Data/hora: 01/06/2024 06:30:30

Biblioteca(s): 	Embrapa Meio Ambiente.	
Data corrente: 	23/10/2017	
Data da última atualização: 	02/04/2018	
Autoria: 	MICHEL, D. C.; PASSOS, S. R.; ARAUJO, J. L. S. de; BARAÚNA, A. C.; SILVA, K. da; LEME, M. M. P.; MELO, I. S. de; DE MEYER, S. E.; O'HARAM, G.; ZILLI, J. E.	
Afiliação: 	DANIELE C. MICHEL, UFRRJ; SAMUEL R. PASSOS, BOLSISTA DA EMBRAPA AGROBIOLOGIA; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; ALEXANDRE C. BARAÚNA, UFRRH; KRISLE DA SILVA, CPAF-Roraima; MARCIA MARIA PARMA LEME, CNPMA; ITAMAR SOARES DE MELO, CNPMA; SOFIE E. DE MEYER, CENTRE FOR RHIZOBIUM STUDIES, MURDOCH UNIVERSITY; GRAHAM O'HARA, CENTRE FOR RHIZOBIUM STUDIES, MURDOCH UNIVERSITY; JERRI EDSON ZILLI, CNPAB.	
Título: 	Bradyrhizobium centrolobii and Bradyrhizobium macuxiense sp. nov. isolated from Centrolobium paraense grown in soil of Amazonia, Brazil	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Archives of Microbiology, v. 199, n. 5, p. 675-664, 2017.	
DOI: 	10.1007/s00203-017-1340-y	
Idioma: 	Inglês	
Conteúdo: 	Thirteen Gram-negative, aerobic, motile with polar flagella, rod-shaped bacteria were isolated from root nodules of Centrolobium paraense Tul. grown in soils from the Amazon region of Brazil. Growth of strains was observed at temperature range 20-36?°C (optimal 28?°C), pH ranges 5-11 (optimal 6.0-7.0), and 0.1-0.5%NaCl (optimal 0.1-0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were 12 species with more than 99% of similarity. Multi-locus sequence analysis (MLSA) with dnaK, glnII, recA, and rpoB confirmed B. neotropicale BR 10247T as the closest type strain for the group I and B. elkanii USDA 76T and B. pachyrhizi PAC 48T for group II. Average Nucleotide Identity (ANI) differentiated group I from the B. neotropicale BR 10247T (79.6%) and group II from B. elkanii USDA 76T and B. pachyrhizi PAC 48T (88.1% and 87.9%, respectively). Fatty acid profiles [majority C16:0 and Summed feature 8 (18:1?6c/18:1?7c) for both groups], DNA G?+?C content, and carbon compound utilization supported the placement of the novel strains in the genus Bradyrhizobium. Gene nodC and nifH of the new strains have in general low similarity with other Bradyrhizobium species. Both groups nodulated plants from the tribes Crotalarieae, Dalbergiae, Genisteae, and Phaseoleae. Based on the presented data, two novel species which the names Bradyrhizobium centrolobii and Bradyrhizobium macuxiense are proposed, with BR 10245T (=HAMBI 3597T) and BR 10303T (=HAMBI 3602T) as the respective-type strains.	
Palavras-Chave: 	Average nucleotide identity; Fixação simbiótica do nitrogênio; Nitrogen fixing bacteria; Symbiontes.	
Thesagro: 	Leguminosa; Nodulação.	
Thesaurus Nal: 	Chemotaxonomy; Nodulation; Phylogeny.	
Categoria do assunto: 	V Taxonomia de Organismos	
Registro original: 	Embrapa Meio Ambiente (CNPMA)	
Exemplares: 	CNPMA - (15770-1) - UPC - SP - DD -  -  -  - DIS