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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
02/02/2017 |
Data da última atualização: |
19/10/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DELAMUTA, J. R. M.; RIBEIRO, R. A.; ARAUJO, J. L. S. de; ROUWS, L. F. M.; ZILLI, J. E.; PARMA, M. M.; MELO, I. S. de; HUNGRIA, M. |
Afiliação: |
JAKELINE RENATA MARCON DELAMUTA, CAPES; RENAN AUGUSTO RIBEIRO, CNPq; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB; JERRI EDSON ZILLI, CNPAB; MARCIA MARIA PARMA LEME, CNPMA; ITAMAR SOARES DE MELO, CNPMA; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
Título: |
Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, v. 66, n. 8, p. 3078-3087, 2016. |
DOI: |
http://dx.doi.org/10.1099/ijsem.0.001148 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446T, BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense, and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4 % similarity to all described species of the genus Bradyrhizobium. Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with <96.5 % nucleotide identity with other members of the genus Bradyrhizobium. Average nucleotide identity (ANI) of genome sequences between strain BR 446T and B.huanghuaihaiense was below the threshold for species circumscription (90.7 %). DNA-DNA hybridization resulted in ΔTm values over 6.7 °C with the most closely related species. Similarities among the BR strains and differences from other species were confirmed by rep-PCR analysis. Interestingly, the BR strains were grouped in the analysis of nifH and nodC genes, but showed higher similarity with B. iriomotense and B. manausense than with B.huanghuaihaiense, indicating a different evolutionary history for nitrogen-fixation genes. Morpho-physiological, genotypic and genomic data supported that these BR strains represent a novel species for which the name Bradyrhizobium stylosanthis sp. nov. is suggested. The type strain is BR 446T (=CNPSo 2823T=HAMBI 3668T=H-8T), isolated from Stylosanthes guianensis. MenosAbstract: The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446T, BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense, and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4 % similarity to all described species of the genus Bradyrhizobium. Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with <96.5 % nucleotide identity with other members of the genus Bradyrhizobium. Average nucleotide identity (ANI) of genome sequences between strain BR 446T and B.huanghuaihaiense was below the threshold for species circumscription (90.7 %). DNA-DNA hybridization resulted in ΔTm values over 6.7 °C with the most closely related species. Similarities among the BR strains and differences from other species were confirmed by rep-PCR analysis. Interestingly, the BR strains were grouped in the analysis of nifH and nodC genes, but showed higher similarity with B. iriomotense and B. manau... Mostrar Tudo |
Palavras-Chave: |
Bradyrhizobium stylosanthis. |
Thesagro: |
Fixação simbiótica de nitrogênio; Planta forrageira; Rhizobium. |
Thesaurus Nal: |
Nitrogen-fixing bacteria; Stylosanthes. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02886naa a2200289 a 4500 001 2062548 005 2020-10-19 008 2016 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1099/ijsem.0.001148$2DOI 100 1 $aDELAMUTA, J. R. M. 245 $aBradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.$h[electronic resource] 260 $c2016 520 $aAbstract: The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446T, BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense, and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4 % similarity to all described species of the genus Bradyrhizobium. Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with <96.5 % nucleotide identity with other members of the genus Bradyrhizobium. Average nucleotide identity (ANI) of genome sequences between strain BR 446T and B.huanghuaihaiense was below the threshold for species circumscription (90.7 %). DNA-DNA hybridization resulted in ΔTm values over 6.7 °C with the most closely related species. Similarities among the BR strains and differences from other species were confirmed by rep-PCR analysis. Interestingly, the BR strains were grouped in the analysis of nifH and nodC genes, but showed higher similarity with B. iriomotense and B. manausense than with B.huanghuaihaiense, indicating a different evolutionary history for nitrogen-fixation genes. Morpho-physiological, genotypic and genomic data supported that these BR strains represent a novel species for which the name Bradyrhizobium stylosanthis sp. nov. is suggested. The type strain is BR 446T (=CNPSo 2823T=HAMBI 3668T=H-8T), isolated from Stylosanthes guianensis. 650 $aNitrogen-fixing bacteria 650 $aStylosanthes 650 $aFixação simbiótica de nitrogênio 650 $aPlanta forrageira 650 $aRhizobium 653 $aBradyrhizobium stylosanthis 700 1 $aRIBEIRO, R. A. 700 1 $aARAUJO, J. L. S. de 700 1 $aROUWS, L. F. M. 700 1 $aZILLI, J. E. 700 1 $aPARMA, M. M. 700 1 $aMELO, I. S. de 700 1 $aHUNGRIA, M. 773 $tInternational Journal of Systematic and Evolutionary Microbiology$gv. 66, n. 8, p. 3078-3087, 2016.
