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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
13/01/2009 |
Data da última atualização: |
14/01/2009 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MACHADO, M. A.; ÁSTUA, J. F.; AMARAL, A. M. do; MALOSSO, A.; MAZZAFERA, P. |
Afiliação: |
M. A. Machado, APTA; Juliana Freitas-Ástua, CNPMF/APTA; A. M. do Amaral, APTA; A. Malosso, APTA; P. Mazzafera, APTA. |
Título: |
Gene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.). |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105. |
Idioma: |
Inglês |
Conteúdo: |
Varieties of sweet orange are usually distinguishable by the content and quality of the juice. Such traits are important to characterize early, middle and late varieties. Juice quality is a complex trait that changes according with the content and quality of carotenoids, flavanoids, sugars and organic acids. The balance among such compounds is the main characteristic typifies the fruits of almost all varieties of sweet orange. The studies of biochemical pathway of carotenoid synthesis are hindered by the difficulties to evaluate enzyme activities which substracts and products are hydrophobic compounds. Thus the goal of this work was to evaluate the expression of genes of carotenoid synthesis in fruits of sweet orange Hamlin (early), Pera (middle), and Valencia (late), and to correlate with the content of carotenoids during fruit development. A pool of three fruits of 12 years old trees of sweet orange grafted on Rangpur lime was evaluate for fruit quality (juice content, Brix, ratio, content of total carotenoids), gene expression by Rt-qPCR in flavedo (epicarp) (Phytoene synthase, psy; Phytoene desaturase, pds; Z-carotene desaturase, zds; Carotenoid isomerase, crtiso; Lycopene cyclase, lcyb; Beta-hydroxylase, hyb; Zexanthin epoxidase, zep; Lycopene cyclase, lcye). Primers design was based on CitEST and public database. Rt-qPCR was performed using Sybergreen. The higher gene expression (7 to 19 times) was in sweet orange Hamlin at the end of the season (June, in Brazil), pointed out an active synthesis varieties the higher expression was in the middle of the cycle (July) before the picking time. Not always a direct relationship between gene expression and content of carotenoids could be inferred. MenosVarieties of sweet orange are usually distinguishable by the content and quality of the juice. Such traits are important to characterize early, middle and late varieties. Juice quality is a complex trait that changes according with the content and quality of carotenoids, flavanoids, sugars and organic acids. The balance among such compounds is the main characteristic typifies the fruits of almost all varieties of sweet orange. The studies of biochemical pathway of carotenoid synthesis are hindered by the difficulties to evaluate enzyme activities which substracts and products are hydrophobic compounds. Thus the goal of this work was to evaluate the expression of genes of carotenoid synthesis in fruits of sweet orange Hamlin (early), Pera (middle), and Valencia (late), and to correlate with the content of carotenoids during fruit development. A pool of three fruits of 12 years old trees of sweet orange grafted on Rangpur lime was evaluate for fruit quality (juice content, Brix, ratio, content of total carotenoids), gene expression by Rt-qPCR in flavedo (epicarp) (Phytoene synthase, psy; Phytoene desaturase, pds; Z-carotene desaturase, zds; Carotenoid isomerase, crtiso; Lycopene cyclase, lcyb; Beta-hydroxylase, hyb; Zexanthin epoxidase, zep; Lycopene cyclase, lcye). Primers design was based on CitEST and public database. Rt-qPCR was performed using Sybergreen. The higher gene expression (7 to 19 times) was in sweet orange Hamlin at the end of the season (June, in Brazil), poi... Mostrar Tudo |
Palavras-Chave: |
Citros. |
Thesagro: |
