Data/hora: 19/06/2024 22:27:56

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 10/02/2012
Data da última atualização: | 27/02/2023
Autoria: | ABREU, E. F. M.; ANDRADE, E. C. de; ARAGAO, F. J. L.
Afiliação: | Emanuel Felipe Medereiros Abreu, UnB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN.
Título: | Molecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil.
Ano de publicação: | 2011
Fonte/Imprenta: | In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. pdf 34699
Páginas: | p. 6
Idioma: | Inglês
Conteúdo: | Cowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV.
Palavras-Chave: | CABMV; Cowpea; CpSMV; Molecular and RT-PCR analysis; Virus diseases.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)
Exemplares: | CENARGEN - (33773-1) - UPC - PC - DD - SP 20141f -  - SP 20141f - DIS