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Embrapa Meio Ambiente (CNPMA) |
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Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
29/07/2021 |
Data da última atualização: |
18/08/2021 |
Tipo da produção científica: |
Circular Técnica |
Autoria: |
GODOY, C. V.; UTIAMADA, C. M.; MEYER, M. C.; CAMPOS, H. D.; LOPES, I. de O. N.; TOMEN, A.; SICHOCKI, D.; MOREIRA, E. N.; KONAGESKI, F. T.; BONANI, J. C.; NUNES JUNIOR, J.; BELUFI, L. M. de R.; FANTIN, L. H.; LIMA, L. A. de S.; SILVA, L. H. C. P. da; ARAÚJO JÚNIOR, I. P.; GOUSSAIN JÚNIOR, M.; GARBIATE, M. V.; MÜLLER, M. A.; MARTINS, M. C.; TORMEN, N. R.; KONAGESKI, T. F.; CARLIN, V. J. |
Afiliação: |
CLAUDIA VIEIRA GODOY, CNPSO; ENGENHEIRO-AGRÔNOMO, TAGRO TECNOLOGIA AGROPECUÁRIA LTDA., LONDRINA, PR; MAURICIO CONRADO MEYER, CNPSO; ENGENHEIRO-AGRÔNOMO, DOUTOR, UNIVERSIDADE DE RIO VERDE, RIO VERDE, GO; IVANI DE OLIVEIRA NEGRAO LOPES, CNPSO; ENGENHEIRA-AGRÔNOMA, MESTRE, PROTEPLAN PESQUISA E ASSESSORIA AGRÍCOLA LTDA., SORRISO, MT; ENGENHEIRO-AGRÔNOMO, MESTRE, META CONSULTORIA AGRÍCOLA, CANARANA, MT; ENGENHEIRO-AGRÔNOMO, DOUTOR, FITOLAB PESQUISA E DESENVOLVIMENTO AGRÍCOLA, SORRISO, MT; ENGENHEIRA-AGRÔNOMA, RURAL TÉCNICA EXPERIMENTOS, QUERÊNCIA, MT; ENGENHEIRO-AGRÔNOMO, COAMO, CAMPO MOURÃO, PR; ENGENHEIRO-AGRÔNOMO, DOUTOR, CENTRO TECNOLÓGICO PARA PESQUISAS AGROPECUÁRIAS - CTPA, GOIÂNIA, GO; ENGENHEIRA-AGRÔNOMA, MESTRE, FUNDAÇÃO DE PESQUISA E DESENVOLVIMENTO TECNOLÓGICO RIO VERDE, LUCAS DO RIO VERDE, MT; ENGENHEIRO-AGRÔNOMO, DOUTOR, FUNDAÇÃO CHAPADÃO, CHAPADÃO DO SUL, MS; ENGENHEIRO-AGRÔNOMO, META CONSULTORIA AGRÍCOLA, CANARANA, MT; ENGENHEIRO-AGRÔNOMO, MESTRE, AGRO CARREGAL PESQUISA E PROTEÇÃO DE PLANTAS EIRELI, RIO VERDE, GO; ENGENHEIRO-AGRÔNOMO, PROTEPLAN PESQUISA E ASSESSORIA AGRÍCOLA LTDA., SORRISO, MT; ENGENHEIRO-AGRÔNOMO, DOUTOR, ASSIST CONSULTORIA E EXPERIMENTAÇÃO AGRONÔMICA LTDA., CAMPO VERDE, MT; ENGENHEIRO-AGRÔNOMO, COAMO, CAMPO MOURÃO, PR; ENGENHEIRA-AGRÔNOMA, DOUTORA, FUNDAÇÃO MATO GROSSO, RONDONÓPOLIS, MT; ENGENHEIRA-AGRÔNOMA, DOUTORA, CÍRCULO VERDE ASSESSORIA AGRONÔMICA E PESQUISA, LUÍS EDUARDO MAGALHÃES, BA; ENGENHEIRO-AGRÔNOMO, DOUTOR, INSTITUTO PHYTUS, PLANALTINA, DF; ENGENHEIRO-AGRÔNOMO, RURAL TÉCNICA EXPERIMENTOS AGRONÔMICOS LTDA., QUERÊNCIA, MT; (IN MEMORIAM) ENGENHEIRO-AGRÔNOMO, AGRODINÂMICA, TANGARÁ DA SERRA, MT. |
