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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/03/2011 |
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Data da última atualização: |
09/09/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ANDREOTTI, R.; SOARES, M.; CUNHA, R. C.; LEITE, F. P. L. |
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Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; UFPEL; UFPEL; UFPEL. |
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Título: |
Heterologous expression in Pichia pastoris of gene BMTI optimized. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010. |
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Páginas: |
p.149-152 |
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Idioma: |
Inglês
Português |
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Notas: |
ICOPA XII |
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Conteúdo: |
The cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was
expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo. MenosThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same... Mostrar Tudo |
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Thesagro: |
Carrapato; Sanidade Animal. |
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Thesaurus Nal: |
Rhipicephalus microplus. |
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Categoria do assunto: |
H Saúde e Patologia
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02512naa a2200217 a 4500
001 1883447
005 2014-09-09
008 2010 bl --- 0-- u #d
100 1 $aANDREOTTI, R.
245 $aHeterologous expression in Pichia pastoris of gene BMTI optimized.
260 $c2010
300 $ap.149-152
500 $aICOPA XII
520 $aThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo.
650 $aRhipicephalus microplus
650 $aCarrapato
650 $aSanidade Animal
700 1 $aSOARES, M.
700 1 $aCUNHA, R. C.
700 1 $aLEITE, F. P. L.
773 $tIn: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Registros recuperados : 301 | |
| 167. |  | MIZIARA, S. R.; PAIVA, F.; ANDREOTTI, R.; KOLLER, W. W.; LOPES, V. Ocorrência de Ixodes ioricatus (Neumann, 1899) (Acari: Ixodidae) parasitando Didelphis albiventris (Lund, 1841), (Didelphimorphia: Didelphidae), em Campo Grande, MS In: CONGRESSO DO CENTRO OESTE DOENÇAS INFECCIOSAS, EMERGENTES, REEMERGENTES E NEGLIGENCIADAS, 1., 2007, Campo Grande, MS. Anais... [Campo Grande, MS]: Missão Saúde, [2007]. 1 CD-ROM. Res. 14. CNPGC. Congresso do Centro Oeste DIERN.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 168. | | MIZIARA, S. R.; PAIVA. F.; ANDREOTTI, R.; KOLLER, W. W.; LOPES, V. A.; BITENCOURT, K. Ocorrência de Ixodes loricatus Neumann, 1899 (Acari: Ixodidae) parasitando Didelphis albiventris (Lund, 1841), (Didelphimorphia: Didelphidae), em Campo Grande, MS. Revista Brasileira de Parasitologia Veterinária, v. 17, n. 3, p. 158-160 jul./set. 2008. Nota de pesquisa.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 169. | | MIZIARA, S. R.; PAIVA, F.; KOLLER, W. W.; ANDREOTTI, R.; LOPES, V. A.; PONTES, N. T. Ocorrência de lipoptena (lipoptenella) mazamae rondani, 1878 (diptera, hippoboscidae) parasitando mazama americana (erxleben, 1777) (veado mateiro), em Campo Grande Mato Grosso do Sul, Brasil In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 170. | | ALMEIDA, R. F. C; ANDREOTTI, R.; LABRUNA, M. B; MATOS, M. F. C. Ocorrência de anticorpos anti- rickettsia spp em equinos de Mato Grosso do Sul In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 171. | | ANDREOTTI, R.; PINHEIRO, M. S.; SANTOS, S. A.; AZEVEDO, J. R. M.; BARROS, J. C. Observacoes sobre a preferencia termica de agua pelo jacare do Pantanal (Caiman crocodilus yacare, Daudin, 1802). In: CONGRESSO PANAMERICANO DE CIENCIAS VETERINARIAS, 15., 1996, Campo Grande. Abstracts... Campo Grande: SOMVET-MS / CRMV-MS, 1996. p.82| Biblioteca(s): Embrapa Pantanal. |
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| 174. | | ANDREOTTI, R.; GOMES, A.; PIRES, P. P.; RIVERA, F. E. B. Planejamento sanitario de gado de corte. In: CURSO DE MANEJO SANITARIO, REPRODUTIVO E NUTRICIONAL EM BOVINOS, Programa Repasto - Seprotur, turma 5 - 2. etapa, 2003, Campo Grande. Palestras apresentadas. Campo Grande: Embrapa Gado de Corte, 2003. p. 72-80. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 175. | | OLIVEIRA, J. M. de; MATOS, M. de F. C.; OSHIRO, L. M.; ANDREOTTI, R. Prevalence of anti-Neospora caninum antibodies in dogs in the urban area of Campo Grande, MS, Brazil. Revista Brasileira de Parasitologia Veterinária, São Paulo, v.13, n. 4, p.155-158, out./dez. 2004. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 177. | | GUERRERO, F. D.; LEÓN, A. A. P. de; RODRIGUEZ- VIVAS, R. I.; JONSSON, N.; MILLER, R. J.; ANDREOTTI, R. Acaricide research and development, resistance, and resistance monitoring. In: SONENSHINE, D. E.; ROE, R. M. (Ed.). Biology of ticks. 2nd ed. New York: Oxford, 2014.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 178. | | ANDREOTTI, R.; GUERRERO, F. D.; SOARES, M. A.; BARROS, J. C.; MILLER, R. J.; PÉREZ DE LEÓN, A. Acaricide resistance of Rhipicephalus (Boophilus) microplus in State of Mato Grosso do Sul, Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 20, n. 2, p. 127-133, Apr.-Jun. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 179. | | PINHEIRO, A. F.; MADRI, I. M.; BORSUK, S.; ANDREOTTI, R.; BERME, M. E. A.; LEITE, F. P. L. The adjuvant immune modulation effect in experimental vaccine against Neospora caninum using rNP43 expressed in Pichia pastoris. In: INTERNATIONAL MEETING ON APICOMPLEXAN PARASITES IN FARM ANIMALS, 2012, Lisbon. Apicomplexa in farm animals: proceedings. Lisboa: Escola Superior de Tecnologia da Saúde de Lisboa , 2012. p.131 Poster 66, Apicowplexa2012| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 180. | | GARCIA, M. V.; SANTOS, J. M. dos; AGUIRRE, A. de A. R.; JUAN, M. P.; ANDREOTTI, R. Amblyomma coelebs (Neumann 1899: Acari; Ixodidae) parasitando humanos no Estado de Mato Grosso do Sul, Brasil. In: CONGRESSO DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 50., 2014, Rio Branco., Anais... Rio Branco: SBMT, 2014. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 301 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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