Variedade; Vitamina. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02435naa a2200205 a 4500 001 1655661 005 2009-01-14 008 2008 bl uuuu u00u1 u #d 100 1 $aMACHADO, M. A. 245 $aGene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.). 260 $c2008 520 $aVarieties of sweet orange are usually distinguishable by the content and quality of the juice. Such traits are important to characterize early, middle and late varieties. Juice quality is a complex trait that changes according with the content and quality of carotenoids, flavanoids, sugars and organic acids. The balance among such compounds is the main characteristic typifies the fruits of almost all varieties of sweet orange. The studies of biochemical pathway of carotenoid synthesis are hindered by the difficulties to evaluate enzyme activities which substracts and products are hydrophobic compounds. Thus the goal of this work was to evaluate the expression of genes of carotenoid synthesis in fruits of sweet orange Hamlin (early), Pera (middle), and Valencia (late), and to correlate with the content of carotenoids during fruit development. A pool of three fruits of 12 years old trees of sweet orange grafted on Rangpur lime was evaluate for fruit quality (juice content, Brix, ratio, content of total carotenoids), gene expression by Rt-qPCR in flavedo (epicarp) (Phytoene synthase, psy; Phytoene desaturase, pds; Z-carotene desaturase, zds; Carotenoid isomerase, crtiso; Lycopene cyclase, lcyb; Beta-hydroxylase, hyb; Zexanthin epoxidase, zep; Lycopene cyclase, lcye). Primers design was based on CitEST and public database. Rt-qPCR was performed using Sybergreen. The higher gene expression (7 to 19 times) was in sweet orange Hamlin at the end of the season (June, in Brazil), pointed out an active synthesis varieties the higher expression was in the middle of the cycle (July) before the picking time. Not always a direct relationship between gene expression and content of carotenoids could be inferred. 650 $aVariedade 650 $aVitamina 653 $aCitros 700 1 $aÁSTUA, J. F. 700 1 $aAMARAL, A. M. do 700 1 $aMALOSSO, A. 700 1 $aMAZZAFERA, P. 773 $tIn: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registros recuperados : 53 | |
4. | | AMARAL, A. M. do; HOMEM, R. A.; MACHADO, M. A. Expressão de genes de Xanthomonas axonopodis pv. citri através de interação com a planta de citros. Fitopatologia Brasileira, Brasília, DF, v. 32, supl., p. S247, ago. 2007. Resumo 0692. Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, PR, ago. 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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5. | | HOMEM, R. A.; BAPTISTA, J. C.; AMARAL, A. M. do; MACHADO, M. A. Análise mutacional de Xanthomonas axonopodis pv. citri para componentes do sistema de secreção tipo III. Summa Phytopathologica, Botucatu, v. 33, supl., p. S14, 2007. Resumo 020. Edição dos Anais do XXX Congresso Paulista de Fitopatologia, 2007, Jaboticabal.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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10. | | MACHADO, M. A.; ÁSTUA, J. F.; AMARAL, A. M. do; MALOSSO, A.; MAZZAFERA, P. Gene expression of carotenoid biosynthesis pathway and carotenoid content in different varieties of sweet orange (Citrus sinensis L. Osb.). In: INTERNATIONAL CITRUS CONGRESS, 11., 2008, Wuhan,China. Program and abstracts... Wuhan: The International Society of Citriculture, 2008. p. 60. 105.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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15. | | AMARAL, A. M. do; DOMINGUES, A. D.; MACHADO, M. A.; CARVALHO, S. A. de. Resposta ao cancro cítrico, em casa-de-vegetação, de plantas de citros de variedades com potencial uso comercial. Fitopatologia Brasileira, Brasília, DF, v. 29, p. S242, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | AMARAL, A. M. do; BAPTISTA, J. C.; SOUZA, C. B. de; RONCOLETTA, J. G. T.; MACHADO, M. A. Adaptação patógeno-hospedeiro e virulência de mutante de Xanthomonas axonopodis pv. citri defectivo para produção de pigmento. Fitopatologia Brasileira, Brasília, DF, v. 29, p. S253, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | CARVALHO, S. A.; CROCE-FLIHO, J.; NUNES, W. M.; AMARAL, A. M. do; SILVA, L. F. C.; MACHADO, M. A. Avaliação da resistência ao cancro cítrico em germoplasma de citros em condições de campo. Fitopatologia Brasileira, v. 30, supl., ago., 2005.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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20. | | AMARAL, A. M. do; BAPTISTA, J. C.; BUENO, C. de S.; RONCOLETTA, J. G. T.; MACHADO, M. A. Genome-wide screening for genes functionally related to pathogenicity in Xanthomonas axonopodis pv. citri, the casual agent of Citrus Canker. In: PLANT & ANIMAL GENOME, 13., 2005, San Diego, USA. Abstracts... San Diego: Scherago International, 2005. p. 252Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 53 | |
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