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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/02/2012 |
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Data da última atualização: |
27/02/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ABREU, E. F. M.; ANDRADE, E. C. de; ARAGAO, F. J. L. |
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Afiliação: |
Emanuel Felipe Medereiros Abreu, UnB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
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Título: |
Molecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. pdf 34699 |
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Páginas: |
p. 6 |
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Idioma: |
Inglês |
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Conteúdo: |
Cowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV. MenosCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively,... Mostrar Tudo |
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Palavras-Chave: |
CABMV; Cowpea; CpSMV; Molecular and RT-PCR analysis; Virus diseases. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02995nam a2200205 a 4500
001 1915005
005 2023-02-27
008 2011 bl uuuu u01u1 u #d
100 1 $aABREU, E. F. M.
245 $aMolecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. pdf 34699$c2011
300 $ap. 6
520 $aCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV.
653 $aCABMV
653 $aCowpea
653 $aCpSMV
653 $aMolecular and RT-PCR analysis
653 $aVirus diseases
700 1 $aANDRADE, E. C. de
700 1 $aARAGAO, F. J. L.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
12/08/2010 |
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Data da última atualização: |
15/04/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
LIMA, D.; BRUEL, U. L.; CARNEIRO, G. E. S.; SILVA FILHO, P. M.; DOMIT, L. A.; OLIVEIRA, A. B.; PIPOLO, A. E.; PETEK, M. R.; BORGES, R. S.; MIRANDA, L. C.; FERREIRA, C. C.; BAIL, J. L.; BECKERT, O. P.; DENGLER, R. U.; GOMIDE, F. B.; DALBOSCO, M.; OLIVEIRA, W. J. S.; AZAMBUJA, J. R. S. |
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Afiliação: |
DIVANIA DE LIMA, CNPSo; UBIRAJARA LUIZ BRUEL, CNPSo; GERALDO ESTEVAM DE SOUZA CARNEIRO, CNPSo; PEDRO MOREIRA DA SILVA FILHO, CNPSo; LINEU ALBERTO DOMIT, CPAMT; ARNOLD BARBOSA DE OLIVEIRA, CNPSo; ANTONIO EDUARDO PIPOLO, CNPSo; MARCOS RAFAEL PETEK, SNTEEN Londrina; ROGERIO DE SA BORGES, SNTEEN Londrina; LUIZ CARLOS MIRANDA, SNTEEN Londrina; CAIRO CEZAR FERREIRA, SNTEEN Londrina; JOSE LUCIANO BAIL, SNTEEN P Grossa; OSMAR PAULO BECKERT, SNTEEN P Grossa; R. U. DENGLER, Fundação Meridional de Apoio a Pesquisa Agropecuária; F. B. GOMIDE, Fundação Meridional de Apoio a Pesquisa Agropecuária; M. DALBOSCO, Fundação Meridional de Apoio a Pesquisa Agropecuária; W. J. S. OLIVEIRA, Fundação Meridional de Apoio a Pesquisa Agropecuária; J. R. S. AZAMBUJA, Fundação Meridional de Apoio a Pesquisa Agropecuária. |
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Título: |
A utilização de dias de campo como ferramenta do processo de transferência de tecnologia. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 31., 2010, Brasília, DF. Resumos... Londrina: Embrapa Soja, 2010. p. 19-20. Editores técnicos: Adilson de Oliveira Junior, Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campo Leite, César de Castro, Jussara Flores de Oliveira Arbues, Wellington Cavalcanti. |
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Idioma: |
Português |
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Thesagro: |
Soja; Transferência de tecnologia. |
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Thesaurus NAL: |
Soybeans; Technology transfer. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01282naa a2200361 a 4500
001 1859837
005 2011-04-15
008 2010 bl uuuu u00u1 u #d
100 1 $aLIMA, D.
245 $aA utilização de dias de campo como ferramenta do processo de transferência de tecnologia.
260 $c2010
650 $aSoybeans
650 $aTechnology transfer
650 $aSoja
650 $aTransferência de tecnologia
700 1 $aBRUEL, U. L.
700 1 $aCARNEIRO, G. E. S.
700 1 $aSILVA FILHO, P. M.
700 1 $aDOMIT, L. A.
700 1 $aOLIVEIRA, A. B.
700 1 $aPIPOLO, A. E.
700 1 $aPETEK, M. R.
700 1 $aBORGES, R. S.
700 1 $aMIRANDA, L. C.
700 1 $aFERREIRA, C. C.
700 1 $aBAIL, J. L.
700 1 $aBECKERT, O. P.
700 1 $aDENGLER, R. U.
700 1 $aGOMIDE, F. B.
700 1 $aDALBOSCO, M.
700 1 $aOLIVEIRA, W. J. S.
700 1 $aAZAMBUJA, J. R. S.
773 $tIn: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 31., 2010, Brasília, DF. Resumos... Londrina: Embrapa Soja, 2010. p. 19-20. Editores técnicos: Adilson de Oliveira Junior, Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campo Leite, César de Castro, Jussara Flores de Oliveira Arbues, Wellington Cavalcanti.
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