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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
21/08/2009 |
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Autoria: |
ANDREOLI, C. |
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Título: |
Bioassay method for identifying and detecting genetically modified soybean. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 252. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
There are several commomly used GMO testing protocols, including biological tests, as well as ELISA and PCR tests, for herbicide and insect tolerance. Growers and end-users should consider the advantages and disadvantages of the various testing methods before harvest. Exporters should probably resign themselves to the most rigorous testing protocol, to anticipate the additional scrutiny their products will receive overseas. Large food processors are currently using a combination of tests for identity-preserved (IP) grains. The herbicide bioassays could be used to detect GMO herbicide tolerant traits in Roundup Ready and Liberty Link soybeans and corn. The current test involves placing seeds in a germination media, moistened with a diluted solution containing the herbicide, or spraying the herbicide on seedlings. Seeds that test positive for the presence of the herbicide-tolerant GMO trait will germinate and develop normally, whereas those that die or not develop normally will be GMO-free. Because it is relatively inexpensive, this procedure is widely used by seed producers and grain companies exporting soybeans in the USA. Therefore, the main goal of this research was to test a modified herbicide bioassay for soybean tolerant to glyphosate. Four hundred seeds of conventional soybean, cv. BRS 133 and BRS 214 and RR soybean, cv. BRS Baliza, were imbibed in a solution of 400 ppm glyphosate (i.a = 360gL-1) for 6 hours in the laboratory room. After treatment, seeds were germinated in a paper rolls with four replications at 25º C, for 3 days. The seedlings were classified as a normal, abnormal and susceptible to the herbicide. The trials were replicated six times in order to validate the protocol. The susceptible seedlings presented reduced growth and no secondary roots and the abnormal seedlings are due to low vigor. The RR soybean germinate 96% against 0% (zero) for conventional soybeans. The test showed significant differences between conventional and RR soybean, facilitating the distinction among treatments. The great advantage of this modified protocol was that results could be obtained in three days against six days as it was proposed before. Besides the low cost and a quicker test, the herbicide bioassay seems to be a promising test for detecting GM soybean by seed producers, end-users and exporters. Researchers are being carried out at Embrapa Soja in order to reduce the test time for one day. MenosThere are several commomly used GMO testing protocols, including biological tests, as well as ELISA and PCR tests, for herbicide and insect tolerance. Growers and end-users should consider the advantages and disadvantages of the various testing methods before harvest. Exporters should probably resign themselves to the most rigorous testing protocol, to anticipate the additional scrutiny their products will receive overseas. Large food processors are currently using a combination of tests for identity-preserved (IP) grains. The herbicide bioassays could be used to detect GMO herbicide tolerant traits in Roundup Ready and Liberty Link soybeans and corn. The current test involves placing seeds in a germination media, moistened with a diluted solution containing the herbicide, or spraying the herbicide on seedlings. Seeds that test positive for the presence of the herbicide-tolerant GMO trait will germinate and develop normally, whereas those that die or not develop normally will be GMO-free. Because it is relatively inexpensive, this procedure is widely used by seed producers and grain companies exporting soybeans in the USA. Therefore, the main goal of this research was to test a modified herbicide bioassay for soybean tolerant to glyphosate. Four hundred seeds of conventional soybean, cv. BRS 133 and BRS 214 and RR soybean, cv. BRS Baliza, were imbibed in a solution of 400 ppm glyphosate (i.a = 360gL-1) for 6 hours in the laboratory room. After treatment, seeds were germinate... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 03243naa a2200157 a 4500
001 1466826
005 2009-08-21
008 2004 bl uuuu u00u1 u #d
100 1 $aANDREOLI, C.
245 $aBioassay method for identifying and detecting genetically modified soybean.
260 $c2004
300 $ap. 252.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aThere are several commomly used GMO testing protocols, including biological tests, as well as ELISA and PCR tests, for herbicide and insect tolerance. Growers and end-users should consider the advantages and disadvantages of the various testing methods before harvest. Exporters should probably resign themselves to the most rigorous testing protocol, to anticipate the additional scrutiny their products will receive overseas. Large food processors are currently using a combination of tests for identity-preserved (IP) grains. The herbicide bioassays could be used to detect GMO herbicide tolerant traits in Roundup Ready and Liberty Link soybeans and corn. The current test involves placing seeds in a germination media, moistened with a diluted solution containing the herbicide, or spraying the herbicide on seedlings. Seeds that test positive for the presence of the herbicide-tolerant GMO trait will germinate and develop normally, whereas those that die or not develop normally will be GMO-free. Because it is relatively inexpensive, this procedure is widely used by seed producers and grain companies exporting soybeans in the USA. Therefore, the main goal of this research was to test a modified herbicide bioassay for soybean tolerant to glyphosate. Four hundred seeds of conventional soybean, cv. BRS 133 and BRS 214 and RR soybean, cv. BRS Baliza, were imbibed in a solution of 400 ppm glyphosate (i.a = 360gL-1) for 6 hours in the laboratory room. After treatment, seeds were germinated in a paper rolls with four replications at 25º C, for 3 days. The seedlings were classified as a normal, abnormal and susceptible to the herbicide. The trials were replicated six times in order to validate the protocol. The susceptible seedlings presented reduced growth and no secondary roots and the abnormal seedlings are due to low vigor. The RR soybean germinate 96% against 0% (zero) for conventional soybeans. The test showed significant differences between conventional and RR soybean, facilitating the distinction among treatments. The great advantage of this modified protocol was that results could be obtained in three days against six days as it was proposed before. Besides the low cost and a quicker test, the herbicide bioassay seems to be a promising test for detecting GM soybean by seed producers, end-users and exporters. Researchers are being carried out at Embrapa Soja in order to reduce the test time for one day.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
08/05/1997 |
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Data da última atualização: |
08/05/1997 |
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Autoria: |
KLINK, C. A.; SILVA, J. C. S.; PARRON, L. M. |
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Título: |
Levantamento da bioversidade de gramineas nativas do bioma cerrado. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
In: EMBRAPA. Centro de Pesquisa Agropecuaria dos Cerrados (Planaltina, DF). Relatorio Tecnico Anual do Centro de Pesquisa Agropecuaria dos Cerrados 1991 a 1995. Planaltina, 1997. |
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Páginas: |
p.42-43. |
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Idioma: |
Português |
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Palavras-Chave: |
Indigenous organisms; Planta nativa. |
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Thesagro: |
Biodiversidade; Cerrado; Gramínea. |
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Thesaurus NAL: |
biodiversity; grasses. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00739naa a2200229 a 4500
001 1552825
005 1997-05-08
008 1997 bl uuuu u00u1 u #d
100 1 $aKLINK, C. A.
245 $aLevantamento da bioversidade de gramineas nativas do bioma cerrado.
260 $c1997
300 $ap.42-43.
650 $abiodiversity
650 $agrasses
650 $aBiodiversidade
650 $aCerrado
650 $aGramínea
653 $aIndigenous organisms
653 $aPlanta nativa
700 1 $aSILVA, J. C. S.
700 1 $aPARRON, L. M.
773 $tIn: EMBRAPA. Centro de Pesquisa Agropecuaria dos Cerrados (Planaltina, DF). Relatorio Tecnico Anual do Centro de Pesquisa Agropecuaria dos Cerrados 1991 a 1995. Planaltina, 1997.
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