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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/12/2010 |
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Data da última atualização: |
09/02/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ABREU, E. F. M.; JESUS, C. de J.; ANDRADE, E. C. de; ARAGAO, F. J. L.; VIANNA, G. R. |
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Afiliação: |
EMANUEL FELIPE MEDEIROS ABREU, CNPMF; CAMILA C. de JESUS, UFRB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN; GIOVANNI RODRIGUES VIANNA, CENARGEN. |
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Título: |
Genetic transformation of Carica papaya to obtaining tolerance to abiotc stress. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Anais... Fortaleza: Sociedade Brasileira de Biotecnologia. |
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Páginas: |
338 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Due to a new climatic reality that we have together , which causes scarcity of water resources, increased salinity and constant temperature change, increasingly we to obtain plants able to adapt to abiotic stresses, which have a great impact on their growth and productivity. To minimize the adverse effects caused by theses factors, the plant starts production of molecules involved in response and is essential for their survival, as HSPs (heat shock proteins). These molecules are responsible for protein folding, assembly, translocation and degradation in a broad array of normal cellular process; they also function in the stabilization of proteins and membranes, and can assist in protein refolding under stress conditions (Wang et al., 2004). The paper aims is generate transgenic C. papaya expressing sHsps obtained from Musa acuminata leaves subjected to heat and e cold, and evaluate the correlation betwen the response to abiotic stresses with the differential expression of these proteins. For genetic transformation, green fruit open pollinated "Sunrise" papaya, harvested 90 to 120 days after fruit set, was obtained, from germplasm bank from Embrapa Cassava and Tropical Fruit. Immature zygotic embryos were surface-sterilized with 70% ethanol followed by immersion in 1% sodium hypochlorite for 20 min and then rinsed three times in sterile water. The embryos were placed on induction medium that was the same as that reported by Fitch et al. (1990). For each bombardment test higly embryogenic cultures were selected and about 13-15 somatic embryo clumps were spread onto a filter paper. Thus three vectores (pUCBAr 194, pUCBar163 and pUCBar129) were constructed. Each vector constructed one different sHsps genes, the Bar gene selection gene and sGFP gene, all under control of the CaMV 35s promoter. After each bombarded the embryos were then transferred onto intuction medium (Ficth et al. 1990) incubated for 2-3 weeks in the dark at 28. C. To present an initial analysis performed 7 days after bombardment; a small tissue sample from each plate was tested for transient sGFP expression. Observatiion under a dissecting microscope revealed numerous spots in simples from DNA-bombarded plates. MenosDue to a new climatic reality that we have together , which causes scarcity of water resources, increased salinity and constant temperature change, increasingly we to obtain plants able to adapt to abiotic stresses, which have a great impact on their growth and productivity. To minimize the adverse effects caused by theses factors, the plant starts production of molecules involved in response and is essential for their survival, as HSPs (heat shock proteins). These molecules are responsible for protein folding, assembly, translocation and degradation in a broad array of normal cellular process; they also function in the stabilization of proteins and membranes, and can assist in protein refolding under stress conditions (Wang et al., 2004). The paper aims is generate transgenic C. papaya expressing sHsps obtained from Musa acuminata leaves subjected to heat and e cold, and evaluate the correlation betwen the response to abiotic stresses with the differential expression of these proteins. For genetic transformation, green fruit open pollinated "Sunrise" papaya, harvested 90 to 120 days after fruit set, was obtained, from germplasm bank from Embrapa Cassava and Tropical Fruit. Immature zygotic embryos were surface-sterilized with 70% ethanol followed by immersion in 1% sodium hypochlorite for 20 min and then rinsed three times in sterile water. The embryos were placed on induction medium that was the same as that reported by Fitch et al. (1990). For each bombardment test h... Mostrar Tudo |
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Palavras-Chave: |
Papaya; Transformação genética. |
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Thesagro: |
Mamão. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02889naa a2200217 a 4500
001 1868781
005 2011-02-09
008 2010 bl uuuu u00u1 u #d
100 1 $aABREU, E. F. M.
245 $aGenetic transformation of Carica papaya to obtaining tolerance to abiotc stress.
