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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Soja. |
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Data corrente: |
27/05/1992 |
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Data da última atualização: |
03/11/1994 |
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Autoria: |
ANJOS, J. R. N. dos. |
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Título: |
Studies on the epidemiology and molecular biology of two soybean viruses: soybean mosaic virus and bean pod mottle virus. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Lexington, Kentucky: University of Kentucky, 1991. |
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Páginas: |
162p. |
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Idioma: |
Inglês |
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Notas: |
Tese Doutorado. |
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Conteúdo: |
Soybean (Glycine max (L.) Merr.) is a major crop in Kentuchy. Soybean mosaic virus (SMV), bean pod mottle virus (BPMV), and dual infection with SMV and BPMV present a significant potential threat to soybean production in the state. The specific goals of this study were to identify the primary sources of BPMV inoculum in soybean fields; to investigate the mechanisms underlying the synergistic interactions between SMV and BPMV; and to construct chimeric genes containg SMV coat protein (CP) sequence for the purpose of producing CP-mediated transgenic resistance to SMV in soybean. The significance of overwintering bean leaf beetles, vectors of BPMV, and seeds from infected soybean plants as sources of primary virus inoculum was assessed. Although BPMV antigen wasreadily detected by ELISA in regurgitant from naturally overwintered beatles, none of these beetles transmitted the virus to healthy soybean seedlings. An average of 6.6% of transmission, however, was observed when beetles were exposed to artificial overwintering conditions. No apparent structural changes in BPMV RNA could be related to the loss or decline of BPMV infectivity in overwintering beetles. On the other hand, evidence is presented to indicate that exposure of BPMV to beetle regurgitant led to proteolysis of the CP. The proteolysis was not limited to the small 22 kDa subunit, as would be expected from the generation of heterologous electrophoretic forms, but extended to the large 41 kDa subunit. The cleavage site in the 41 kDa polypeptide was ... MenosSoybean (Glycine max (L.) Merr.) is a major crop in Kentuchy. Soybean mosaic virus (SMV), bean pod mottle virus (BPMV), and dual infection with SMV and BPMV present a significant potential threat to soybean production in the state. The specific goals of this study were to identify the primary sources of BPMV inoculum in soybean fields; to investigate the mechanisms underlying the synergistic interactions between SMV and BPMV; and to construct chimeric genes containg SMV coat protein (CP) sequence for the purpose of producing CP-mediated transgenic resistance to SMV in soybean. The significance of overwintering bean leaf beetles, vectors of BPMV, and seeds from infected soybean plants as sources of primary virus inoculum was assessed. Although BPMV antigen wasreadily detected by ELISA in regurgitant from naturally overwintered beatles, none of these beetles transmitted the virus to healthy soybean seedlings. An average of 6.6% of transmission, however, was observed when beetles were exposed to artificial overwintering conditions. No apparent structural changes in BPMV RNA could be related to the loss or decline of BPMV infectivity in overwintering beetles. On the other hand, evidence is presented to indicate that exposure of BPMV to beetle regurgitant led to proteolysis of the CP. The proteolysis was not limited to the small 22 kDa subunit, as would be expected from the generation of heterologous electrophoretic forms, but extended to the large 41 kDa subunit. The cleavage si... Mostrar Tudo |
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Palavras-Chave: |
Disease; Insetos vetores; Mosaic; SMV; Soybean; Virus do Mosaico; Virus do Mosqueado. |
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Thesagro: |
Doença; Feijão; Glycine Max; Mosaico; Phaseolus Vulgaris; Soja; Vagem; Vírus. |
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Thesaurus Nal: |
insects; viruses. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02408nam a2200337 a 4500
001 1555610
005 1994-11-03
008 1991 bl uuuu m 00u1 u #d
100 1 $aANJOS, J. R. N. dos
245 $aStudies on the epidemiology and molecular biology of two soybean viruses$bsoybean mosaic virus and bean pod mottle virus.
260 $aLexington, Kentucky: University of Kentucky$c1991
300 $a162p.
500 $aTese Doutorado.
520 $aSoybean (Glycine max (L.) Merr.) is a major crop in Kentuchy. Soybean mosaic virus (SMV), bean pod mottle virus (BPMV), and dual infection with SMV and BPMV present a significant potential threat to soybean production in the state. The specific goals of this study were to identify the primary sources of BPMV inoculum in soybean fields; to investigate the mechanisms underlying the synergistic interactions between SMV and BPMV; and to construct chimeric genes containg SMV coat protein (CP) sequence for the purpose of producing CP-mediated transgenic resistance to SMV in soybean. The significance of overwintering bean leaf beetles, vectors of BPMV, and seeds from infected soybean plants as sources of primary virus inoculum was assessed. Although BPMV antigen wasreadily detected by ELISA in regurgitant from naturally overwintered beatles, none of these beetles transmitted the virus to healthy soybean seedlings. An average of 6.6% of transmission, however, was observed when beetles were exposed to artificial overwintering conditions. No apparent structural changes in BPMV RNA could be related to the loss or decline of BPMV infectivity in overwintering beetles. On the other hand, evidence is presented to indicate that exposure of BPMV to beetle regurgitant led to proteolysis of the CP. The proteolysis was not limited to the small 22 kDa subunit, as would be expected from the generation of heterologous electrophoretic forms, but extended to the large 41 kDa subunit. The cleavage site in the 41 kDa polypeptide was ...
650 $ainsects
650 $aviruses
650 $aDoença
650 $aFeijão
650 $aGlycine Max
650 $aMosaico
650 $aPhaseolus Vulgaris
650 $aSoja
650 $aVagem
650 $aVírus
653 $aDisease
653 $aInsetos vetores
653 $aMosaic
653 $aSMV
653 $aSoybean
653 $aVirus do Mosaico
653 $aVirus do Mosqueado
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Registros recuperados : 71 | |
| 19. |  | VIEIRA, E. A.; CHARCHAR, M. J. d'A.; SILVA, M. S.; ANJOS, J. R. N. dos. Virulência de isolados de Colletotrichum gloeosporioides de populações selvagens de Stylosanthes spp. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 5, p. 661-667, maio 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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| Registros recuperados : 71 | |
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