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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
30/11/2021 |
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Data da última atualização: |
30/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUERRA, P. A.; SOUZA, E. H. de; ANDRADE, E. C. de; MAX, D. de A. S.; OLIVEIRA, R. S.; SOUZA, F. V. D. |
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Afiliação: |
PATRÍCIA ARAÚJO GUERRA, UFRB; EVERTON HILO DE SOUZA, UFRB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; DANIELA DE ANDRADE SILVA MAX, UFRB; RAFAELLE SOUZA DE OLIVEIRA, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Comparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, 2020. |
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ISSN: |
1475-2689 |
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Idioma: |
Inglês |
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Conteúdo: |
The Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed with virus-free plants. MenosThe Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed wi... Mostrar Tudo |
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Thesagro: |
Abacaxi; Planta Ornamental. |
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Categoria do assunto: |
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Marc: |
LEADER 02208naa a2200217 a 4500
001 2136827
005 2021-11-30
008 2020 bl uuuu u00u1 u #d
022 $a1475-2689
100 1 $aGUERRA, P. A.
245 $aComparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties$h[electronic resource]
260 $c2020
520 $aThe Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed with virus-free plants.
650 $aAbacaxi
650 $aPlanta Ornamental
700 1 $aSOUZA, E. H. de
700 1 $aANDRADE, E. C. de
700 1 $aMAX, D. de A. S.
700 1 $aOLIVEIRA, R. S.
700 1 $aSOUZA, F. V. D.
773 $tIn Vitro Cellular & Developmental Biology - Plant, 2020.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 4. |  | MENDONÇA-SANTOS, M. de L.; SANTOS, H. G. dos; DART, R. de O.; PARES, J. G. Digital mapping of soil classes in Rio de Janeiro State, Brazil: data, modelling and prediction. In: HARTEMINK, A. E.; McBRATNEY, A.; MENDONÇA-SANTOS, M. de L. (ed.). Digital soil mapping with limited data. Dordrecht: Springer, 2008. cap. 34, p. 381-396.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Solos. |
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| 8. | | DART, R. de O.; COELHO, M. R.; MENDONÇA-SANTOS, M. de L.; PARES, J. G.; BERBARA, R. L. L. Digital soil mapping at Parque Estadual da Mata Seca, Minas Gerais state, Brazil: applying regression tree to predict soil classes. In: INTERNATIONAL WORKSHOP ON DIGITAL SOIL MAPPING, 4., 2010, Rome. From digital soil mapping to digital soil assessment: identifying key gaps from fields to continents: proceedings... Rome: IUSS, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 9. | | ANDRADE, S. F. de; MENDONÇA-SANTOS, M. de L.; CARVALHO, C. N. de; DART, R. de O.; PARES, J. G. Digital soil fertility mapping of the North, Northwest and Serrana state of Rio de Janeiro. In: INTERNATIONAL WORKSHOP ON DIGITAL SOIL MAPPING, 4., 2010, Rome. From digital soil mapping to digital soil assessment: identifying key gaps from fields to continents: proceedings... Rome: IUSS, 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 10. | | FILGUEIRAS, P. R.; SOUZA, A. M. de; POPPI, R. J.; COELHO, M. R.; PARÉS, J. G.; CUNHA, T. A. F.; DART, R. de O. Avaliação de modelos de calibração PLS e SVM na determinação do carbono orgânico do solo por espectroscopia NIR. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 35., 2012, Águas de Lindóia. Responsabilidade, ética e progresso social: trabalhos. [São Paulo]: Sociedade Brasileira de Química, 2012. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 11. | | SOUZA, A. M. de; COELHO, M. R.; FIGUEIRAS, P.; CUNHA, T. A. F.; DART, R. de O.; PARÉS, J. G.; SIMON, P. L.; CRUZ, B. G. da; POPPI, R. J.; MENDONÇA-SANTOS, M. de L.; BERBARA, R. L. L. Proposta de tutorial de quimiometria utilizando técnicas modernas para a análise de solos. Revista Homem, Espaço e Tempo, Sobral, ano 5, n. 2, set. 2012. Edição dos Anais do VI Simpósio Brasileiro de Educação em Solos, Sobral, maio 2012. 15 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 12. | | SANTOS, H. G. dos; CARVALHO JUNIOR, W. de; DART, R. de O.; AGLIO, M. L. D.; SOUZA, J. S. de; PARES, J. G.; FONTANA, A.; MARTINS, A. L. da S.; OLIVEIRA, A. P. de. O novo mapa de solos do Brasil: legenda atualizada. Rio de Janeiro: Embrapa Solos, 2011. 67 p. (Embrapa Solos. Documentos, 130). Acompanha 1 mapa, color. Escala 1:5.000.000.| Biblioteca(s): Embrapa Solos. |
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| Registros recuperados : 12 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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