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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
20/12/2004 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOMES, E. A.; KASUYA, M. C. M.; BARROS, E. G. de; BORGES, A. C.; ARAUJO, E. F. |
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Afiliação: |
ELIANE APARECIDA GOMES, CNPMS. |
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Título: |
Polymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 25, n. 4, p. 477-483, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Inter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera and most species presented specific restriction patterns. Species not identifiable by a specific pattern belonged to two genera: Rhizopogon (R. nigrescens, R. reaii, R. roseolus, R. rubescens and Rhizopogon sp.), and Laccaria (L. bicolor and L. amethystea). Our data confirm the potential of ITS region PCR-RFLP for the molecular characterization of ectomycorrhizal fungi and their identification and monitoring in artificial inoculation programs. |
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Palavras-Chave: |
Ectomycorrhizal fungi; Internal transcribed spacer (ITS); PCR-RFLP; Ribosomal DNA (rDNA). |
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Thesaurus Nal: |
DNA fingerprinting. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32594/1/Polymorphism-internal.pdf
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Marc: |
LEADER 01964naa a2200229 a 4500
001 1488489
005 2018-05-30
008 2002 bl uuuu u00u1 u #d
100 1 $aGOMES, E. A.
245 $aPolymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi.$h[electronic resource]
260 $c2002
520 $aInter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera and most species presented specific restriction patterns. Species not identifiable by a specific pattern belonged to two genera: Rhizopogon (R. nigrescens, R. reaii, R. roseolus, R. rubescens and Rhizopogon sp.), and Laccaria (L. bicolor and L. amethystea). Our data confirm the potential of ITS region PCR-RFLP for the molecular characterization of ectomycorrhizal fungi and their identification and monitoring in artificial inoculation programs.
650 $aDNA fingerprinting
653 $aEctomycorrhizal fungi
653 $aInternal transcribed spacer (ITS)
653 $aPCR-RFLP
653 $aRibosomal DNA (rDNA)
700 1 $aKASUYA, M. C. M.
700 1 $aBARROS, E. G. de
700 1 $aBORGES, A. C.
700 1 $aARAUJO, E. F.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 25, n. 4, p. 477-483, 2002.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/07/2021 |
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Data da última atualização: |
19/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERREIRA, J. A.; QUEIROZ, S. C. do N. de. |
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Afiliação: |
JORDANA ALVES FERREIRA; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA. |
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Título: |
Multiresidue method for determination of pesticides in coconut (Cocos nucifera Linn.) endosperm by using GC-MS/MS and UHPLC-MS/MS analysis. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Journal of Food Composition and Analysis, v. 97, article 103764, 2021. |
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ISSN: |
0889-1575 |
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DOI: |
https://doi.org/10.1016/j.jfca.2020.103764 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Several pesticides are used in the cultivation of coconut palm, hence evaluation of the residues from these substances must be analyzed in the fruit, to provide and ensure the food safety. Therefore, the development and validation of comprehensive and effective methods becomes crucial, given the complexity of the matrix. Thus, a novel method was developed for the determination of 10 different pesticides residues in coconut endosperm. QuEChERS showed to be an efficient method for sample preparation to overcome the complexity of the matrix. To extend the analytical scope, including a wide range of polarities, ultrahigh-performance liquid chromatography coupled mass spectrometry (UHPLC-MS/MS) and gas chromatography coupled mass spectrometry (GC--MS/MS) were employed. Triple quadrupole spectrometer was used in selected reaction monitoring (SRM) on both detectors, providing high sensitivity and selectivity. These approaches showed to be optimal, considering the parameters of linearity (r >= 0.99), matrix effect (0-50 %), LOQ (5 microgram kg-1, except for thiamethoxam (20 microgram kg-1)), specificity, trueness (60-118 %) and precision (repeatability and within-laboratory reproducibility) <= 20 %. The concentration levels obtained were higher than the MRL recommended for thiamethoxam (EU regulation <10 microgram g kg-1), justifying the analysis of such residues in commercial coconut endosperm. |
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Thesagro: |
Agrotóxico; Água de Coco; Análise Química; Coco. |
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Thesaurus NAL: |
Coconut water; Detection; Gas chromatography-mass spectrometry; Pesticide residues; Ultra-performance liquid chromatography. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02327naa a2200265 a 4500
001 2133021
005 2021-07-19
008 2021 bl uuuu u00u1 u #d
022 $a0889-1575
024 7 $ahttps://doi.org/10.1016/j.jfca.2020.103764$2DOI
100 1 $aFERREIRA, J. A.
245 $aMultiresidue method for determination of pesticides in coconut (Cocos nucifera Linn.) endosperm by using GC-MS/MS and UHPLC-MS/MS analysis.$h[electronic resource]
260 $c2021
520 $aAbstract: Several pesticides are used in the cultivation of coconut palm, hence evaluation of the residues from these substances must be analyzed in the fruit, to provide and ensure the food safety. Therefore, the development and validation of comprehensive and effective methods becomes crucial, given the complexity of the matrix. Thus, a novel method was developed for the determination of 10 different pesticides residues in coconut endosperm. QuEChERS showed to be an efficient method for sample preparation to overcome the complexity of the matrix. To extend the analytical scope, including a wide range of polarities, ultrahigh-performance liquid chromatography coupled mass spectrometry (UHPLC-MS/MS) and gas chromatography coupled mass spectrometry (GC--MS/MS) were employed. Triple quadrupole spectrometer was used in selected reaction monitoring (SRM) on both detectors, providing high sensitivity and selectivity. These approaches showed to be optimal, considering the parameters of linearity (r >= 0.99), matrix effect (0-50 %), LOQ (5 microgram kg-1, except for thiamethoxam (20 microgram kg-1)), specificity, trueness (60-118 %) and precision (repeatability and within-laboratory reproducibility) <= 20 %. The concentration levels obtained were higher than the MRL recommended for thiamethoxam (EU regulation <10 microgram g kg-1), justifying the analysis of such residues in commercial coconut endosperm.
650 $aCoconut water
650 $aDetection
650 $aGas chromatography-mass spectrometry
650 $aPesticide residues
650 $aUltra-performance liquid chromatography
650 $aAgrotóxico
650 $aÁgua de Coco
650 $aAnálise Química
650 $aCoco
700 1 $aQUEIROZ, S. C. do N. de
773 $tJournal of Food Composition and Analysis$gv. 97, article 103764, 2021.
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Embrapa Meio Ambiente (CNPMA) |
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