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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
27/09/2012 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, E. R.; COSTA, J. R.; FERREIRA, M. A. S. V.; QUEZADO-DUVAL, A. M. |
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Afiliação: |
ALICE MARIA QUEZADO DUVAL, CNPH. |
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Título: |
Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Applied Microbiology, Oxford, Sept. 2012. |
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ISSN: |
1364-5072 |
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DOI: |
10.1111/j.1365-2672.05431.x |
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Idioma: |
Inglês |
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Conteúdo: |
To establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification. |
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Palavras-Chave: |
Detecção; PCR. |
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Thesagro: |
Bactéria; Doença; Identificação. |
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Thesaurus Nal: |
Xanthomonas. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177608/1/Ara-jo-et-al-2012-Journal-of-Applied-Microbiology.pdf
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Marc: |
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022 $a1364-5072
024 7 $a10.1111/j.1365-2672.05431.x$2DOI
100 1 $aARAÚJO, E. R.
245 $aSimultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.$h[electronic resource]
260 $c2012
520 $aTo establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification.
650 $aXanthomonas
650 $aBactéria
650 $aDoença
650 $aIdentificação
653 $aDetecção
653 $aPCR
700 1 $aCOSTA, J. R.
700 1 $aFERREIRA, M. A. S. V.
700 1 $aQUEZADO-DUVAL, A. M.
773 $tJournal of Applied Microbiology, Oxford, Sept. 2012.
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Registro original: |
Embrapa Hortaliças (CNPH) |