260 $c2010
300 $a338 p.
520 $aDue to a new climatic reality that we have together , which causes scarcity of water resources, increased salinity and constant temperature change, increasingly we to obtain plants able to adapt to abiotic stresses, which have a great impact on their growth and productivity. To minimize the adverse effects caused by theses factors, the plant starts production of molecules involved in response and is essential for their survival, as HSPs (heat shock proteins). These molecules are responsible for protein folding, assembly, translocation and degradation in a broad array of normal cellular process; they also function in the stabilization of proteins and membranes, and can assist in protein refolding under stress conditions (Wang et al., 2004). The paper aims is generate transgenic C. papaya expressing sHsps obtained from Musa acuminata leaves subjected to heat and e cold, and evaluate the correlation betwen the response to abiotic stresses with the differential expression of these proteins. For genetic transformation, green fruit open pollinated "Sunrise" papaya, harvested 90 to 120 days after fruit set, was obtained, from germplasm bank from Embrapa Cassava and Tropical Fruit. Immature zygotic embryos were surface-sterilized with 70% ethanol followed by immersion in 1% sodium hypochlorite for 20 min and then rinsed three times in sterile water. The embryos were placed on induction medium that was the same as that reported by Fitch et al. (1990). For each bombardment test higly embryogenic cultures were selected and about 13-15 somatic embryo clumps were spread onto a filter paper. Thus three vectores (pUCBAr 194, pUCBar163 and pUCBar129) were constructed. Each vector constructed one different sHsps genes, the Bar gene selection gene and sGFP gene, all under control of the CaMV 35s promoter. After each bombarded the embryos were then transferred onto intuction medium (Ficth et al. 1990) incubated for 2-3 weeks in the dark at 28. C. To present an initial analysis performed 7 days after bombardment; a small tissue sample from each plate was tested for transient sGFP expression. Observatiion under a dissecting microscope revealed numerous spots in simples from DNA-bombarded plates.
650 $aMamão
653 $aPapaya
653 $aTransformação genética
700 1 $aJESUS, C. de J.
700 1 $aANDRADE, E. C. de
700 1 $aARAGAO, F. J. L.
700 1 $aVIANNA, G. R.
773 $tIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Anais... Fortaleza: Sociedade Brasileira de Biotecnologia.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 343 | |
| 165. |  | JESUS, C. C.; ARAGAO, F. J. L.; ANDRADE, E. C. de; VIANNA, G. R.; ABREU, E. F. M. Estabelecimento de um protocolo de regeneração por embriogênse somática de Carica Papaya L. VAR. Sunrise. In: REUNIÃO ANUAL DE CIÊNCIA, TECNOLOGIA, INOVAÇÃO E CULTURA NO RECÔNCAVO DA BAHIA - RECITEC RECÔNCAVO. 1., 2011, Cruz das Almas. Anais... Cruz das Almas: Universidade Federal do Recôncavo da Bahia, 2011. 1 CD-ROM. 2 p. Em paralelo aconteceram também os seguintes eventos: V Seminário de Pesquisa do Recôncavo da Bahia; V Seminário Estudantil de Pesquisa da UFRB; V Seminário da Pós-Graduação da UFRB; II Seminário Regional de Pesquisa da EBDA; 5ª Jornada...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 167. | | FARIA, J. C.; BONFIM, K.; NOGUEIRA, E. O. P. L.; MENDES, E. A.; ARAGÃO, F. J. L. Resistance to bean golden mosaic virus in genetically engineered common bean mediated by RNAi. In: ANNIVERSARY OF THE BEAN IMPROVEMENT COOPERATIVE, 50.; BIENNIAL, 19., 2007, Madison, Wisconsin. Program and abstracts. [S. l.]: Bean Improvement Cooperative, 2007. p. 15.