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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
06/04/2010 |
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Data da última atualização: |
06/04/2010 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
COELHO, M. de B.; ARAUJO, F. R.; MATTOSO, L. H. C.; HERRMANN JUNIOR, P. S. de P.; MARCONCINI, J. M.; FRAGOSO, S. P.; LIMA, S. M.; ANDRADE, L. H. da C.; FERREIRA, V. de S.; DELBEN, J. R. J.; DELBEN, A. A. S. T. |
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Afiliação: |
MARLENE DE BARROS COELHO, CNPGC; FLABIO RIBEIRO ARAUJO, CNPGC; LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA; PAULO SERGIO DE P HERRMANN JUNIOR, CNPDIA; JOSE MANOEL MARCONCINI, CNPDIA; Stênio Perdigão Fragoso, INSTITUTO DE BIOLOGIA MOLECULAR DO PARANÁ; Sandro Marcio Lima, UEMS; Luis Humberto da Cunha Andrade, UEMS; Valdir de Souza Ferreira, UFMS; José Renato Jurkevicz Delben, UFMS; Angela Antonia Sanches T. Delben, UFMS. |
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Título: |
Nanotechnology applied to the development of immunosensor for detection of antibodies against Anaplasma marginale. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. |
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Páginas: |
2 p. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
The interest on immunosensors has been increasing because of their utility as specific, simple, rapid, label-free, direct detection techniques with small sample volumes compared with conventional immunoassays. For immunosensors, the immunologic materials are immobilized on a solid supporting material. The immobilized biomolecule retains their bioactivity and interact with their counterparts, and based on the electrochemical detection, the amount or concentration of analytes can be determined. One challenge related to this application is that the reaction of antibody and the antigen-immobilized surface is often insufficient to produce a large electrochemical signal change. It has been showed that the employment of nanoparticles in the building of modified electrodes helps the amplification of the transducing signals of biomolecules interactions, like antigen-antibody. The emergence of nanotechnology has opened new opportunities for the application of nanoparticles in analytical electrochemistry. Additionally, there has been considerable interest in applying immobilized biomolecules (proteins, enzymes, DNA) to specific assays. Among these applications, highly sensitive detection of proteins can be accomplished via antigen-based immunoassays, with the recombinant protein attached to a solid surface. The similar dimensions of nanoparticles and protein molecules enable the enormous electrochemical signal enhancement in a biosensing device. The nanoparticle-biomolecule assemblies provide the basis for ultrasensitive electrochemical biodetection as on impedimetric transducers. In the development of the immunosensor, object of this work, a nanostructured solid surface has been built with the ability to couple the MSP5, recombinant protein from Anaplasma marginale and its correspondent antibody. Screen-printed gold electrodes are used for this purpose and gold nanoparticles layers are mounted on them with the aim of amplifying the impedance detection, or electrochemical signal. MenosThe interest on immunosensors has been increasing because of their utility as specific, simple, rapid, label-free, direct detection techniques with small sample volumes compared with conventional immunoassays. For immunosensors, the immunologic materials are immobilized on a solid supporting material. The immobilized biomolecule retains their bioactivity and interact with their counterparts, and based on the electrochemical detection, the amount or concentration of analytes can be determined. One challenge related to this application is that the reaction of antibody and the antigen-immobilized surface is often insufficient to produce a large electrochemical signal change. It has been showed that the employment of nanoparticles in the building of modified electrodes helps the amplification of the transducing signals of biomolecules interactions, like antigen-antibody. The emergence of nanotechnology has opened new opportunities for the application of nanoparticles in analytical electrochemistry. Additionally, there has been considerable interest in applying immobilized biomolecules (proteins, enzymes, DNA) to specific assays. Among these applications, highly sensitive detection of proteins can be accomplished via antigen-based immunoassays, with the recombinant protein attached to a solid surface. The similar dimensions of nanoparticles and protein molecules enable the enormous electrochemical signal enhancement in a biosensing device. The nanoparticle-biomolecule assemblies ... Mostrar Tudo |
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Palavras-Chave: |
Nanotecnologia. |
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Thesagro: |
Anaplasma Marginale; Anaplasmose; Sanidade Animal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02977naa a2200301 a 4500
001 1663332
005 2010-04-06
008 2009 bl uuuu u00u1 u #d
100 1 $aCOELHO, M. de B.
245 $aNanotechnology applied to the development of immunosensor for detection of antibodies against Anaplasma marginale.
260 $c2009
300 $a2 p.$c1 CD-ROM.
520 $aThe interest on immunosensors has been increasing because of their utility as specific, simple, rapid, label-free, direct detection techniques with small sample volumes compared with conventional immunoassays. For immunosensors, the immunologic materials are immobilized on a solid supporting material. The immobilized biomolecule retains their bioactivity and interact with their counterparts, and based on the electrochemical detection, the amount or concentration of analytes can be determined. One challenge related to this application is that the reaction of antibody and the antigen-immobilized surface is often insufficient to produce a large electrochemical signal change. It has been showed that the employment of nanoparticles in the building of modified electrodes helps the amplification of the transducing signals of biomolecules interactions, like antigen-antibody. The emergence of nanotechnology has opened new opportunities for the application of nanoparticles in analytical electrochemistry. Additionally, there has been considerable interest in applying immobilized biomolecules (proteins, enzymes, DNA) to specific assays. Among these applications, highly sensitive detection of proteins can be accomplished via antigen-based immunoassays, with the recombinant protein attached to a solid surface. The similar dimensions of nanoparticles and protein molecules enable the enormous electrochemical signal enhancement in a biosensing device. The nanoparticle-biomolecule assemblies provide the basis for ultrasensitive electrochemical biodetection as on impedimetric transducers. In the development of the immunosensor, object of this work, a nanostructured solid surface has been built with the ability to couple the MSP5, recombinant protein from Anaplasma marginale and its correspondent antibody. Screen-printed gold electrodes are used for this purpose and gold nanoparticles layers are mounted on them with the aim of amplifying the impedance detection, or electrochemical signal.
650 $aAnaplasma Marginale
650 $aAnaplasmose
650 $aSanidade Animal
653 $aNanotecnologia
700 1 $aARAUJO, F. R.
700 1 $aMATTOSO, L. H. C.
700 1 $aHERRMANN JUNIOR, P. S. de P.
700 1 $aMARCONCINI, J. M.
700 1 $aFRAGOSO, S. P.
700 1 $aLIMA, S. M.
700 1 $aANDRADE, L. H. da C.
700 1 $aFERREIRA, V. de S.
700 1 $aDELBEN, J. R. J.
700 1 $aDELBEN, A. A. S. T.
773 $tIn: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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