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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
19/02/2019 |
Data da última atualização: |
19/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
POLETI, M. D.; REGITANO, L. C. de A.; SOUSA, G. H. M. F.; CESAR, A. S. M.; SIMAS, R. C.; SILVA-VIGNATO, B.; OLIVEIRA, G. B.; ANDRADE, S. C. S.; CAMERON, L. C.; COUTINHO, L. L. |
Afiliação: |
Mirele D. Poleti, USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; Gustavo H. M. F. Souza, MS Applications and Development Laboratory Waters Corporation; Aline S. M. Cesar, USP; Rosineide C. Simas, USP; Bárbara Silva-Vignato, USP; Gabriella B. Oliveira, USP; Sónia C. S. Andrade, USP; Luiz C. Cameron, UNIRIO; Luiz Lehmann Coutinho, USP. |
Título: |
Longissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Journal of Proteomics, v. 179, p. 30-41, 2018. |
DOI: |
https://doi.org/10.1016/j.dib.2018.06.004 |
Idioma: |
Inglês |
Conteúdo: |
The pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in human health and meat quality attributes, influencing energy metabolism of skeletal muscle, as well as, tenderness, flavor, and juiciness of beef. We performed for the first time the utilization of integrated transcriptome-assisted label-free quantitative proteomic approach using High Definition Mass Spectrometry for characterization of the changes in the proteomic profile of the Longissimus dorsi muscle associated with intramuscular fat deposition in cattle. Furthermore, we compared the muscle proteome with the muscle transcriptome (mRNA and microRNAs), obtained by RNA-sequencing, to better understand the relationship between expression of mRNAs and proteins and to unravel essential biological mechanisms involved in bovine skeletal muscle IMF deposition. MenosThe pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in... Mostrar Tudo |
Palavras-Chave: |
Adiposidade; Gordura intramuscular; MRNA. |
Thesagro: |
Gordura Animal. |
Thesaurus Nal: |
Mass spectrometry; MicroRNA. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03231naa a2200313 a 4500 001 2106242 005 2019-02-19 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.dib.2018.06.004$2DOI 100 1 $aPOLETI, M. D. 245 $aLongissimus dorsi muscle label-free quantitative proteomic reveals biological mechanisms associated with intramuscular fat deposition.$h[electronic resource] 260 $c2018 520 $aThe pathways involved in intramuscular fat (IMF) deposition in Longissimus dorsi muscle were investigated using an integrated transcriptome-assisted label-free quantitative proteomic approach by High Definition Mass Spectrometry. We quantified 1582 proteins, of which 164 were differentially abundant proteins (DAPs, p < 0.05) between animals with high (H) and low (L) genomic estimated breeding values (GEBV) for IMF content. Ingenuity pathway analysis (IPA) revealed that these DAPs were mainly involved in glycolysis metabolism, actin cytoskeleton signaling, cell-cell adherens junction and pathways for MAPK and insulin. A comparative study between transcriptomic (mRNA) and proteomic data showed 17 differentially expressed genes corresponding to DAPs, of which three genes/proteins did not agree on the direction of the fold change between groups. Moreover, we investigated microRNAs data to explain these differences in fold change direction, being able to unravel two of the three unexpected mRNA/protein relationships. Results demonstrated that changes in protein/mRNA levels of sarcomere organization, intracellular signal transduction and regulation of actin cytoskeleton, are involved in IMF deposition. These findings provide a deeper understanding of the highly complex regulatory mechanisms involved in IMF deposition in cattle and indicate target pathways for future studies. Significance: Intramuscular fat is the amount of fat deposited inside muscle and plays an important role