Data/hora: 16/06/2024 11:10:29

Biblioteca(s): | Embrapa Café.
Data corrente: | 12/09/2022
Data da última atualização: | 12/04/2023
Autoria: | ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T.
Afiliação: | PAULA CRISTINA DA SILVA ANGELO, CNPCa.
Título: | Coffee microspore cultivation to attain doubled-haploid stable plantlets.
Ano de publicação: | 2022
Fonte/Imprenta: | INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.
DOI: | 10.17660/ActaHortic.2023.1359.14
Idioma: | Inglês
Notas: | E-poster.
Conteúdo: | The aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli produced by the interspecific hybrid anthers. Regarding the hybrid anthers, 25% were infected by a same endogenous bacteria but 8% produced embryogenic tissue, with globular embryos multiplying and maturing simultaneously upon transfer to N6 medium without growth regulators. After 1.2 years, the photomorphogenesis has been induced under light, and auxin/cytokinin ratio = 2 plus GA3.
Thesagro: | Café; Embriogénese; Pólen; Rubiaceae.
Thesaurus Nal: | Embryogenesis; Pollen.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/249059/1/Coffee-microspore-cultivation.pdf
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (1617-1) - UPC - RA - DD -  -  -  - DIS