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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
09/11/2021 |
Data da última atualização: |
09/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOARES, I. C.; PACHECO, R. S.; SILVA, C. G. N. da; SANTOS, R. S.; BALDANI, J. I.; URQUIAGA, S.; VIDAL, M. S.; ARAUJO, J. L. S. de. |
Afiliação: |
ISIS CAPELLA SOARES, UFRRJ; RAFAEL SANCHES PACHECO, UFRRJ; CLEUDISON GABRIEL NASCIMENTO DA SILVA, UFLA; RAFAEL SALAZAR SANTOS, UFRRJ; JOSE IVO BALDANI, CNPAB; SEGUNDO SACRAMENTO U CABALLERO, CNPAB; MARCIA SOARES VIDAL, CNPAB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
Título: |
Real-time PCR method to quantify Sp245 strain of Azospirillum baldaniorum on Brachiaria grasses under field conditions. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Plant and Soil, 06 September 2021 |
ISSN: |
0032-079X |
DOI: |
https://doi.org/10.1007/s11104-021-05137-y |
Idioma: |
Inglês |
Conteúdo: |
Bacterial quantification by qPCR is considered the gold standard for microbial molecular diagnosis. However, a fundamental pre-requisite in this methodology is the designing of specific primers for the bacterium of interest. With the increase in bacterial genome sequencing data in the recent years, it has become possible to design specific primers that can be used to quantify different strains of the same bacterial species. Methods: To develop a real-time PCR (qPCR) protocol for the specific quantification of Azospirillum baldaniorum Sp245 strain (old Azospirillum brasilense), the Sp245 genome sequence was fragmented into small contigs with 500 base pairs each, and analyzed for similarity against the NCBI non-redundant database. A. baldaniorum-specific contigs were used to design the primers. The best pair of primers was used to quantify these bacteria after inoculation in different cultivars of Brachiaria, grown under field conditions. Results: Our results showed that the primer pair Sp245p10 was highly specific for the Sp245 strain in the Brachiaria root and shoot field under different conditions. The qPCR assay using these primers showed differences among cultivars in the number of bacteria detected in plants after inoculation. Additionally, the number of bacteria observed in the roots was higher than that in the shoots. Conclusion: The qPCR methodology using a Sp245 strain-specific primer may be used to monitor A. baldaniorum inoculated into other plants and may find potential application in field experiments. MenosBacterial quantification by qPCR is considered the gold standard for microbial molecular diagnosis. However, a fundamental pre-requisite in this methodology is the designing of specific primers for the bacterium of interest. With the increase in bacterial genome sequencing data in the recent years, it has become possible to design specific primers that can be used to quantify different strains of the same bacterial species. Methods: To develop a real-time PCR (qPCR) protocol for the specific quantification of Azospirillum baldaniorum Sp245 strain (old Azospirillum brasilense), the Sp245 genome sequence was fragmented into small contigs with 500 base pairs each, and analyzed for similarity against the NCBI non-redundant database. A. baldaniorum-specific contigs were used to design the primers. The best pair of primers was used to quantify these bacteria after inoculation in different cultivars of Brachiaria, grown under field conditions. Results: Our results showed that the primer pair Sp245p10 was highly specific for the Sp245 strain in the Brachiaria root and shoot field under different conditions. The qPCR assay using these primers showed differences among cultivars in the number of bacteria detected in plants after inoculation. Additionally, the number of bacteria observed in the roots was higher than that in the shoots. Conclusion: The qPCR methodology using a Sp245 strain-specific primer may be used to monitor A. baldaniorum inoculated into other plants and may find pot... Mostrar Tudo |
Palavras-Chave: |
