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Registro Completo |
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Biblioteca(s): |
Embrapa Agrossilvipastoril; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
28/03/2022 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAULA, D. P.; BARROS, S. K. A.; PITTA, R. M.; BARRETO, M. R.; TOGAWA, R. C.; ANDOW, D. A. |
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Afiliação: |
DEBORA PIRES PAULA, Cenargen; SUELLEN KARINA ALBERTONI BARROS, UFMT; RAFAEL MAJOR PITTA, CPAMT; MARLITON ROCHA BARRETO, UFMT; ROBERTO COITI TOGAWA, Cenargen; DAVID A. ANDOW, UNIVERSITY OF MINNESOTA. |
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Título: |
Metabarcoding versus mapping unassembled shotgun reads for identification of prey consumed by arthropod epigeal predators. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
GigaScience, v. 11, n. 1, p. 1-13, 2022. |
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DOI: |
https://doi.org/10.1093/gigascience/giac020 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: A central challenge of DNA gut content analysis is to identify prey in a highly degraded DNA community. In this study, weevaluated prey detection using metabarcoding and a method of mapping unassembled shotgun reads (Lazaro). Inamockpreycommunity,metabarcodingdidnotdetectanyprey,probablyowingtoprimerchoiceand/orpreferentialpredator DNA amplification, while Lazaro detected prey with accuracy 43?71%. Gut content analysis of field-collected arthropod epigeal predators (3 ants, 1 dermapteran, and 1 carabid) from agricultural habitats in Brazil (27 samples, 46?273 individuals per sample) revealed that 64% of the prey species detections by either method were not confirmed by melting curve analysis and 87% of the true prey were detected in common. We hypothesized that Lazaro would detect fewer true- and false-positive and more false-negative prey with greater taxonomic resolution than metabarcoding but found that the methods were similar in sensitivity, specificity, false discovery rate, false omission rate, and accuracy.There was a positive correlation between the relative prey DNA concentration in the samples and the number of prey reads detected by Lazaro,while this was inconsistent for metabarcoding. Metabarcoding and Lazaro had similar,but partially complementary,detection of prey in arthropod predator guts.However, while Lazaro wasalmost2×moreexpensive,thenumberofreadswasrelatedtotheamountofpreyDNA,suggestingthatLazaro mayprovide quantitative prey information while metabarcoding did not. MenosAbstract: A central challenge of DNA gut content analysis is to identify prey in a highly degraded DNA community. In this study, weevaluated prey detection using metabarcoding and a method of mapping unassembled shotgun reads (Lazaro). Inamockpreycommunity,metabarcodingdidnotdetectanyprey,probablyowingtoprimerchoiceand/orpreferentialpredator DNA amplification, while Lazaro detected prey with accuracy 43?71%. Gut content analysis of field-collected arthropod epigeal predators (3 ants, 1 dermapteran, and 1 carabid) from agricultural habitats in Brazil (27 samples, 46?273 individuals per sample) revealed that 64% of the prey species detections by either method were not confirmed by melting curve analysis and 87% of the true prey were detected in common. We hypothesized that Lazaro would detect fewer true- and false-positive and more false-negative prey with greater taxonomic resolution than metabarcoding but found that the methods were similar in sensitivity, specificity, false discovery rate, false omission rate, and accuracy.There was a positive correlation between the relative prey DNA concentration in the samples and the number of prey reads detected by Lazaro,while this was inconsistent for metabarcoding. Metabarcoding and Lazaro had similar,but partially complementary,detection of prey in arthropod predator guts.However, while Lazaro wasalmost2×moreexpensive,thenumberofreadswasrelatedtotheamountofpreyDNA,suggestingthatLazaro mayprovide quantitative prey information while ... Mostrar Tudo |
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Palavras-Chave: |
Diet analysis; Environmental DNA; Generalist predators; Gut content analysis. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232999/1/giac020.pdf
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Marc: |
LEADER 02285naa a2200241 a 4500
001 2151304
005 2023-01-27
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1093/gigascience/giac020$2DOI
100 1 $aPAULA, D. P.
245 $aMetabarcoding versus mapping unassembled shotgun reads for identification of prey consumed by arthropod epigeal predators.$h[electronic resource]
260 $c2022
520 $aAbstract: A central challenge of DNA gut content analysis is to identify prey in a highly degraded DNA community. In this study, weevaluated prey detection using metabarcoding and a method of mapping unassembled shotgun reads (Lazaro). Inamockpreycommunity,metabarcodingdidnotdetectanyprey,probablyowingtoprimerchoiceand/orpreferentialpredator DNA amplification, while Lazaro detected prey with accuracy 43?71%. Gut content analysis of field-collected arthropod epigeal predators (3 ants, 1 dermapteran, and 1 carabid) from agricultural habitats in Brazil (27 samples, 46?273 individuals per sample) revealed that 64% of the prey species detections by either method were not confirmed by melting curve analysis and 87% of the true prey were detected in common. We hypothesized that Lazaro would detect fewer true- and false-positive and more false-negative prey with greater taxonomic resolution than metabarcoding but found that the methods were similar in sensitivity, specificity, false discovery rate, false omission rate, and accuracy.There was a positive correlation between the relative prey DNA concentration in the samples and the number of prey reads detected by Lazaro,while this was inconsistent for metabarcoding. Metabarcoding and Lazaro had similar,but partially complementary,detection of prey in arthropod predator guts.However, while Lazaro wasalmost2×moreexpensive,thenumberofreadswasrelatedtotheamountofpreyDNA,suggestingthatLazaro mayprovide quantitative prey information while metabarcoding did not.