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 168. | | FARIA, J. C.; BONFIM, K.; NOGUEIRA, E. O. P. L.; MENDES, E. A.; ARAGÃO, F. J. L. Resistance to bean golden mosaic virus in genetically engineered common bean mediated by RNAi. In: ANNIVERSARY OF THE BEAN IMPROVEMENT COOPERATIVE, 50.; BIENNIAL, 19., 2007, Madison, Wisconsin. Program and abstracts. [S. l.]: Bean Improvement Cooperative, 2007. p. 15. Oral presentations.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 170. | | JESUS, C. C. de; ARAGÃO, F. J. L; ANDRADE, E. C. de; VIANNA, G. R.; ABREU, E. F. M. Seleção de linhagens transgênicas NICOTIANA TABACUM L. expressando SMALL HEAT SHOCK PROTEINS (SHSP) isolados de bananeira (MUSA SP.). In: REUNIÃO ANUAL DE CIÊNCIA, TECNOLOGIA, INOVAÇÃO E CULTURA NO RECÔNCAVO DA BAHIA - RECONCITEC, 2., 2012, Cruz das Almas. Energias Renováveis, Educação, Tecnologia e Sociedade. Cruz das Almas, BA: UFRB, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 171. | | TINOCO, M. L.; VIANNA, G. R.; ABUD, S.; SOUZA, P. I. M.; RECH FILHO, E. L.; ARAGÃO, F. J. L. Radiation as a tool to remove selective marker genes from transgenic soybean plants. Biologia Plantarum, v. 50, n. 1, p. 146-148, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 172. | | TINOCO, M. L.; VIANNA, G. R.; ABUD, S.; SOUZA, P. I. M.; RECH, E. L.; ARAGÃO, F. J. L. Radiation as a tool to remove selective marker genes from transgenic soybean plants. Biologia Plantarum, v. 50, n. 1, p. 146-148, 2006.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 175. | | CIPRIANO, T. de M.; CARVALHO, L. J. C. B.; CARNEIRO, N. P.; CARNEIRO, A. A.; ARAGÃO, F. J. L. Silenciamento mediado por RNA interferente do gene BE1 que codifica para a enzima de ramificação do amido I (SBE I) em milho (Zea mays L.). Revista Brasileira de Horticultura Ornamental, Campinas, v. 13, p. 329, 2007. Suplemento. Edição de Anais do 16º Congresso Brasileiro de Floricultura e Plantas Ornamentais; 3º Congresso Brasileiro de Cultura de Tecidos de Plantas; 1º Simpósio de Plantas Ornamentais Nativas, Goiânia, nov. 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 176. | | CIPRIANO, T. de M.; CARVALHO, L. J. C. B.; CARNEIRO, N. P.; CARNEIRO, A. A.; ARAGÃO, F. J. L. Silenciamento mediado por RNA interferente do gene be1 que codifica para a enzima de ramifricação do amido I (SBE I) em milho (Zea mays L.). Revista Brasileira de Horticultura Ornamental, Campinas, v. 13, supl., p. 329, 2007. Edição dos Resumos do XVI Congresso Brasileiro de Floricultura e Plantas Ornamentais; III Congresso Brasileiro de Cultura de Tecidos e Plantas; I Simpósio de Plantas Ornamentais Nativas, Goiânia, set. 2007. Disponível também em: ENCONTRO...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 179. | | BONFIM, K.; FARIA, J. C.; NOGUEIRA, E. O. P. L.; MENDES, E. A.; ARAGÃO, F. J. L. RNAi-mediated resistance to Bean golden mosaic virus genetically engineered common bean. In: INTERNATIONAL GEMINIVIRUS SYMPOSIUM, 5.; INTERNATIONAL SSDNA COMPARATIVE VIROLOGY WORKSHOP, 3., 2007, Ouro Preto. Program e abstracts. Viçosa, MG: Universidade Federal de Viçosa, 2007. p. 55.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 180. | | BONFIM, K.; FARIA, J. C. de; NOGUEIRA, E. O. P. L.; MENDES, E. A.; ARAGÃO, F. J. L. RNAi-mediated resistance to Bean golden mosaic virus genetically engineered common bean. In: INTERNATIONAL GEMINIVIRUS SYMPOSIUM, 5.; INTERNATIONAL SSDNA COMPARATIVE VIROLOGY WORKSHOP, 3., 2007, Ouro Preto. Program e abstracts. Viçosa, MG: Universidade Federal de Viçosa, 2007. p. 55.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 343 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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