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Registro Completo |
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
06/02/2018 |
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Data da última atualização: |
06/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, N. L.; CABRAL, G. B.; VIEIRA, P. M.; BALDONI, A. B.; ARAGAO, F. J. L. |
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Afiliação: |
NATÁLIA L. SOUSA, UNB; GLAUCIA BARBOSA CABRAL, Cenargen; PABLINE M. VIEIRA, INSTITUTO FEDERAL GOIANO; AISY BOTEGA BALDONI TARDIN, CPAMT; FRANCISCO JOSE LIMA ARAGAO, Cenargen. |
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Título: |
Bio-detoxification of ricin in castor bean (Ricinus communis L.) seeds. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Scientific Reports, v. .7, n. 1, 2017. (Open). |
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DOI: |
10.1038/s41598-017-15636-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Ricin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society. |
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Palavras-Chave: |
Toxic ribosome-inactivating. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01828naa a2200193 a 4500
001 2087235
005 2018-02-06
008 2017 bl --- 0-- u #d
024 7 $a10.1038/s41598-017-15636-7$2DOI
100 1 $aSOUSA, N. L.
245 $aBio-detoxification of ricin in castor bean (Ricinus communis L.) seeds.$h[electronic resource]
260 $c2017
520 $aRicin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds. Results indicated that ricin genes were effectively silenced in genetically modified (GM) plants, and ricin proteins were not detected by ELISA. Hemagglutination activity was not observed with proteins isolated from GM seeds. In addition, we demonstrated that seed proteins from GM plants were not toxic to rat intestine epithelial cells or to Swiss Webster mice. After oil extraction, bio-detoxified castor bean cake, which is very rich in valuable proteins, can be used for animal feeding. Gene silencing would make castor bean cultivation safer for farmers, industrial workers and society.
653 $aToxic ribosome-inactivating
700 1 $aCABRAL, G. B.
700 1 $aVIEIRA, P. M.
700 1 $aBALDONI, A. B.
700 1 $aARAGAO, F. J. L.
773 $tScientific Reports$gv. .7, n. 1, 2017. (Open).
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Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
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Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
22/12/2020 |
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Data da última atualização: |
22/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, T. R. A.; CARVALHO, H. W. L. de; NASCIMENTO, M.; COSTA, E. F. N.; OLIVEIRA, G. H. F.; GRAVINA, G. A.; AMARAL JUNIOR, A. T.; CARVALHO FILHO, J. L. |
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Afiliação: |
TAMARA REBECCA A. OLIVEIRA; HELIO WILSON LEMOS DE CARVALHO, CPATC; MOYSES NASCIMENTO; EMILIANO FERNANDES NASSAU COSTA, CPATC; GUSTAVO HUGO F. OLIVEIRA; GERALDO A. GRAVINA; ANTONIO T. AMARAL JUNIOR; JOSE LUIZ S. CARVALHO FILHO. |
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Título: |
Adaptability and stability evaluation of maize hybrids using Bayesian segmented regression models. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plos One, v. 30, 2020. |
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DOI: |
https://doi.org/10.1371/journal.pone.0236571 |
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Idioma: |
Inglês |
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Thesagro: |
Citogenética Vegetal; Crescimento; Genética Vegetal; Milho; Resposta da Planta; Variação Genética. |
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Thesaurus NAL: |
Corn; Hybrids. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219585/1/12.-Emiliano-Adaptability-and-stability.pdf
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Marc: |
LEADER 00902naa a2200301 a 4500
001 2128612
005 2020-12-22
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0236571$2DOI
100 1 $aOLIVEIRA, T. R. A.
245 $aAdaptability and stability evaluation of maize hybrids using Bayesian segmented regression models.$h[electronic resource]
260 $c2020
650 $aCorn
650 $aHybrids
650 $aCitogenética Vegetal
650 $aCrescimento
650 $aGenética Vegetal
650 $aMilho
650 $aResposta da Planta
650 $aVariação Genética
700 1 $aCARVALHO, H. W. L. de
700 1 $aNASCIMENTO, M.
700 1 $aCOSTA, E. F. N.
700 1 $aOLIVEIRA, G. H. F.
700 1 $aGRAVINA, G. A.
700 1 $aAMARAL JUNIOR, A. T.
700 1 $aCARVALHO FILHO, J. L.
773 $tPlos One$gv. 30, 2020.
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Registro original: |
Embrapa Tabuleiros Costeiros (CPATC) |
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