Título: |
Eficiência de fungicidas para o controle da mancha-alvo, Corynespora cassiicola, na cultura da soja, na safra 2020/2021: resultados sumarizados dos ensaios cooperativos. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Londrina: Embrapa Soja, 2021. |
Páginas: |
13 p. |
Série: |
(Embrapa Soja. Circular técnica, 172). |
Idioma: |
Português |
Thesagro: |
Corynespora Cassiicola; Doença de Planta; Fungicida; Mancha Alvo; Safra; Soja. |
Thesaurus NAL: |
Fungicides; Plant diseases and disorders; Soybeans. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225309/1/Circ-Tec-172-OL.pdf
|
Marc: |
LEADER 01460nam a2200493 a 4500 001 2133167 005 2021-08-18 008 2021 bl uuuu u0uu1 u #d 100 1 $aGODOY, C. V. 245 $aEficiência de fungicidas para o controle da mancha-alvo, Corynespora cassiicola, na cultura da soja, na safra 2020/2021$bresultados sumarizados dos ensaios cooperativos.$h[electronic resource] 260 $aLondrina: Embrapa Soja$c2021 300 $a13 p. 490 $a(Embrapa Soja. Circular técnica, 172). 650 $aFungicides 650 $aPlant diseases and disorders 650 $aSoybeans 650 $aCorynespora Cassiicola 650 $aDoença de Planta 650 $aFungicida 650 $aMancha Alvo 650 $aSafra 650 $aSoja 700 1 $aUTIAMADA, C. M. 700 1 $aMEYER, M. C. 700 1 $aCAMPOS, H. D. 700 1 $aLOPES, I. de O. N. 700 1 $aTOMEN, A. 700 1 $aSICHOCKI, D. 700 1 $aMOREIRA, E. N. 700 1 $aKONAGESKI, F. T. 700 1 $aBONANI, J. C. 700 1 $aNUNES JUNIOR, J. 700 1 $aBELUFI, L. M. de R. 700 1 $aFANTIN, L. H. 700 1 $aLIMA, L. A. de S. 700 1 $aSILVA, L. H. C. P. da 700 1 $aARAÚJO JÚNIOR, I. P. 700 1 $aGOUSSAIN JÚNIOR, M. 700 1 $aGARBIATE, M. V. 700 1 $aMÜLLER, M. A. 700 1 $aMARTINS, M. C. 700 1 $aTORMEN, N. R. 700 1 $aKONAGESKI, T. F. 700 1 $aCARLIN, V. J.
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