in human health and meat quality attributes, influencing energy metabolism of skeletal muscle, as well as, tenderness, flavor, and juiciness of beef. We performed for the first time the utilization of integrated transcriptome-assisted label-free quantitative proteomic approach using High Definition Mass Spectrometry for characterization of the changes in the proteomic profile of the Longissimus dorsi muscle associated with intramuscular fat deposition in cattle. Furthermore, we compared the muscle proteome with the muscle transcriptome (mRNA and microRNAs), obtained by RNA-sequencing, to better understand the relationship between expression of mRNAs and proteins and to unravel essential biological mechanisms involved in bovine skeletal muscle IMF deposition. 650 $aMass spectrometry 650 $aMicroRNA 650 $aGordura Animal 653 $aAdiposidade 653 $aGordura intramuscular 653 $aMRNA 700 1 $aREGITANO, L. C. de A. 700 1 $aSOUSA, G. H. M. F. 700 1 $aCESAR, A. S. M. 700 1 $aSIMAS, R. C. 700 1 $aSILVA-VIGNATO, B. 700 1 $aOLIVEIRA, G. B. 700 1 $aANDRADE, S. C. S. 700 1 $aCAMERON, L. C. 700 1 $aCOUTINHO, L. L. 773 $tJournal of Proteomics$gv. 179, p. 30-41, 2018.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
19/07/2002 |
Data da última atualização: |
23/05/2005 |
Autoria: |
SILVA, P. S. L. e; BRANDÃO, S. T. |
Título: |
Rendimento no Beneficiamento e Translucidez de Grãos de Cultivares de Arroz em Função de Níveis de Nitrogênio. |
Ano de publicação: |
1987 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, v.22, ns.9/10. p.943-949, set/out. 1987. |
Idioma: |
Português |
Conteúdo: |
Características dos grãos de arroz, como comprimento e largura, translucidez e rendimento no beneficiamento são importantes na determinação do valor do produto para o mercado. Neste estudo, objetivou-se avaliar a influência de níveis de nitrogênio (0, 45 e 90 kg de N/ha), sobre essas características, em cultivares (IAC-435, De Abril, CICA-4, IR-634, IR-661 e IR-665) de arroz irrigado. 0 nitrogênio não influenciou significativamente a largura ou o comprimento dos grãos dos genótipos avaliados nem a proporção de grãos inteiros de IAC-435, CICA 4, IR-634 e IR-661. A adubação teve efeito positivo sobre a proporção de grãos translúcidos de IAC-435, De Abril e IR-665 e sobre o rendimento de inteiros de IR-665, mas alterou negativamente esse rendimento em De Abril. |
Palavras-Chave: |
Barriga branca; Centro branco; comprimento do grão; largura do grão; rendimento de engenho. |
Thesagro: |
Oryza Sativa. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01449naa a2200205 a 4500 001 1107569 005 2005-05-23 008 1987 bl uuuu u00u1 u #d 100 1 $aSILVA, P. S. L. e 245 $aRendimento no Beneficiamento e Translucidez de Grãos de Cultivares de Arroz em Função de Níveis de Nitrogênio. 260 $c1987 520 $aCaracterísticas dos grãos de arroz, como comprimento e largura, translucidez e rendimento no beneficiamento são importantes na determinação do valor do produto para o mercado. Neste estudo, objetivou-se avaliar a influência de níveis de nitrogênio (0, 45 e 90 kg de N/ha), sobre essas características, em cultivares (IAC-435, De Abril, CICA-4, IR-634, IR-661 e IR-665) de arroz irrigado. 0 nitrogênio não influenciou significativamente a largura ou o comprimento dos grãos dos genótipos avaliados nem a proporção de grãos inteiros de IAC-435, CICA 4, IR-634 e IR-661. A adubação teve efeito positivo sobre a proporção de grãos translúcidos de IAC-435, De Abril e IR-665 e sobre o rendimento de inteiros de IR-665, mas alterou negativamente esse rendimento em De Abril. 650 $aOryza Sativa 653 $aBarriga branca 653 $aCentro branco 653 $acomprimento do grão 653 $alargura do grão 653 $arendimento de engenho 700 1 $aBRANDÃO, S. T. 773 $tPesquisa Agropecuária Brasileira, Brasília$gv.22, ns.9/10. p.943-949, set/out. 1987.
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