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Registro Completo |
Biblioteca(s): |
Embrapa Algodão; Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
15/09/2022 |
Data da última atualização: |
16/09/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERREIRA, A. L.; FARIA, J. C. de; MOURA, M. da C.; ZAIDEM, A. L. de M.; PIZETTA, C. S. R.; FREITAS, E. de O.; COELHO, G. R. C.; SILVA, J. F. A. e; BARRIGOSSI, J. A. F.; HOFFMANN, L. V.; SOUZA, T. L. P. O. de; ARAGÃO, F. J. L.; PINHEIRO, P. V. |
Afiliação: |
AMANDA LOPES FERREIRA, bolsista CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MATHEUS DA COSTA MOURA, UNIVERSIDADE FEDERAL DE GOIÁS; ANTÔNIA LOPES DE MENDONÇA ZAIDEM, UNIVERSIDADE FEDERAL DE GOIÁS; CAROLINA SENHORINHO RAMALHO PIZETTA; ELÍNEA DE OLIVEIRA FREITAS; GESIMARIA RIBEIRO COSTA COELHO, CNPAF; JOSE FRANCISCO ARRUDA E SILVA, CNPAF; JOSE ALEXANDRE F BARRIGOSSI, CNPAF; LUCIA VIEIRA HOFFMANN, CNPA; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen; PATRICIA VALLE PINHEIRO, CNPAF. |
Título: |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Frontiers in Plant Science, v. 13, 984804, Aug. 2022. |
ISSN: |
1664-462X |
DOI: |
https://doi.org/10.3389/fpls.2022.984804 |
Idioma: |
Inglês |
Conteúdo: |
Common bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases. MenosCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays we... Mostrar Tudo |
Palavras-Chave: |
Dry bean; Insect pest management; VATPase. |
Thesagro: |
Bemisia Tabaci; Feijão; Mosca Branca; Phaseolus Vulgaris; Planta Transgênica. |
Thesaurus Nal: |
Beans; RNA interference; Transgenic plants. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146488/1/fpls-984804.pdf
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Marc: |
LEADER 03529naa a2200421 a 4500 001 2146488 005 2022-09-16 008 2022 bl uuuu u00u1 u #d 022 $a1664-462X 024 7 $ahttps://doi.org/10.3389/fpls.2022.984804$2DOI 100 1 $aFERREIRA, A. L. 245 $aWhitefly-tolerant transgenic common bean (Phaseolus vulgaris) line.$h[electronic resource] 260 $c2022 520 $aCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases. 650 $aBeans 650 $aRNA interference 650 $aTransgenic plants 650 $aBemisia Tabaci 650 $aFeijão 650 $aMosca Branca 650 $aPhaseolus Vulgaris 650 $aPlanta Transgênica 653 $aDry bean 653 $aInsect pest management 653 $aVATPase 700 1 $aFARIA, J. C. de 700 1 $aMOURA, M. da C. 700 1 $aZAIDEM, A. L. de M. 700 1 $aPIZETTA, C. S. R. 700 1 $aFREITAS, E. de O. 700 1 $aCOELHO, G. R. C. 700 1 $aSILVA, J. F. A. e 700 1 $aBARRIGOSSI, J. A. F. 700 1 $aHOFFMANN, L. V. 700 1 $aSOUZA, T. L. P. O. de 700 1 $aARAGÃO, F. J. L. 700 1 $aPINHEIRO, P. V. 773 $tFrontiers in Plant Science$gv. 13, 984804, Aug. 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
29/11/1993 |
Data da última atualização: |
28/08/2024 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
MELO, C. F. M. de; GOMES, J. I. |
Afiliação: |
CELIO FRANCISCO MARQUES DE MELO, CPATU; JOAQUIM IVANIR GOMES, CPATU. |
Título: |
Estudo químico e micrográfico de madeiras da Amazônia. |
Ano de publicação: |
1979 |
Fonte/Imprenta: |
Belém, PA: EMBRAPA-CPATU, 1979. |
Páginas: |
70 p. |
Descrição Física: |
il. |
Série: |
(EMBRAPA-CPATU. Comunicado técnico, 19). |
Idioma: |
Português |
Conteúdo: |
Introdução. Material e métodos. Análises químicas. Estudo micrográfico. Resultados. Características micrográficas das fibras. Discussão e conclusões. Anexos. |
Palavras-Chave: |
Acariquara; Analysis; Brasil; Holopyxidium jarana; Jarana; Minquartia punctata; Pau de remo; Pipta dema pereguna; Piquia; Piquiarana; Styrax acuminatum. |
Thesagro: |
Alexa Grandiflora; Análise; Itaúba; Maçaranduba; Madeira; Manilkara Huberi; Melancieira; Mezilaurus Itauba; Papel; Paricá; Polpa; Química; Tecnologia. |
Thesaurus NAL: |
Amazonia; chemistry; paper; pulp; technology; wood. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/57843/1/CPATU-ComTec19.pdf
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Marc: |
LEADER 01387nam a2200505 a 4500 001 1387990 005 2024-08-28 008 1979 bl uuuu 00u1 u #d 100 1 $aMELO, C. F. M. de 245 $aEstudo químico e micrográfico de madeiras da Amazônia. 260 $aBelém, PA: EMBRAPA-CPATU$c1979 300 $a70 p.$cil. 490 $a(EMBRAPA-CPATU. Comunicado técnico, 19). 520 $aIntrodução. Material e métodos. Análises químicas. Estudo micrográfico. Resultados. Características micrográficas das fibras. Discussão e conclusões. Anexos. 650 $aAmazonia 650 $achemistry 650 $apaper 650 $apulp 650 $atechnology 650 $awood 650 $aAlexa Grandiflora 650 $aAnálise 650 $aItaúba 650 $aMaçaranduba 650 $aMadeira 650 $aManilkara Huberi 650 $aMelancieira 650 $aMezilaurus Itauba 650 $aPapel 650 $aParicá 650 $aPolpa 650 $aQuímica 650 $aTecnologia 653 $aAcariquara 653 $aAnalysis 653 $aBrasil 653 $aHolopyxidium jarana 653 $aJarana 653 $aMinquartia punctata 653 $aPau de remo 653 $aPipta dema pereguna 653 $aPiquia 653 $aPiquiarana 653 $aStyrax acuminatum 700 1 $aGOMES, J. I.
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Embrapa Amazônia Oriental (CPATU) |
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