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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/03/2011 |
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Data da última atualização: |
09/09/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ANDREOTTI, R.; SOARES, M.; CUNHA, R. C.; LEITE, F. P. L. |
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Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; UFPEL; UFPEL; UFPEL. |
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Título: |
Heterologous expression in Pichia pastoris of gene BMTI optimized. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010. |
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Páginas: |
p.149-152 |
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Idioma: |
Inglês
Português |
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Notas: |
ICOPA XII |
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Conteúdo: |
The cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was
expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo. MenosThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same... Mostrar Tudo |
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Thesagro: |
Carrapato; Sanidade Animal. |
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Thesaurus Nal: |
Rhipicephalus microplus. |
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Categoria do assunto: |
H Saúde e Patologia
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02512naa a2200217 a 4500
001 1883447
005 2014-09-09
008 2010 bl --- 0-- u #d
100 1 $aANDREOTTI, R.
245 $aHeterologous expression in Pichia pastoris of gene BMTI optimized.
260 $c2010
300 $ap.149-152
500 $aICOPA XII
520 $aThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo.
650 $aRhipicephalus microplus
650 $aCarrapato
650 $aSanidade Animal
700 1 $aSOARES, M.
700 1 $aCUNHA, R. C.
700 1 $aLEITE, F. P. L.
773 $tIn: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
30/01/2023 |
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Data da última atualização: |
02/02/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ZAIDEM, A. L. de M.; FERREIRA, A. L.; RIBEIRO, W. R.; SILVA, A. Y. P. e; SILVA, J. F. A. e; RAGNI, M.; PINHEIRO, P. V. |
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Afiliação: |
ANTONIA LOPES DE MENDONÇA ZAIDEM, estagiária CNPAF; AMANDA LOPES FERREIRA, estagiária CNPAF; WILLIAM RAFAEL RIBEIRO, UNIVERSIDADE FEDERAL DE GOIÁS; ALBERES YANK PEREIRA E SILVA, estagiário CNPAF; JOSE FRANCISCO ARRUDA E SILVA, CNPAF; MIRCO RAGNI, UNIVERSIDADE DE FEIRA DE SANTANA, BA; PATRICIA VALLE PINHEIRO, CNPAF. |
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Título: |
Atração de adultos da mosca branca, Bemisia tabaci, à luz LED em instrumento para o monitoramento automatizado de pragas. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: SEMINÁRIO JOVENS TALENTOS, 16., 2022, Santo Antônio de Goiás. Resumos... Brasília, DF: Embrapa; Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2022. |
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Páginas: |
p. 31. |
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ISBN: |
978-65-89957-37-9 |
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Idioma: |
Português |
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Conteúdo: |
O Manejo Integrado de Pragas (MIP), importante ferramenta para o manejo racional de agrotóxicos, ainda apresenta baixa adoção entre produtores de feijão, devido aos custos de monitoramento. O uso de armadilhas automatizadas pode reduzir significativamente os custos de monitoramento, facilitando a adoção do MIP. O tubo Kalile (tK), desenvolvido para o monitoramento do psilídeo dos citros, é um instrumento de acrílico (50 cm x 4,5 cm x 4,5 cm), com dispositivos de luz LED UV nas duas extremidades. Neste trabalho, estudos foram realizados para validar o uso do tK para atração da mosca branca, Bemisia tabaci, ao LED UV, acoplado ou não a uma placa fosforescente. |
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Palavras-Chave: |
Monitoramento. |
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Thesagro: |
Bemisia Tabaci; Mosca Branca; Praga de Planta. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/246115/1/sjt-p31.pdf
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Marc: |
LEADER 01561nam a2200253 a 4500
001 2151340
005 2023-02-02
008 2022 bl uuuu u00u1 u #d
020 $a978-65-89957-37-9
100 1 $aZAIDEM, A. L. de M.
245 $aAtração de adultos da mosca branca, Bemisia tabaci, à luz LED em instrumento para o monitoramento automatizado de pragas.$h[electronic resource]
260 $aIn: SEMINÁRIO JOVENS TALENTOS, 16., 2022, Santo Antônio de Goiás. Resumos... Brasília, DF: Embrapa; Santo Antônio de Goiás: Embrapa Arroz e Feijão$c2022
300 $ap. 31.
520 $aO Manejo Integrado de Pragas (MIP), importante ferramenta para o manejo racional de agrotóxicos, ainda apresenta baixa adoção entre produtores de feijão, devido aos custos de monitoramento. O uso de armadilhas automatizadas pode reduzir significativamente os custos de monitoramento, facilitando a adoção do MIP. O tubo Kalile (tK), desenvolvido para o monitoramento do psilídeo dos citros, é um instrumento de acrílico (50 cm x 4,5 cm x 4,5 cm), com dispositivos de luz LED UV nas duas extremidades. Neste trabalho, estudos foram realizados para validar o uso do tK para atração da mosca branca, Bemisia tabaci, ao LED UV, acoplado ou não a uma placa fosforescente.
650 $aBemisia Tabaci
650 $aMosca Branca
650 $aPraga de Planta
653 $aMonitoramento
700 1 $aFERREIRA, A. L.
700 1 $aRIBEIRO, W. R.
700 1 $aSILVA, A. Y. P. e
700 1 $aSILVA, J. F. A. e
700 1 $aRAGNI, M.
700 1 $aPINHEIRO, P. V.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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