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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados; Embrapa Soja. |
Data corrente: |
27/05/1992 |
Data da última atualização: |
03/11/1994 |
Autoria: |
ANJOS, J. R. N. dos. |
Título: |
Studies on the epidemiology and molecular biology of two soybean viruses: soybean mosaic virus and bean pod mottle virus. |
Ano de publicação: |
1991 |
Fonte/Imprenta: |
Lexington, Kentucky: University of Kentucky, 1991. |
Páginas: |
162p. |
Idioma: |
Inglês |
Notas: |
Tese Doutorado. |
Conteúdo: |
Soybean (Glycine max (L.) Merr.) is a major crop in Kentuchy. Soybean mosaic virus (SMV), bean pod mottle virus (BPMV), and dual infection with SMV and BPMV present a significant potential threat to soybean production in the state. The specific goals of this study were to identify the primary sources of BPMV inoculum in soybean fields; to investigate the mechanisms underlying the synergistic interactions between SMV and BPMV; and to construct chimeric genes containg SMV coat protein (CP) sequence for the purpose of producing CP-mediated transgenic resistance to SMV in soybean. The significance of overwintering bean leaf beetles, vectors of BPMV, and seeds from infected soybean plants as sources of primary virus inoculum was assessed. Although BPMV antigen wasreadily detected by ELISA in regurgitant from naturally overwintered beatles, none of these beetles transmitted the virus to healthy soybean seedlings. An average of 6.6% of transmission, however, was observed when beetles were exposed to artificial overwintering conditions. No apparent structural changes in BPMV RNA could be related to the loss or decline of BPMV infectivity in overwintering beetles. On the other hand, evidence is presented to indicate that exposure of BPMV to beetle regurgitant led to proteolysis of the CP. The proteolysis was not limited to the small 22 kDa subunit, as would be expected from the generation of heterologous electrophoretic forms, but extended to the large 41 kDa subunit. The cleavage site in the 41 kDa polypeptide was ... MenosSoybean (Glycine max (L.) Merr.) is a major crop in Kentuchy. Soybean mosaic virus (SMV), bean pod mottle virus (BPMV), and dual infection with SMV and BPMV present a significant potential threat to soybean production in the state. The specific goals of this study were to identify the primary sources of BPMV inoculum in soybean fields; to investigate the mechanisms underlying the synergistic interactions between SMV and BPMV; and to construct chimeric genes containg SMV coat protein (CP) sequence for the purpose of producing CP-mediated transgenic resistance to SMV in soybean. The significance of overwintering bean leaf beetles, vectors of BPMV, and seeds from infected soybean plants as sources of primary virus inoculum was assessed. Although BPMV antigen wasreadily detected by ELISA in regurgitant from naturally overwintered beatles, none of these beetles transmitted the virus to healthy soybean seedlings. An average of 6.6% of transmission, however, was observed when beetles were exposed to artificial overwintering conditions. No apparent structural changes in BPMV RNA could be related to the loss or decline of BPMV infectivity in overwintering beetles. On the other hand, evidence is presented to indicate that exposure of BPMV to beetle regurgitant led to proteolysis of the CP. The proteolysis was not limited to the small 22 kDa subunit, as would be expected from the generation of heterologous electrophoretic forms, but extended to the large 41 kDa subunit. The cleavage si... Mostrar Tudo |
Palavras-Chave: |
Disease; Insetos vetores; Mosaic; SMV; Soybean; Virus do Mosaico; Virus do Mosqueado. |
Thesagro: |
Doença; Feijão; Glycine Max; Mosaico; Phaseolus Vulgaris; Soja; Vagem; Vírus. |
Thesaurus Nal: |
insects; viruses. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02408nam a2200337 a 4500 001 1555610 005 1994-11-03 008 1991 bl uuuu m 00u1 u #d 100 1 $aANJOS, J. R. N. dos 245 $aStudies on the epidemiology and molecular biology of two soybean viruses$bsoybean mosaic virus and bean pod mottle virus. 260 $aLexington, Kentucky: University of Kentucky$c1991 300 $a162p. 500 $aTese Doutorado. 520 $aSoybean (Glycine max (L.) Merr.) is a major crop in Kentuchy. Soybean mosaic virus (SMV), bean pod mottle virus (BPMV), and dual infection with SMV and BPMV present a significant potential threat to soybean production in the state. The specific goals of this study were to identify the primary sources of BPMV inoculum in soybean fields; to investigate the mechanisms underlying the synergistic interactions between SMV and BPMV; and to construct chimeric genes containg SMV coat protein (CP) sequence for the purpose of producing CP-mediated transgenic resistance to SMV in soybean. The significance of overwintering bean leaf beetles, vectors of BPMV, and seeds from infected soybean plants as sources of primary virus inoculum was assessed. Although BPMV antigen wasreadily detected by ELISA in regurgitant from naturally overwintered beatles, none of these beetles transmitted the virus to healthy soybean seedlings. An average of 6.6% of transmission, however, was observed when beetles were exposed to artificial overwintering conditions. No apparent structural changes in BPMV RNA could be related to the loss or decline of BPMV infectivity in overwintering beetles. On the other hand, evidence is presented to indicate that exposure of BPMV to beetle regurgitant led to proteolysis of the CP. The proteolysis was not limited to the small 22 kDa subunit, as would be expected from the generation of heterologous electrophoretic forms, but extended to the large 41 kDa subunit. The cleavage site in the 41 kDa polypeptide was ... 650 $ainsects 650 $aviruses 650 $aDoença 650 $aFeijão 650 $aGlycine Max 650 $aMosaico 650 $aPhaseolus Vulgaris 650 $aSoja 650 $aVagem 650 $aVírus 653 $aDisease 653 $aInsetos vetores 653 $aMosaic 653 $aSMV 653 $aSoybean 653 $aVirus do Mosaico 653 $aVirus do Mosqueado
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
04/06/2020 |
Data da última atualização: |
15/12/2020 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SANTOS, I. S.; SAMPAIO, S. R.; LIMA, L. K. S.; SOARES, T. L.; JESUS, O. N. de. |
Afiliação: |
IDÁLIA SOUZA SANTOS, UFRB; SIDNARA RIBEIRO SAMPAIO, UFRB; LUCAS KENNEDY SILVA LIMA, NPq; TALIANE LEILA SOARES, CAPES; ONILDO NUNES DE JESUS, CNPMF. |
Título: |
Avaliação agronômica de progênies interespecíficas de maracujazeiro oriundas de ciclos de retrocruzamento. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 13., 2019. Foco e valor : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura,2019. 119 p. il. |
Idioma: |
Português |
Thesagro: |
Maracujá. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213680/1/ANAIS-2019-Ainfo-33.pdf
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Marc: |
LEADER 00636nam a2200157 a 4500 001 2122994 005 2020-12-15 008 2019 bl uuuu u00u1 u #d 100 1 $aSANTOS, I. S. 245 $aAvaliação agronômica de progênies interespecíficas de maracujazeiro oriundas de ciclos de retrocruzamento.$h[electronic resource] 260 $aIn: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 13., 2019. Foco e valor : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura,2019. 119 p. il.$c2019 650 $aMaracujá 700 1 $aSAMPAIO, S. R. 700 1 $aLIMA, L. K. S. 700 1 $aSOARES, T. L. 700 1 $aJESUS, O. N. de
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Embrapa Mandioca e Fruticultura (CNPMF) |
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