Data/hora: 17/06/2024 02:10:42

Biblioteca(s): 	Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja.	
Data corrente: 	07/07/2022	
Data da última atualização: 	08/07/2022	
Autoria: 	TÁVORA, F. T. P. K.; DINIZ, F. de A. dos S.; RÊGO-MACHADO, C. de M.; FREITAS, N. C.; ARRAES, F. B. M.; ANDRADE, E. C. de; FURTADO, L. L.; OSIRO, K. O.; SOUSA, N. L. de; CARDOSO, T. B.; MERTZ-HENNING, L. M.; OLIVEIRA, P. A. de; FEINGOLD, S. E.; HUNTER, W. B.; SA, M. F. G. de; KOBAYASHI, A. K.; NEPOMUCENO, A. L.; SANTIAGO, T. R.; MOLINARI, H. B. C.	
Afiliação: 	FABIANO TOUZDJIAN PINHEIRO KOHLRAUSCH TÁVORA, UNB; FRANCISCO DE ASSIS DOS SANTOS DINIZ, UNB; CAMILA DE MORAES RÊGO-MACHADO, UNB; NATÁLIA CHAGAS FREITAS; FABRÍCIO BARBOSA MONTEIRO ARRAES; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; LEILA LOURENÇO FURTADO, UNB; KAREN OFUJI OSIRO, UNB; NATÁLIA LIMA DE SOUSA, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; THIAGO BÉRGAMO CARDOSO, SEMPRE AgTech; LILIANE MARCIA MERTZ HENNING, CNPSO; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; SÉRGIO ENRIQUE FEINGOLD, IPADS-Balcarce (UEDD INTA-CONICET), Argentina; WAYNE B. HUNTER, USDA-ARS, United States; MARIA FATIMA GROSSI DE SA, Cenargen; ADILSON KENJI KOBAYASHI, CNPAE; ALEXANDRE LIMA NEPOMUCENO, CNPSO; THAÍS RIBEIRO SANTIAGO, UNB; HUGO BRUNO CORREA MOLINARI, SEMPRE AgTech.	
Título: 	CRISPR/Cas- and Topical RNAi-Based Technologies for crop management and improvement: reviewing the risk assessment and challenges towards a more sustainable agriculture.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Frontiers in Bioengineering and Biotechnology, v. 10, 2022. Article 913728.	
DOI: 	https://doi.org/10.3389/fbioe.2022.913728	
Idioma: 	Inglês	
Conteúdo: 	Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research and breeding. In recent years, the use of these modern technologies has been explored in various sectors of agriculture, introducing or improving important agronomic traits in plant crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, the limitations of each technique, public perception, and regulatory aspects are hindering its wide adoption for the development of new crop varieties or products. In an attempt to reverse these mishaps, scientists have been researching alternatives to increase the specificity, uptake, and stability of the CRISPR and RNAi system components in the target organism, as well as to reduce the chance of toxicity in nontarget organisms to minimize environmental risk, health problems, and regulatory issues. In this review, we discuss several aspects related to risk assessment, toxicity, and advances in the use of CRISPR/Cas and topical RNAi-based technologies in crop management and breeding. The present study also highlights the advantages and possible drawbacks of each technology, provides a brief overview of how to circumvent the off-target occurrence, the strategies to increase on-target specificity, the harm/benefits of association with nanotechnology, the public perception of the available techniques, worldwide regulatory frameworks regarding topical RNAi and CRISPR technologies, and, lastly, presents successful case studies of biotechnological solutions derived from both technologies, raising potential challenges to reach the market and being social and environmentally safe.	
Palavras-Chave: 	Exogenous dsRNA; Genome editin; Genome editing; Offtargets; Public acceptance; Regulatory aspects.	
Thesagro: 	Genoma.	
Thesaurus Nal: 	Gene silencing; Nanotechnology; Toxicity.	
Categoria do assunto: 	--
X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)	
Exemplares: 	CENARGEN - (38875-1) - UPC - AP - DD -  -  -  - DIS
CNPAE - (4052-1) - UPC - AP - DD -  -  -  - DIS
CNPSO - (40501-1) - UPC - AP - DD -  -  -  - DIS