653 $aDiet analysis
653 $aEnvironmental DNA
653 $aGeneralist predators
653 $aGut content analysis
700 1 $aBARROS, S. K. A.
700 1 $aPITTA, R. M.
700 1 $aBARRETO, M. R.
700 1 $aTOGAWA, R. C.
700 1 $aANDOW, D. A.
773 $tGigaScience$gv. 11, n. 1, p. 1-13, 2022.
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Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
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Biblioteca |
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| Registros recuperados : 52 | |
| 22. |  | ANDOW, D. A.; RESENDE FILHO, M. A.; CARNEIRO, R. G.; LORENA, D. R.; SUJII, E. R.; ALVES, R. T. Heterogeneity in intention to adopt organic strawberry production practices among producers in the Federal District, Brazil. Ecological Economics, October, v. 140, p. 177-189 , 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 23. | | PAULA, D. P.; LINARD, B.; ANDOW, D. A.; SUJII, E. R.; PIRES, C. S. S.; VOGLER, A. P. Detection and decay rates of prey and prey symbionts in the gut of a predator through metagenomics. Molecular Ecology Resources, v. 15, n. 4, p. 880-892, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 24. | | RESENDE FILHO, M. A.; ANDOW, D. A.; CARNEIRO, R. G.; LORENA, D. R.; SUJII, E. R.; ALVES, R. T. Economic and productivity incentives to produce organically in Brazil: Evidence from strawberry production in the Federal District. Renewable Agriculture and Food Systems, v. 34, n. 2, p. 155-168, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 25. | | PAULA, D. P.; BARROS, S. K. A.; PITTA, R. M.; BARRETO, M. R.; TOGAWA, R. C.; ANDOW, D. A. Metabarcoding versus mapping unassembled shotgun reads for identification of prey consumed by arthropod epigeal predators. GigaScience, v. 11, n. 1, p. 1-13, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Recursos Genéticos e Biotecnologia. |
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| 28. | | LIMA, D. D.; SOUSA, A. A. T. C. de; FONTES, E. M. G.; SUJII, E. R.; ANDOW, D. A.; PIRES, C. S. S. Abundância de Harmonia axyridis e das joaninhas locais em produção orgânica de brássicas no Distrito Federal. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., Gramado. Saúde, ambiente e agricultura: anais. Gramado: SEB, 2018. 446 p. Na publicação: Alex Antônio T. C. Sousa.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 29. | | LIMA, D. D.; SILVA, A. C. da; FONTES, E. M. G.; PIRES, C. S. S.; SUJII, E. R.; PAULA, D. P.; SOUZA, L. M. de; ANDOW, D. A. Aceitabilidade de joaninhas predadoras a diferentes espécies de pulgões (Coleoptera: Coccinellidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., Gramado. Saúde, ambiente e agricultura: anais. Gramado: SEB, 2018. Na publicação: Lucas M. Souza.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 30. | | PAULA, D. P.; SOUZA, L. M. de; ANDOW, D. A.; SOUSA, A. A. T. C. de; PIRES, C. S. S.; SUJII, E. R. Artificial tritrophic exposure system for environmental risk analysis on aphidophagous predators. Anais da Academia Brasileira de Ciências, v. 88, n. 3, p. 1569-1575, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 31. | | FARIA, M. R. de; LUNDGREN, J. G.; FONTES, E. M. G.; FERNANDES, O. A.; SCHMIDT, F.; VAN TUAT, N.; ANDOW, D. A. Assessing the effects of Bt cotton on generalist arthropod predators. In: HILBECK, A.; ANDOW, D. A.; FONTES, E. M. G.; KAPUSCINSKI, A. R.; SCHEI, P. J. (Ed.). Environmental risk assessment of genetically modified organisms: methodologies for assessing Bt cotton in Brazil. Wallingford, UK: CABI Publishing, 2006. v. 2. p. 175-199. (Environmental risk assessment of genetically modified organisms series, v. 2).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 32. | | ANDOW, D. A.; BARROSO, P. A. V.; FONTES, E. M. G.; GROSSI-DE-SÁ, M. F.; HILBECK, A.; FITT, G. P. Improving the scientific basis for environmental risk assessment through the case study of Bt cotton in Brazil. In: HILBECK, A.; ANDOW, D. A.; FONTES, E. M. G.; KAPUSCINSKI, A. R.; SCHEI, P. J. (Ed.). Environmental risk assessment of genetically modified organisms: methodologies for assessing Bt cotton in Brazil. Wallingford, UK: CABI Publishing, 2006. v. 2. p.1-20. (Environmental risk assessment of genetically modified organisms series, v. 2).