| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
21/02/2018 |
|
Data da última atualização: |
21/02/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SOUZA-FILHO, A. F. de; OSÓRIO, A. L. A. R.; JORGE, K. dos S. G.; ARAUJO, F. R.; VIDAL, C. E. S.; ARAÚJO, C. P.; ALBERTTI, L. A. G.; CAZOLA, D. de O.; FERREIRA NETO, J. S.; HEINEMANN, M. B. |
|
Afiliação: |
Antonio Francisco de Souza-Filho, Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo - USP; Ana Luiza Alves Rosa Osório, Faculdade de Medicina Veterinária e Zootecnia - UFMS; Klaudia dos Santos Gonçalves Jorge, Centro de Ciências Biológicas e da Saúde/Universidade Federal de Mato Grosso do Sul - UFMS; FLABIO RIBEIRO ARAUJO, CNPGC; Carlos Eugênio Soto Vidal, Universidade Federal de Santa Maria - UFSM; Cristina Pires Araújo, Faculdade de Medicina Veterinária e Zootecnia - UFMS; Letícia Alves Gomes Albertti, Faculdade de Medicina Veterinária e Zootecnia - UFMS; Daniela de Oliveira Cazola, Faculdade de Medicina Veterinária e Zootecnia - UFMS; José Soares Ferreira Neto, Faculdade de Medicina Veterinária e Zootecnia/USP; Marcos Bryan Heinemann, Faculdade de Medicina Veterinária e Zootecnia/USP. |
|
Título: |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 37, n. 5, suplemento 2, p. 3719-3726, 2016 |
|
Idioma: |
Inglês |
|
Notas: |
Título em português: Perfis genéticos de Mycobacterium bovis em um rebanho bovino no extremo sul do Brasil. |
|
Conteúdo: |
Mycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%), and one profile with one unique isolate (5.6%). This distinction was achieved with the MIRU 31 primer, which resulted in clustering of two isolates into the same profile, and ETR A, B, and C, which discriminated the isolate with a unique profile. The occurrence of clustered isolates is indicative of recent transmission, whereas isolates with a unique profile suggest reactivation of latent infection. The presence of different M. bovis genotypes in the same herd suggests movement of infected animals or different sources of intra-herd infection. Use of the MIRU-VNTR molecular epidemiology technique in M. bovis isolates obtained from an outbreak of bovine tuberculosis in Rio Grande do Sul state demonstrated the genetic diversity of circulating strains, despite the presence of a predominant group. MenosMycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%), and one profile with one unique isolate (5.6%). This distinction was achieved with the MIRU 31 primer, which resulted in clustering of two isolates into the same profile, and ETR A, B, and C, which discriminated the isolate with a unique profile. The occurrence of clustered isolates is indicative of recent transmission, whereas isolates with a unique profile suggest reactivation of latent infection. The presence of different M. bovis genotypes in the same herd suggests movement of infected animals or different sources of intra-herd infection. Use of the MIRU-VNTR molecular epidemiology technique in M. bovis isolates obtained from an outbreak of bovine tuberculosis in Rio Grande do Sul state demonstrated the genetic diversity ... Mostrar Tudo |
|
Palavras-Chave: |
Diversidade genética; Genetical diversity; MIRU-VNTR; Tuberculose bovina. |
|
Thesagro: |
Mycobacterium Bovis. |
|
Thesaurus Nal: |
Bovine tuberculosis. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/172941/1/28-Genetic-profiles-of-Mycobacterium-bovis-from-a-cattle-herd-in-southernmost-Brazil-2016.pdf
|
|
Marc: |
LEADER 02626naa a2200313 a 4500
001 2087984
005 2018-02-21
008 2016 bl uuuu u00u1 u #d
100 1 $aSOUZA-FILHO, A. F. de
245 $aGenetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil.$h[electronic resource]
260 $c2016
500 $aTítulo em português: Perfis genéticos de Mycobacterium bovis em um rebanho bovino no extremo sul do Brasil.
520 $aMycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%), and one profile with one unique isolate (5.6%). This distinction was achieved with the MIRU 31 primer, which resulted in clustering of two isolates into the same profile, and ETR A, B, and C, which discriminated the isolate with a unique profile. The occurrence of clustered isolates is indicative of recent transmission, whereas isolates with a unique profile suggest reactivation of latent infection. The presence of different M. bovis genotypes in the same herd suggests movement of infected animals or different sources of intra-herd infection. Use of the MIRU-VNTR molecular epidemiology technique in M. bovis isolates obtained from an outbreak of bovine tuberculosis in Rio Grande do Sul state demonstrated the genetic diversity of circulating strains, despite the presence of a predominant group.
650 $aBovine tuberculosis
650 $aMycobacterium Bovis
653 $aDiversidade genética
653 $aGenetical diversity
653 $aMIRU-VNTR
653 $aTuberculose bovina
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. dos S. G.
700 1 $aARAUJO, F. R.
700 1 $aVIDAL, C. E. S.
700 1 $aARAÚJO, C. P.
700 1 $aALBERTTI, L. A. G.
700 1 $aCAZOLA, D. de O.
700 1 $aFERREIRA NETO, J. S.
700 1 $aHEINEMANN, M. B.
773 $tSemina: Ciências Agrárias, Londrina$gv. 37, n. 5, suplemento 2, p. 3719-3726, 2016
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 267 | |
| 3. |  | MATOS, A. P. de. Doenças do abacaxizeiro. Informe Agropecuário, Belo Horizonte, v.37, n.290, p.7-15, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|    |
| 4. | | MATOS, A. P. de. Doenças e seu controle. In: REINHARDT, D. H. R. C.; SOUZA, L. F. da S.; CABRAL, J. R. S. (Ed.). Abacaxi : produção : aspectos técnicos. 2. ed. rev. e atual. Brasília, DF : Embrapa, 2014. E-book : il.; (Frutas do Brasil; 27).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 10. | | MATOS, A. P. de. Produção integrada do abacaxi no Brasil. In: SEMANA INTERNACIONAL DA FRUTICULTURA, FLORICULTURA E AGROINDÚSTRIA, 14., 2007. Frutal 2007: agronegócio e responsabilidade social [anais]. Fortaleza: Instituto Frutal, 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 11. | | MATOS, A. P. de. Produção Integrada de Abacaxi. In: MATOS, A. P. de; BORGES, A. L.; OLIVEIRA, D. de A.; SANTOS, G. R. dos; CAMPOS, G. A.; GEBLER, L.; SANCHES, N. F.; SOUTO, R. F.; CORDEIRO, Z. J. M. (Ed.). Produção integrada de fruteiras tropicais. Cruz das Almas: Embrapa Mandioca e Fruticultura, 2012. p.25-68. Publicação online.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 12. | | MATOS, A. P. de (ed.). Abacaxi fitossanidade. 2. ed. rev. e atual. Brasília, DF : Embrapa, 2017. il. color. E-book : il. color. (Embrapa. Série Frutas do Brasil, 9).| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 20. | | JUNGHANS, D. T.; MATOS, A. P. de. Pineapple germplasm and Fusariosis resistance. Pineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S. Disponível em: < http://www.ishs-horticulture.org/workinggroups/pineapple/PineNews17_addendum.pdf> Acesso em: 16 fev. 2011. Edição de Abstracts of the 7th International Pineapple Symposium, Johor Baru, Malaysia, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| Registros recuperados : 267 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
