Data/hora: 21/09/2024 04:02:49

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	26/12/2001	
Data da última atualização: 	14/01/2020	
Autoria: 	FARIA, J. C.; ALBINO, M. M. C.; DIAS, B. B. A.; VIANNA, M. G. R.; ARAGÃO, F. J. L.	
Afiliação: 	JOSIAS CORREA DE FARIA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen.	
Título: 	Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado.	
Ano de publicação: 	2001	
Fonte/Imprenta: 	In: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOTECNOLOGIA, 1., 2001, São Paulo. [Resumos expandidos]... Brasilia: SBBIOTEC, 2001.	
Idioma: 	Português	
Conteúdo: 	Tbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless.	
Palavras-Chave: 	BGMV; Biobalística; Tolerância; Transformação.	
Thesagro: 	Feijão; Mosaico Dourado; Phaseolus Vulgaris; Vírus.	
Thesaurus Nal: 	Bean golden mosaic virus; mutation.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/208710/1/feijoeiro-transgenico0001.pdf	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (18085-1) - UPC - AA - DD - 2001 -  - 2001 - DIS