| Biblioteca(s): Embrapa Algodão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 33. | | HILBECK, A.; ANDOW, D. A.; ARPAIA, S.; BIRCH, A. N. E.; FONTES, E. M. G.; LÖVEI, G. L.; SUJII, E. R.; WHEATLEY, R. E.; UNDERWOOD, E. Methodology to support non-target and biodiversity risk assessment. In: HILBECK, A.; ANDOW, D. A.; FONTES, E. M. G.; KAPUSCINSKI, A. R.; SCHEI, P. J. (Ed.). Environmental risk assessment of genetically modified organisms: methodologies for assessing Bt cotton in Brazil. Wallingford, UK: CABI Publishing, 2006. v. 2. p. 108-132. (Environmental risk assessment of genetically modified organisms series, v. 2).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 34. | | PAULA, D. P.; ANDOW, D. A.; BELLINATI, A.; TIMBÓ, R. V.; SOUZA, L. M.; PIRES, C. S. S.; SUJII, E. R. Limitations in dose?response and surrogate species methodologies for risk assessment of Cry toxins on arthropod natural enemies. Ecotoxicology, v. 25, p. 601-607, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 35. | | FITT, G. P.; OMOTO, C.; MAIA, A. de H. N.; WAQUIL, J. M.; CAPRIO, M.; OKECH, M. A.; CIA, E.; NGUYEN, H. H.; ANDOW, D. A. Resistance risks of Bt cotton and thei management in Brazil. In: HILBECK, A.; ANDOW, D. A. FONTES, E. M. G. Methodologies for assessing Bt cotton in Brazil. In: KAPUSCINSKI, A. R.; SCHEI, P. J. Environmmental risk assessment of genetically modified organisms. WALLINGFORD: CABI PUBLISHING, 2006. v. 2, p. 300-345.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 36. | | FITT, G. P.; OMOTO, C.; MAIA, A. H.; WAQUIL, J. M.; CAPRIO, M.; OKECH, M. A.; CIA, E.; HUAN, N. H.; ANDOW, D. A. Resistance risks of Bt cotton and their management in Brazil. In: HILBECK, A.; ANDOW, D.A.; FONTES, E. M. G. (Ed.). Environmental risk assessment of genetically modified organisms. Wallingford: CAB International, 2006. v. 2, p. 300-345.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 37. | | PAULA, D. P.; TOGNI, P. H. B.; COSTA, V. A.; SOUZA, L. M.; SOUSA, A. A. T. C.; TOSTES, G. M.; PIRES, C. S. S.; ANDOW, D. A. Scrutinizing the enemy release hypothesis: population effects of parasitoids on Harmonia axyridis and local host coccinellids in Brazil. BioControl, v. 66, n. 1, p. 71-82, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 38. | | SOUZA, C. S. F.; MENDES, S. M.; PAULA, D. P.; ANDOW, D. A.; SILVEIRA, L. C. P.; DAMASCENO, N. C. R.; TEATINI, B. C.; SOUZA, B. H. S. Sequestro e transferência da proteína Cry1F da planta para os ovos por um lepidóptero não aposemático. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., 2018, Gramado. Saúde, ambiente e agricultura: anais. Gramado: SEB, 2018. p. 1163.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 39. | | SOUZA, C. S.; MENDES, S. M.; PAULA, D. P.; ANDOW, D. A.; SILVEIRA, L. C. P.; NATHALIA C. R. DAMASCENO; TEATINI, B. C.; SOUZA, B. H. S. Sequestro e transferência da proteína Cry1F da planta para os ovos por um lepidóptero não aposemático. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., Gramado. Saúde, ambiente e agricultura: anais. Gramado: SEB, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 40. | | NGUYEN, T. T. C.; SUJII, E. R.; WILSON, L. J.; UNDERWOOD, E.; ANDOW, D. A.; MAI, V. H.; BAOPING, Z.; HO, V. C. Potential effect of transgenic cotton on non-target herbivores In Vietnam. In: ANDOW, D. A.; HILBECK, A.; TUAT, N. V. (Ed.) Environmental risk assessment of genetically modified organisms: challenges and opportunities with BT cotton in Vietnam. Wallingford, UK: CABI Publishing, 2008. v. 4 360 p. (Environmental risk assessment of genetically modified organisms series, v. 4). p. 138-175.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 52 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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