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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
16/03/2010 |
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Data da última atualização: |
16/03/2010 |
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Tipo da produção científica: |
Folder/Folheto/Cartilha |
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Autoria: |
SOARES, C. P.; VIDAL, M. S.; ARAÚJO, J. L. S. |
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Afiliação: |
Cleiton de Paula Soares, Embrapa Agrobiologia; Marcia Soares vidal, Embrapa Agrobiologia; Jean Luiz simões de araújo, Embrapa Agrobiologia. |
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Título: |
Resposta de eEscherichia Coli Expressando a gene VuHPS 17.7 frente a estresse oxidativo |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009. Seropédica: Embrapa Agrobiologia, 2009 |
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Volume: |
01 |
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Páginas: |
01 |
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Idioma: |
Português |
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Palavras-Chave: |
Estresse oxidativo; Gene VuHSP 17.7. |
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Thesagro: |
Escherichia coli. |
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Categoria do assunto: |
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Marc: |
LEADER 00591nam a2200181 a 4500
001 1661410
005 2010-03-16
008 2009 bl uuuu u0uu1 u #d
100 1 $aSOARES, C. P.
245 $aResposta de eEscherichia Coli Expressando a gene VuHPS 17.7 frente a estresse oxidativo
260 $aIn: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009. Seropédica: Embrapa Agrobiologia$c2009
300 $a01 01
490 $v01
650 $aEscherichia coli
653 $aEstresse oxidativo
653 $aGene VuHSP 17.7
700 1 $aVIDAL, M. S.
700 1 $aARAÚJO, J. L. S.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
LEVY, S. M.; FALLEIROS, A. M. F.; MOSCARDI, F.; GREGÓRIO, E. A. |
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Título: |
Is the nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells? |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 293. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
Anticarsia gemmatalis is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increase use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that midgut is considered one of the most important barriers against viral invasion, before systemic infection can be caused in various tissues if the virus succeeds in reaching the host larvae hemocele. Our work aim to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 oclusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cells were the most affected among the different midgut epithelial cells. In the SL, this cells shows many morphological signs of cellular damage from 24 hours after infection, mainly at the proximal midgut region. However in RL, this cells exhibited minor morphological damages up to 120 hours. Polyhedra were visualized in the midgut of both SL and RL from 96 hours of infection, but the amount of viral structure were always higher in SL, affecting the midgut epithelial cells and tracheal cells, as well as attached hemocytes (mainly plasmatocytes and granulocytes). Our results showed that the AgMNPV, in fact, invade RL cells. However, the virus did not affect these insects as they did with the susceptible ones, allowing development and survival of RL. The mechanism interfering with the virus infection progress in the midgut cells of RL is still unknown. This work has been supported by FAPESP and PRONEX (MCT/Finep/CNPq). MenosAnticarsia gemmatalis is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increase use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that midgut is considered one of the most important barriers against viral invasion, before systemic infection can be caused in various tissues if the virus succeeds in reaching the host larvae hemocele. Our work aim to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 oclusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cells were the most affected among the different midgut epithelial cells. In the SL, this cells shows many morphological signs of cellular... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 03244naa a2200193 a 4500
001 1466838
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aLEVY, S. M.
245 $aIs the nucleopolyhedrovirus of Anticarsia gemmatalis (AgMNPV) ineffective to infect AgMNPV resistant host larva midgut cells?
260 $c2004
300 $ap. 293.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aAnticarsia gemmatalis is a key pests of soybean in Brazil. It has been controlled by a nucleopolyhedrovirus (AgMNPV), which is widely used as a microbial insecticide in the country. The constant and increase use of this biological insecticide in some regions have caused concerns about the possibility of selection of viral resistant populations. Although this phenomenon has not been detected in the field yet, a laboratory A. gemmatalis populations has been selected for high resistance to the AgMNPV. It is known that midgut is considered one of the most important barriers against viral invasion, before systemic infection can be caused in various tissues if the virus succeeds in reaching the host larvae hemocele. Our work aim to verify whether or not the AgMNPV invades and infects the midgut cells of resistant larvae, comparing the ultrastructure of the midgut epithelial cells from the susceptible (SL) and resistant (RL) A. gemmatalis infected larvae. The susceptible and resistant strains of A. gemmatalis were reared on artificial diet, under laboratory-controlled conditions at Embrapa Soja, Londrina-PR, Brazil. The AgMNPV used as inoculum was incorporated into the insect diet at 60,000 oclusion bodies/ml of diet. The midguts were collected up to 120h post infection, processed and analyzed under transmission electron microscopy. The columnar cells were the most affected among the different midgut epithelial cells. In the SL, this cells shows many morphological signs of cellular damage from 24 hours after infection, mainly at the proximal midgut region. However in RL, this cells exhibited minor morphological damages up to 120 hours. Polyhedra were visualized in the midgut of both SL and RL from 96 hours of infection, but the amount of viral structure were always higher in SL, affecting the midgut epithelial cells and tracheal cells, as well as attached hemocytes (mainly plasmatocytes and granulocytes). Our results showed that the AgMNPV, in fact, invade RL cells. However, the virus did not affect these insects as they did with the susceptible ones, allowing development and survival of RL. The mechanism interfering with the virus infection progress in the midgut cells of RL is still unknown. This work has been supported by FAPESP and PRONEX (MCT/Finep/CNPq).
700 1 $aFALLEIROS, A. M. F.
700 1 $aMOSCARDI, F.
700 1 $aGREGÓRIO, E. A.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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