Data/hora: 24/06/2024 06:04:48

Biblioteca(s): | Embrapa Cerrados; Embrapa Soja.
Data corrente: | 27/05/1992
Data da última atualização: | 03/11/1994
Autoria: | ANJOS, J. R. N. dos.
Título: | Studies on the epidemiology and molecular biology of two soybean viruses: soybean mosaic virus and bean pod mottle virus.
Ano de publicação: | 1991
Fonte/Imprenta: | Lexington, Kentucky: University of Kentucky, 1991.
Páginas: | 162p.
Idioma: | Inglês
Notas: | Tese Doutorado.
Conteúdo: | Soybean (Glycine max (L.) Merr.) is a major crop in Kentuchy. Soybean mosaic virus (SMV), bean pod mottle virus (BPMV), and dual infection with SMV and BPMV present a significant potential threat to soybean production in the state. The specific goals of this study were to identify the primary sources of BPMV inoculum in soybean fields; to investigate the mechanisms underlying the synergistic interactions between SMV and BPMV; and to construct chimeric genes containg SMV coat protein (CP) sequence for the purpose of producing CP-mediated transgenic resistance to SMV in soybean. The significance of overwintering bean leaf beetles, vectors of BPMV, and seeds from infected soybean plants as sources of primary virus inoculum was assessed. Although BPMV antigen wasreadily detected by ELISA in regurgitant from naturally overwintered beatles, none of these beetles transmitted the virus to healthy soybean seedlings. An average of 6.6% of transmission, however, was observed when beetles were exposed to artificial overwintering conditions. No apparent structural changes in BPMV RNA could be related to the loss or decline of BPMV infectivity in overwintering beetles. On the other hand, evidence is presented to indicate that exposure of BPMV to beetle regurgitant led to proteolysis of the CP. The proteolysis was not limited to the small 22 kDa subunit, as would be expected from the generation of heterologous electrophoretic forms, but extended to the large 41 kDa subunit. The cleavage site in the 41 kDa polypeptide was ...
Palavras-Chave: | Disease; Insetos vetores; Mosaic; SMV; Soybean; Virus do Mosaico; Virus do Mosqueado.
Thesagro: | Doença; Feijão; Glycine Max; Mosaico; Phaseolus Vulgaris; Soja; Vagem; Vírus.
Thesaurus Nal: | insects; viruses.
Categoria do assunto: | --
Registro original: | Embrapa Cerrados (CPAC)

Exemplares:

CNPSO - (15059-1) - ADD - TS - -- - 7/91 -  - 7/91 - DIS
CPAC - (12430-1) - ADD - TS - -- - T007/91 -  - T007/91 - DIS