 is defective in biofilm formation. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SlV.23| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 134. | | OSEI, O.; ARAUJO, J. L. S. de; ZILLI, J. E.; BODDEY, R. M.; AHIABOR, B. D. K.; ABAIDOO, R. C.; ROUWS, L. F. M. Strain specif PCR assay for determination of nodule occupancy grain legumes by the elite-strain BR 3262 in Ghana. In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. 308 p. Editado por Mariangle Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho. RELAR 2016. p. 300| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 138. | | GARCIA, O. S. R.; ARAUJO, J. L. S. de; GASPARINO, E.; RODRIGUES, M. T.; KHATLAB, A. de S.; PAULINO, P. V. R.; SILVA, J. C. da; AZEVEDO, P. C. N. de; SOARES, M. A. M. Association of CAST-gene polymorphism with mRNA levels and meat tenderness in goats. Animal Production Science, v., n. 11, p. 1393-1401, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 139. | | SERRATO, R. V.; MENESES, C. H. G de; SASSAKI, G. L; SANTANA FILHO, A. P.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; CHAVES, M. S.; IACOMINI, M.; BALDANI, J. I. Chemical and structural analysis on the exopolysaccharide produced by the nitrogen-fixing bacterium Gluconacetobacter diazotrophicus Pal5 In: ANNUAL MEETING OF BRAZILIAN BIOCHEMISTRY AND MOLECULAR BIOLOGY SOCIETY, 40., 2011, Foz do Iguaçu. Abstracts. São Paulo: SBBq, 2011| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 140. | | SILVA, C. F. da; ARAUJO, J. L. S. de; SILVA, E. M. R. da; PEREIRA, M. G.; SAGGIN JUNIOR, O. J.; FREITAS, M. S. M. de; MARTINS, M. A. Fungos micorrízicos arbusculares e proteína do solo relacionada à glomalina em àrea degradada por extração de argila e revegetada com Eucalipto e Acácia. Ciência Florestal, Santa Maria, v. 22, n. 4, p. 757-769, out. dez., 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 163 | |
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