Data/hora: 23/06/2024 17:24:20

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 17/07/2009
Data da última atualização: | 14/06/2023
Autoria: | ALVES-JÚNIOR, M.; ALFENAS-ZERBINI, P.; ANDRADE, E. C. de; ESPOSITO, D. A.; SILVA, F. N.; CRUZ, A. C. F. da; VENTRELLA, M. C.; OTONI, W. C.; ZERBINI, F. M.
Afiliação: | Miguel Alves-Júnior, UFV; Poliane Alfenas-Zerbini, UFV; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; Débora A. Esposito, UFV; Fábio N. Silva, UFV; Ana Cláudia F. da Cruz, UFV; Marília C. Ventrella, UFV; Wagner C. Otoni, UFV; F. Murilo Zerbini, UFV.
Título: | Synergism and negative interference during co-infection of tomato and Nicotiana benthamiana with two bipartite begomoviruses.
Ano de publicação: | 2009
Fonte/Imprenta: | Virology, v. 387, n. 2, p. 257-266, mai. 2009.
ISSN: | 0042-6822
DOI: | DOI: doi:10.1016/j.virol.2009.01.046
Idioma: | Inglês
Conteúdo: | In Brazil, at least eight begomoviruses including Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV) infect tomatoes. ToYSV symptoms in tomato and Nicotiana benthamiana appear earlier and are more severe compared to those of ToRMV. We investigated the role of several factors in this differential adaptation. To analyze infection kinetics, a single leaf was inoculated and subsequently detached after different periods of time. Viral DNA accumulation was quantified in plants, viral replication was analyzed in protoplasts, and tissue tropism was determined by in situ hybridization. Results indicate that ToYSV establishes a systemic infection and reaches a higher concentration earlier than ToRMV in both hosts. ToRMV negatively interferes with ToYSV during the initial stages of infection, but once systemic infection is established this interference ceases. In N. benthamiana, ToYSV invades the mesophyll, while ToRMV is phloem-restricted. During dual infection in this host, ToYSV releases ToRMV from the phloem.
Palavras-Chave: | Geminivirus; Plant-virus interaction; Tomato; ToRMV; ToYSV.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (25709-1) - UPC - AP - DD - Publicação digital -  -  - DIS