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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
28/01/2021 |
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Data da última atualização: |
10/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, F. C. B. das; CUNHA, E. F. M.; RODRIGUES, S. de M.; BONFIM, K.; TAVARES, E. J. M.; MOURA, M. F.; SILVA, G. F. da. |
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Afiliação: |
Francisca Chagas Bezerra das Araújo, UFRA; ELISA FERREIRA MOURA CUNHA, CPATU; SIMONE DE MIRANDA RODRIGUES, CPATU; KENNY BONFIM DE ARRUDA CARVALHO, Cenargen; ERALDO JOSE MADUREIRA TAVARES, CPATSA; Mônika Fecury Moura, CPATU; GILVAN FERREIRA DA SILVA, CPAA. |
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Título: |
Identification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Genetic Resources and Crop Evolution, v. 68, p. 1809-1824, 2021. |
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DOI: |
https://doi.org/10.1007/s10722-020-01097-x |
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Idioma: |
Inglês |
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Conteúdo: |
The biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally. MenosThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. T... Mostrar Tudo |
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Palavras-Chave: |
Biofortificação; Raíz rica em carotenoide. |
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Thesagro: |
Carotenóide; Mandioca; Manihot Esculenta; Polimorfismo; Proteína; Raiz Alimentícia; Variação Genética. |
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Thesaurus Nal: |
Carotenoids; Cassava; Haplotypes; Polymorphism. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
LEADER 02740naa a2200361 a 4500
001 2129648
005 2021-06-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10722-020-01097-x$2DOI
100 1 $aARAÚJO, F. C. B. das
245 $aIdentification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon.$h[electronic resource]
260 $c2021
520 $aThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally.
650 $aCarotenoids
650 $aCassava
650 $aHaplotypes
650 $aPolymorphism
650 $aCarotenóide
650 $aMandioca
650 $aManihot Esculenta
650 $aPolimorfismo
650 $aProteína
650 $aRaiz Alimentícia
650 $aVariação Genética
653 $aBiofortificação
653 $aRaíz rica em carotenoide
700 1 $aCUNHA, E. F. M.
700 1 $aRODRIGUES, S. de M.
700 1 $aBONFIM, K.
700 1 $aTAVARES, E. J. M.
700 1 $aMOURA, M. F.
700 1 $aSILVA, G. F. da
773 $tGenetic Resources and Crop Evolution$gv. 68, p. 1809-1824, 2021.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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| Registros recuperados : 13 | |
| 2. |  | MAGNABOSCO, C. de U.; MANICARDI, F.; BARBOSA, V.; ARAUJO, F. C. R.; SAINZ, R. D. Analise de caracteristicas de carcaca e crescimento em bovinos da raca Brahman criados na regiao do Mato Grosso- resultados preliminares. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 40., 2003, Santa Maria, RS. Otimizando a producao animal: anais. Santa Maria: SBZ: UFSM, 2003. 1 CD-ROM.| Biblioteca(s): Embrapa Cerrados. |
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| 3. | | PEREIRA, F. D. de M.; OLIVEIRA, B. S. de; ARAÚJO, F. C.; LIMA, P. S. da C. Análise de similaridade de acessos de babaçu por meio de marcadores ISSR. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 8., 2022, Teresina, PI. Anais... Teresina: Embrapa Meio-Norte, 2023. p. 18. (Embrapa Meio-Norte. Documentos, 291).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 6. | | PARIZ, C. M.; AZENHA, M. V.; ANDREOTTI, M.; ARAÚJO, F. C. de M.; ULIAN, N. de A.; BERGAMASCHINE, A. F. Produção e composição bromatológica de forrageiras em sistema de integração lavoura-pecuária em diferentes épocas de semeadura. Pesquisa agropecuária Brasileira, Brasília, DF, v. 46, n. 10, p. 1392-1400, out. 2011 Título em inglês: Yield and chemical composition of forage in crop?livestock integration in different sowing time.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | ARAUJO, F. C. de; NASCENTE, A. S.; FILIPPI, M. C. C. de; SILVA, M. A.; SOUSA, V. S.; LANNA, A. C. Cover crops and multifunctional microorganisms can affect development of upland rice. Australian Journal of Crop Science, v. 15, n. 1, p. 137-144, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 8. | | ARAÚJO, F. C. de; NASCENTE, A. S.; GUIMARÃES, J. L. N.; SOUSA, V. S.; FREITAS, M. A. M. de; SANTOS, F. L. de S. Cover crops in the off-season in the weed management at no-tillage area. Revista Caatinga, v. 34, n. 1, p. 50-57, jan./mar. 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 9. | | ARAÚJO, F. C. B. das; CUNHA, E. F. M.; RODRIGUES, S. de M.; BONFIM, K.; TAVARES, E. J. M.; MOURA, M. F.; SILVA, G. F. da. Identification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon. Genetic Resources and Crop Evolution, v. 68, p. 1809-1824, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 10. | | NASCENTE, A. S.; ARAÚJO, F. C. de; FILIPPI, M. C. C. de; LANNA, A. C.; SILVA, M. A.; SOUZA, V. S. Plantas de cobertura e microrganismos benéficos afetando o desenvolvimento do arroz de terras altas. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 11., 2019. Balneário Camboriú, SC. Inovação e desenvolvimento na orizicultura: anais eletrônico. Itajaí: Epagri: Sosbai, 2019.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 11. | | VASCONCELOS, A. A.; DANTAS FILHO, H. A.; DANTAS, K. G. F.; GOMES, B. S.; CUNHA, R. L.; CUNHA, E. F. M.; ARAÚJO, F. C. B.; LOPES, A. S.; CAMPOS, W. E. O.; TAUBE, P. S. Determinação dos teores de Cu, Fe, Mn e Zn por FAAS em raízes de mandioca (Manihot esculenta Crantz) da Amazônia Oriental. Revista Virtual de Química, v. 9, n. 6, p. 2316-2331, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | RONCHI, C. P.; ARAÚJO, F. C. de; ALMEIDA, W. L. de; SILVA, M. A. A. da; MAGALHÃES, C. E. de O.; OLIVEIRA, L. B. de; DRUMOND, L. C. D. Respostas ecofisiológicas de cafeeiros submetidos ao deficit hídrico para concentração da florada no Cerrado de Minas Gerais. PESQUISA AGROPECUÁRIA BRASILEIRA, Brasília, DF, v. 50, n. 1, p.24-32, jan., 2015. Título em inglês: Ecophysiological responses of coffee plants subjected to water deficit to narrow blossom period in the Cerrado in the state of Minas Gerais, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 13. | | FERREIRA-NETO, R, C.; ARAÚJO, F. C. de; SILVA, R. L. de O.; MELO, N. F. de; PANDOLF, V.; FROSI, G.; MORAIS, D. A. de L.; SILVA, M. D. da; RIVAS, R.; SANTOS, M. G.; AIDAR, S. de T.; MORGANTE, C. V.; BENKO-ISEPPON, A. M. Dehydration response in Stylosanthesscabra: transcriptional, biochemical, and physiological modulations. Physiologia Plantarum, 174, e13821. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 13 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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