Azospirillum baldanioru; Plant growth promoting bacteria; Quantification; Signal grass. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02397naa a2200277 a 4500 001 2135952 005 2021-11-09 008 2021 bl uuuu u00u1 u #d 022 $a0032-079X 024 7 $ahttps://doi.org/10.1007/s11104-021-05137-y$2DOI 100 1 $aSOARES, I. C. 245 $aReal-time PCR method to quantify Sp245 strain of Azospirillum baldaniorum on Brachiaria grasses under field conditions.$h[electronic resource] 260 $c2021 520 $aBacterial quantification by qPCR is considered the gold standard for microbial molecular diagnosis. However, a fundamental pre-requisite in this methodology is the designing of specific primers for the bacterium of interest. With the increase in bacterial genome sequencing data in the recent years, it has become possible to design specific primers that can be used to quantify different strains of the same bacterial species. Methods: To develop a real-time PCR (qPCR) protocol for the specific quantification of Azospirillum baldaniorum Sp245 strain (old Azospirillum brasilense), the Sp245 genome sequence was fragmented into small contigs with 500 base pairs each, and analyzed for similarity against the NCBI non-redundant database. A. baldaniorum-specific contigs were used to design the primers. The best pair of primers was used to quantify these bacteria after inoculation in different cultivars of Brachiaria, grown under field conditions. Results: Our results showed that the primer pair Sp245p10 was highly specific for the Sp245 strain in the Brachiaria root and shoot field under different conditions. The qPCR assay using these primers showed differences among cultivars in the number of bacteria detected in plants after inoculation. Additionally, the number of bacteria observed in the roots was higher than that in the shoots. Conclusion: The qPCR methodology using a Sp245 strain-specific primer may be used to monitor A. baldaniorum inoculated into other plants and may find potential application in field experiments. 653 $aAzospirillum baldanioru 653 $aPlant growth promoting bacteria 653 $aQuantification 653 $aSignal grass 700 1 $aPACHECO, R. S. 700 1 $aSILVA, C. G. N. da 700 1 $aSANTOS, R. S. 700 1 $aBALDANI, J. I. 700 1 $aURQUIAGA, S. 700 1 $aVIDAL, M. S. 700 1 $aARAUJO, J. L. S. de 773 $tPlant and Soil, 06 September 2021
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Embrapa Agrobiologia (CNPAB) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Territorial. Para informações adicionais entre em contato com cnpm.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Territorial. |
Data corrente: |
08/01/2019 |
Data da última atualização: |
08/01/2019 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
VILELA, G. F.; BENTES, M. P. de M.; OLIVEIRA, Y. M. M. de; MARQUES, D. K. S.; SILVA, J. C. B. (ed.). |
Afiliação: |
GISELE FREITAS VILELA, CNPM; MICHELLINY PINHEIRO DE MATOS BENTES, CPATU; YEDA MARIA MALHEIROS DE OLIVEIRA, CNPF; DEBORA KARLA SILVESTRE MARQUES, CPAP; JULIANA CORREA BORGES SILVA, CPAP. |
Título: |
Vida terrestre: contribuições da Embrapa. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Brasília, DF: Embrapa, 2018. |
Série: |
(Objetivos de Desenvolvimento Sustentável, 15). |
ISBN: |
978-85-7035-795-3 |
Idioma: |
Português |
Conteúdo: |
Este volume apresenta as contribuições da Empresa Brasileira de Pesquisa Agropecuária (Embrapa) ao Objetivo de Desenvolvimento Sustentável (ODS) 15, cujo tema é Vida Terrestre: Proteger, recuperar e promover o uso sustentável dos ecossistemas terrestres, gerir de forma sustentável as florestas, combater a desertificação, deter e reverter a degradação da terra e deter a perda de biodiversidade. |
Palavras-Chave: |
Agricultura de montanha. |
Thesagro: |
Biossegurança; Conservação; Controle biológico; Desenvolvimento sustentável; Desmatamento; Ecossistema; Políticas Públicas; Recuperação do Solo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01279naa a2200301 a 4500 001 2103308 005 2019-01-08 008 2018 bl uuuu u00u1 u #d 020 $a978-85-7035-795-3 100 1 $aVILELA, G. F. 245 $aVida terrestre$bcontribuições da Embrapa.$h[electronic resource] 260 $c2018 490 $a(Objetivos de Desenvolvimento Sustentável, 15). 520 $aEste volume apresenta as contribuições da Empresa Brasileira de Pesquisa Agropecuária (Embrapa) ao Objetivo de Desenvolvimento Sustentável (ODS) 15, cujo tema é Vida Terrestre: Proteger, recuperar e promover o uso sustentável dos ecossistemas terrestres, gerir de forma sustentável as florestas, combater a desertificação, deter e reverter a degradação da terra e deter a perda de biodiversidade. 650 $aBiossegurança 650 $aConservação 650 $aControle biológico 650 $aDesenvolvimento sustentável 650 $aDesmatamento 650 $aEcossistema 650 $aPolíticas Públicas 650 $aRecuperação do Solo 653 $aAgricultura de montanha 700 1 $aBENTES, M. P. de M. 700 1 $aOLIVEIRA, Y. M. M. de 700 1 $aMARQUES, D. K. S. 700 1 $aSILVA, J. C. B. 773 $tBrasília, DF: Embrapa, 2018.
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