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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
24/02/2015 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRAGA, R. M.; SANTANA, M. F.; COSTA, R. V. da; BROMMONSCHENKEL, S. H.; ARAÚJO, E. F. de; QUEIROZ, M. V. de. |
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Afiliação: |
RODRIGO VERAS DA COSTA, CNPMS. |
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Título: |
Transposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Mycologia, New York, v. 106, n. 4, p. 629-641, 2014. |
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DOI: |
10.3852/13?262 |
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Idioma: |
Inglês |
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Conteúdo: |
Transposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon MenosTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the char... Mostrar Tudo |
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Thesagro: |
Antracnose. |
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Categoria do assunto: |
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Marc: |
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008 2014 bl uuuu u00u1 u #d
024 7 $a10.3852/13?262$2DOI
100 1 $aBRAGA, R. M.
245 $aTransposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola.$h[electronic resource]
260 $c2014
520 $aTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon
650 $aAntracnose
700 1 $aSANTANA, M. F.
700 1 $aCOSTA, R. V. da
700 1 $aBROMMONSCHENKEL, S. H.
700 1 $aARAÚJO, E. F. de
700 1 $aQUEIROZ, M. V. de
773 $tMycologia, New York$gv. 106, n. 4, p. 629-641, 2014.
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Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 75 | |
| 6. |  | ARAUJO, E. F.; ZONTA, J. B.; ARAUJO, R. F.; DIAS, L. A. dos S. Armazenamento de sementes de pinhão manso em diferentes embalagens e ambientes. In: CONGRESSO BRASILEIRO DE SEMENTES, 17., 2011, Natal, RN. A semente no contexto da inovação e sustentabilidade. Londrina, PR: Associação Brasileira de Tecnologia de Sementes, 2011. 1 p. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cocais. |
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| 9. | | ARAUJO, R. F.; ZONTA, J. B.; ARAUJO, E. F.; DIAS, L. A. dos S. Efeito da massa específica e do tamanho na qualidade fisiológica de sementes de pinhão manso. In: CONGRESSO BRASILEIRO DE SEMENTES, 17., 2011, Natal, RN. A semente no contexto da inovação e sustentabilidade. Londrina, PR: Associação Brasileira de Tecnologia de Sementes, 2011. 1 p. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cocais. |
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| 10. | | ZONTA, J. B.; ARAÚJO, E. F.; ARAÚJO, R. F.; ZUCHI, J.; ZONTA, J. H. Efeito da massa específica e do tamanho na qualidade fisiológica de sementes de pinhão manso. Revista Brasileira de Oleaginosas e Fibrosas, Campina Grande, PB, v.15, n.1, p. 17-26, jan./abr.2011. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Cocais. |
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| 11. | | ARAUJO, E. F.; ZONTA, J. B.; ARAUJO, R. F.; DIAS, L. A. dos S. Efeito da secagem na conservação de sementes de pinhão manso. In: CONGRESSO BRASILEIRO DE SEMENTES, 17., 2011, Natal, RN. A semente no contexto da inovação e sustentabilidade. Londrina, PR: Associação Brasileira de Tecnologia de Sementes, 2011. 1 p. Resumos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cocais. |
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| 13. | | ARAUJO, R. F.; DONZELLES, S. M. L.; ZONTA, J. B.; ARAUJO, E. F. Conservação de sementes de feijão tratadas com cera de carnaúba. In: CONGRESSO BRASILEIRO DE SEMENTES, 17., 2011, Natal, RN. A semente no contexto da inovação e sustentabilidade. Londrina, PR: Associação Brasileira de Tecnologia de Sementes, 2011. 1 p. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cocais. |
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| 14. | | ZONTA, J. B.; ARAÚJO, E. F.; ARAÚJO, R. F.; ZONTA, F. M. G.; REIS, M. S. Characterization of mechanical damage in coffee seeds by the LERCAFÉ test. IDESIA, Chile, v.29, n.3, p. 33-38, set./dez. 2011. 6 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Cocais. |
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| 15. | | VALENTE, M. L.; FERRAZ, M. de O.; SCHUMACHER, M. V.; TRUEBY, P.; ARAÚJO, E. F. Interceptação da água da chuva em um povoamento de eucalipto no município de Eldorado do Sul-RS. In: CONGRESSO BRASILEIRO SOBRE FLORESTAS ENERGÉTICAS, 1., 2009, Belo Horizonte. Anais. Colombo: Embrapa Florestas, 2009. 1 CD-ROM. (Embrapa Florestas. Documentos, 178). Seção: Sustentabilidade Ambiental, Social e Econômica.| Biblioteca(s): Embrapa Florestas. |
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| 17. | | ZONTA, J. B.; ARAUJO, E. F.; ARAUJO, R. F.; REIS, M. S. Uso do teste Lercafé para a caracterização de danos em sementes de cafeeiro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 11, p. 1601-1607, nov. 2008. Título em inglês: Characterization of injuries in coffee seeds through Lercafé test.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 19. | | ABUD, H. F.; ARAUJO, E. F.; ARAUJO, R. F.; ARAUJO, A. V.; PINTO, C. M. F. Qualidade fisiológica de sementes das pimentas malagueta e biquinho durante a ontogênese. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 12, p. 1546-1554, dez. 2013. Título em inglês: Physiological quality of "malagueta" and "biquinho" pepper seeds during ontogeny.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 20. | | NUNES, J. C. S.; FONTES, P. C. R.; ARAÚJO, E. F.; SEDIYAMA, C. Potato plant growth and macronutrient uptake as affected by soil tillage and irrigation systems. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 12, p. 1787-1792, dez. 2006 Título em portguês: Crescimento da batateira e absorção de macronutrientes influenciados pelos sistemas de preparo de solo e de irrigação.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 75 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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