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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/08/2013 |
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Data da última atualização: |
08/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAGÃO, F. J. L.; NOGUEIRA, E. O. P. L.; TINOCO, M. L. P.; FARIA, J. C. |
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Afiliação: |
FRANCISCO JOSE LIMA ARAGAO, CENARGEN; ELSA OLIVEIRA P E LAGO NOGUEIRA, CENARGEN; MARIA LAINE P. TINOCO, CENARGEN; JOSIAS CORREA DE FARIA, CNPAF. |
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Título: |
Molecular characterization of the first commercial transgenic common bean immune to the Bean golden mosaic virus. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Journal of Biotechnology, Amsterdam, v. 66, n. 1/2, p. 42-50, June 2013. |
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DOI: |
http://dx.doi.org/10.1016/j.jbiotec.2013.04.009 |
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Idioma: |
Inglês |
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Conteúdo: |
Golden mosaic of common bean is caused by the Bean golden mosaic virus (BGMV). The disease is one of the greatest constraints on bean production in Latin America and causes significant yield losses. The RNAi concept was explored to silence the rep (AC1) viral gene and a transgenic bean line immune to BGMV upon inoculation at high pressure was previously generated. Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene. It is flanked by Phaseolus genomic sequences and interspersed by one nuclear and three chloroplastic genomic sequences. Southern analyses showed that the transgenes were structurally stable for eight self-pollinated generations and after backcrosses with a non transgenic commercial variety. Transgene expression analyses revealed similar levels of siRNA in leaves of transgenic plants cultivated under field conditions in three distinct regions. siRNA were also analyzed during seed development in common bean transgenic plants. siRNA signals were also detected in seeds, albeit at significantly lower levels than those observed in leaves, and could not be detected in seeds cooked during 10 min. This information is relevant to demonstrate that GM beans are free of siRNA signals after cooking and therefore suitable for human consumption. Additionally, characterization of the locus where the transgene was integrated in the common bean genome provides a valuable tool to trace this GM bean material in the field and in the market. MenosGolden mosaic of common bean is caused by the Bean golden mosaic virus (BGMV). The disease is one of the greatest constraints on bean production in Latin America and causes significant yield losses. The RNAi concept was explored to silence the rep (AC1) viral gene and a transgenic bean line immune to BGMV upon inoculation at high pressure was previously generated. Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene. It is flanked by Phaseolus genomic sequences and interspersed by one nuclear and three chloroplastic genomic sequences. Southern analyses showed that the transgenes were structurally stable for eight self-pollinated generations and after backcrosses with a non transgenic commercial variety. Transgene expression analyses revealed similar levels of siRNA in leaves of transgenic plants cultivated under field conditions in three distinct regions. siRNA were also analyzed during seed development in common bean transgenic plants. siRNA signals were also detected in seeds, albeit at significantly lower levels than those observed in leaves, and could not be detected in seeds cooked during 10 min. This information is relevant to demonstrate that GM beans are free of siRNA signals after cooking and therefore suitable for human consumption. Additionally, characterization of the locus where the transgene was integrated in the ... Mostrar Tudo |
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Thesagro: |
Engenharia genética; Feijão. |
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Thesaurus Nal: |
Begomovirus; Dry beans; Geminiviridae; Genetic engineering; RNA interference. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02424naa a2200253 a 4500
001 1968340
005 2023-03-08
008 2013 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.jbiotec.2013.04.009$2DOI
100 1 $aARAGÃO, F. J. L.
245 $aMolecular characterization of the first commercial transgenic common bean immune to the Bean golden mosaic virus.$h[electronic resource]
260 $c2013
520 $aGolden mosaic of common bean is caused by the Bean golden mosaic virus (BGMV). The disease is one of the greatest constraints on bean production in Latin America and causes significant yield losses. The RNAi concept was explored to silence the rep (AC1) viral gene and a transgenic bean line immune to BGMV upon inoculation at high pressure was previously generated. Identification of the transgene insert confirmed the presence of a single locus corresponding to two intact copies of the RNAi cassette in opposite orientation and three intact copies of the AtAhas gene. It is flanked by Phaseolus genomic sequences and interspersed by one nuclear and three chloroplastic genomic sequences. Southern analyses showed that the transgenes were structurally stable for eight self-pollinated generations and after backcrosses with a non transgenic commercial variety. Transgene expression analyses revealed similar levels of siRNA in leaves of transgenic plants cultivated under field conditions in three distinct regions. siRNA were also analyzed during seed development in common bean transgenic plants. siRNA signals were also detected in seeds, albeit at significantly lower levels than those observed in leaves, and could not be detected in seeds cooked during 10 min. This information is relevant to demonstrate that GM beans are free of siRNA signals after cooking and therefore suitable for human consumption. Additionally, characterization of the locus where the transgene was integrated in the common bean genome provides a valuable tool to trace this GM bean material in the field and in the market.
650 $aBegomovirus
650 $aDry beans
650 $aGeminiviridae
650 $aGenetic engineering
650 $aRNA interference
650 $aEngenharia genética
650 $aFeijão
700 1 $aNOGUEIRA, E. O. P. L.
700 1 $aTINOCO, M. L. P.
700 1 $aFARIA, J. C.
773 $tJournal of Biotechnology, Amsterdam$gv. 66, n. 1/2, p. 42-50, June 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 340 | |
| 101. |  | NICKEL, O.; SILVA, S. W.; FAJARDO, T. V. M.; GIACOMINI, R.; ARAGAO, F. J. L. Detecção e caracterização molecular do gene da proteína capsidial de apple mosaic virus em macieiras. Tropical Plant Pathology, Brasília, DF, v. 34, p. S267, 2009. Resumo 900.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 105. | | RADAELLI, P.; NICKEL, O.; ARAGÃO, F. J. L.; FAJARDO, T. V. M.; SCHONS, J. Diagnóstico biológico e molecular e análise da sequência de nucleotídios do gene de capa protéica de um isolado de apple stem pitting virus. Fitopatologia Brasileira, Brasília, DF, v. 29, p. S239, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 119. | | FARIA, J. C.; VALDISSER, P. A. M. R.; DEL PELOSO, M. J.; ARAGÃO, F. J. L. Linhagem de feijoeiro expressando RNA interferente é resistente ao mosaico dourado e transmite o transgene às progênies derivadas por retrocruzamento. Tropical Plant Pathology, v. 34, p. S268, ago. 2009. Suplemento. Ref. 904. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, ago. 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 340 | |
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