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| Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
01/08/1992 |
Data da última atualização: |
08/09/2021 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ARAUJO FILHO, J. A. de. |
Afiliação: |
JOÃO AMBRÓSIO DE ARAÚJO FILHO, CNPC. |
Título: |
Pastoreio múltiplo. |
Ano de publicação: |
1985 |
Fonte/Imprenta: |
In: SIMPÓSIO SOBRE MANEJO DE PASTAGEM, 7., 1984, Piracicaba. Anais... Piracicaba: FEALQ, 1985. p. 209-233. |
Idioma: |
Português |
Conteúdo: |
Objetivos do pastoreio combinado; Diferencas na preferencia alimentar entre herbivoros domesticos e selvagens; Pesquisas em pastoreio multiplo; Pastoreio multiplo- uma opcao para a caatinga nordestina; Variacoes no percentual de cobertura viva; Variacao em disponibilidade de biomassa do estrato herbaceo; Flutuacoes na capacidade de suporte; Efeitos das combinacoes sobre o desenvolvimento dos bovinos; Efeitos das combinacoes sobre o desenvolvimento ponderal dos caprinos; Efeitos das combinacoes sobre o desenvolvimento ponderal dos ovinos; Producao anual de peso vivo animal. |
Palavras-Chave: |
Brasil; Ceará; Grassland Management; Semiárido. |
Thesagro: |
Caprino; Manejo; Ovino; Pastagem; Pastejo. |
Thesaurus Nal: |
Brazil; Goats; Grazing; Pastures; Semiarid zones; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 01322nam a2200289 a 4500 001 1514465 005 2021-09-08 008 1985 bl uuuu u00u1 u #d 100 1 $aARAUJO FILHO, J. A. de 245 $aPastoreio múltiplo.$h[electronic resource] 260 $aIn: SIMPÓSIO SOBRE MANEJO DE PASTAGEM, 7., 1984, Piracicaba. Anais... Piracicaba: FEALQ, 1985. p. 209-233.$c1985 520 $aObjetivos do pastoreio combinado; Diferencas na preferencia alimentar entre herbivoros domesticos e selvagens; Pesquisas em pastoreio multiplo; Pastoreio multiplo- uma opcao para a caatinga nordestina; Variacoes no percentual de cobertura viva; Variacao em disponibilidade de biomassa do estrato herbaceo; Flutuacoes na capacidade de suporte; Efeitos das combinacoes sobre o desenvolvimento dos bovinos; Efeitos das combinacoes sobre o desenvolvimento ponderal dos caprinos; Efeitos das combinacoes sobre o desenvolvimento ponderal dos ovinos; Producao anual de peso vivo animal. 650 $aBrazil 650 $aGoats 650 $aGrazing 650 $aPastures 650 $aSemiarid zones 650 $aSheep 650 $aCaprino 650 $aManejo 650 $aOvino 650 $aPastagem 650 $aPastejo 653 $aBrasil 653 $aCeará 653 $aGrassland Management 653 $aSemiárido
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Embrapa Caprinos e Ovinos (CNPC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
23/03/2020 |
Data da última atualização: |
27/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SILVA, C. de O. G.; TEIXEIRA, S. T.; RODRIGUES, K. B.; SOUZA, A. A.; MONCLARO, A. V.; MENDES, T. D.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; FAVARO, L. C. de L.; ABDELNUR, P. V. |
Afiliação: |
Caio de Oliveira Gorgulho Silva; Tallyta Santos Teixeira, Universidade Federal de Tocantins; Kelly Barreto Rodrigues; Amanda Araujo Souza; Antonielle Vieira Monclaro; THAIS DEMARCHI MENDES, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
Título: |
Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Analytical Methods, n. 2, 2020. |
Idioma: |
Inglês |
Conteúdo: |
Lytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products. MenosLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type... Mostrar Tudo |
Palavras-Chave: |
Caracterização de LPMOs. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02626naa a2200241 a 4500 001 2121326 005 2020-04-27 008 2020 bl uuuu u00u1 u #d 100 1 $aSILVA, C. de O. G. 245 $aCombination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity.$h[electronic resource] 260 $c2020 520 $aLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products. 653 $aCaracterização de LPMOs 700 1 $aTEIXEIRA, S. T. 700 1 $aRODRIGUES, K. B. 700 1 $aSOUZA, A. A. 700 1 $aMONCLARO, A. V. 700 1 $aMENDES, T. D. 700 1 $aRIBEIRO, J. A. de A. 700 1 $aSIQUEIRA, F. G. de 700 1 $aFAVARO, L. C. de L. 700 1 $aABDELNUR, P. V. 773 $tAnalytical Methods$gn. 2, 2020.
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