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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/04/1994 |
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Data da última atualização: |
25/04/1994 |
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Autoria: |
CABRAL, G. B.; ARAGAO, F. J. L.; MONTE-NESHICH, D.C.; RECH, E. L. |
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Afiliação: |
CERNARGEN-EMBRAPA. |
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Título: |
Transient gene expression in cassava protoplasts . |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Cali, Colombia: CIAT, 1993 |
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Páginas: |
244-250p |
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Série: |
CIAT, Wording Document, 123 |
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Idioma: |
Inglês |
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Notas: |
Cali, Colombia: CIAT, 1993. |
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Conteúdo: |
The aim of the project is the development and improvement of methodologies for gene tranfer in cassava. Isoloted protoplasts provide a convenient system for studying the paramenters influencing the transformation of protoplasts by firect DNA uptake. Transient expression studies have utilized a plasmid pAL carrying the beta-glucoronidase gene under control of 35S-CaMV promoter and nos polyadenilation region, following electrporation-mediated plasmid uptake. Protoplasts were isolated from cassave leaves (var. MCol22) growing in vitro, resluspended iln elevtroporation buffer (20 mM MES, pH 5.8, 10 mM CaCI2) and 20 mug ml-1 plasmid. A viability culrve of the optimum electroporation parameter gave an electric field of 715 V.cm-1 and a tilme constant =12.8 msec. The expression of the gene product was analyzed by a qualitative and quantitative beta-glucuronidase enzyme assay. The results obtained from the foundation to study direct gene transfer in cassava. In addition, may allow a rapid method to evaluate the functional expression of tissue-specific promoters. Nevertheless, experiments have been carried out utilizing particle bombardment using tissues with regeneration capability. The production of transgenic plants shoud facilitate, in the future, studies to the introduction and expression of important agronomically traits in cassava. |
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Palavras-Chave: |
Congresso; Maninhot esculenta; Producao: Alimentacao. |
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Thesagro: |
Melhoramento. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01992naa a2200241 a 4500
001 1644385
005 1994-04-25
008 1993 bl uuuu u00u1 u #d
100 1 $aCABRAL, G. B.
245 $aTransient gene expression in cassava protoplasts .
260 $c1993
300 $a244-250p
490 $aCIAT, Wording Document, 123
500 $aCali, Colombia: CIAT, 1993.
520 $aThe aim of the project is the development and improvement of methodologies for gene tranfer in cassava. Isoloted protoplasts provide a convenient system for studying the paramenters influencing the transformation of protoplasts by firect DNA uptake. Transient expression studies have utilized a plasmid pAL carrying the beta-glucoronidase gene under control of 35S-CaMV promoter and nos polyadenilation region, following electrporation-mediated plasmid uptake. Protoplasts were isolated from cassave leaves (var. MCol22) growing in vitro, resluspended iln elevtroporation buffer (20 mM MES, pH 5.8, 10 mM CaCI2) and 20 mug ml-1 plasmid. A viability culrve of the optimum electroporation parameter gave an electric field of 715 V.cm-1 and a tilme constant =12.8 msec. The expression of the gene product was analyzed by a qualitative and quantitative beta-glucuronidase enzyme assay. The results obtained from the foundation to study direct gene transfer in cassava. In addition, may allow a rapid method to evaluate the functional expression of tissue-specific promoters. Nevertheless, experiments have been carried out utilizing particle bombardment using tissues with regeneration capability. The production of transgenic plants shoud facilitate, in the future, studies to the introduction and expression of important agronomically traits in cassava.
650 $aMelhoramento
653 $aCongresso
653 $aManinhot esculenta
653 $aProducao: Alimentacao
700 1 $aARAGAO, F. J. L.
700 1 $aMONTE-NESHICH, D.C.
700 1 $aRECH, E. L.
773 $tCali, Colombia: CIAT, 1993
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
05/08/2010 |
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Data da última atualização: |
07/12/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ROSISCA, J. R.; GONÇALVES, S. L.; GRUNVALD, A. K.; OLIVEIRA, M. C. N. de. |
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Afiliação: |
JULIANDRA RODRIGUES ROSISCA, UNIFIL; SERGIO LUIZ GONÇALVES, CNPSo; ANNA KAROLINA GRUNVALD, UEM; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSo. |
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Título: |
Quebra de dormência em sementes de girassol silvestre utilizando ácido giberélico. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 98-101. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo. |
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Idioma: |
Português |
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Palavras-Chave: |
Girassol silvestre. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/71810/1/ID-30971.pdf
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Marc: |
LEADER 00621nam a2200145 a 4500
001 1859345
005 2012-12-07
008 2010 bl uuuu u00u1 u #d
100 1 $aROSISCA, J. R.
245 $aQuebra de dormência em sementes de girassol silvestre utilizando ácido giberélico.
260 $aIn: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 98-101. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.$c2010
653 $aGirassol silvestre
700 1 $aGONÇALVES, S. L.
700 1 $aGRUNVALD, A. K.
700 1 $aOLIVEIRA, M. C. N. de
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Embrapa Soja (CNPSO) |
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